Process for separating tea sapogenin and tea polysaccharide from tea-oil cake

A technology of tea saponin and tea polysaccharide, which is applied in the fields of food and medicine, can solve the problems of low market price of tea saponin, high production cost, complex process, etc., and achieve easy large-scale industrial production, low production cost, and high white purity Effect

Inactive Publication Date: 2007-11-14
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shortcoming of the present invention: because have used water-containing lower alcohol to make solvent, contain pectin and tea sugar in tea saponin, therefore, purity is not high
Shortcoming of the present invention: because have used water to make solvent, contain pectin and tea sugar in the tea saponin, therefore, purity is not high; When concentrating in addition, the equipment capital that needs is high; The energy consumption is high
The disadvantages of the present invention: because the styrene-type copolymer resin is used to absorb the tea saponin in the tea cake leaching liquid, the process is complicated and the production cost is high; the market price of the tea saponin is low, and lacks commercial competitive advantage
Shortcoming of the present invention: the present invention uses gasoline to wash tea saponin, and its security is not high
Shortcoming of the present invention: because have used water to make solvent, contain pectin and tea sugar in the tea saponin, therefore, purity is not high; When concentrating in addition, the equipment capital that needs is high; The energy consumption is high
Shortcoming of the present invention: because have used water to make solvent, contain pectin and tea sugar in the tea saponin, therefore, purity is not high; When concentrating in addition, the equipment capital that needs is high; The energy consumption is high
Disadvantages of the present invention: the equipment required is relatively high; and whether the powerful ultrasonic waves are harmful to the human body during industrial production remains to be studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Tea oil cake 200---300 mesh, 615 grams, placed in a 2-liter round bottom flask, added as 1500 ml of absolute ethanol, refluxed in a water bath for 4.5 hours; filtered, and the filter residue was refluxed for a total of 8 times. Combine the ethanol solutions and concentrate at 600C under reduced pressure to form an extract; extract the extract with n-butanol at 1200C and cool at 00C to obtain tea saponin solid and n-butanol solution; under reduced pressure, recover n-butanol Alcoholic solution to obtain a mixture containing tea polysaccharides. The above-mentioned tea saponin solid was extracted again at 120°C with n-butanol, cooled at 0°C, and filtered to obtain 78 grams of tea saponin white solid, with a content of 91%; the n-butanol solution was recovered to obtain a mixture containing tea polysaccharides. Combine the mixture containing tea polysaccharides, add (V / V) 95% chemically pure ethanol 1200 ml, let stand for 1 day, filter to obtain 2.5 grams of tea polysaccha...

Embodiment 2

[0041] 605 grams of tea oil cake 200---300 mesh, placed in a 2-liter round bottom flask, added with a concentration of 1500 ml of anhydrous methanol, refluxed in a water bath for 4.5 hours; filtered, and the filter residue was repeated for a total of 8 times of methanol reflux. Combine the methanol solutions, concentrate at 600C under reduced pressure to form an extract; extract the extract with n-butanol at 1300C, and cool at 100C to obtain tea saponin solid and n-butanol solution; under reduced pressure, recover n-butanol Alcoholic solution to obtain a mixture containing tea polysaccharides. The above-mentioned tea saponin solid was extracted again at 130°C with n-butanol, cooled at 100°C, and filtered to obtain 70.5 grams of tea saponin white solid, with a content of 92.5%; reclaim the n-butanol solution to obtain a mixture containing tea polysaccharides. Combine the mixture containing tea polysaccharides, add (V / V) 90% chemically pure ethanol 1200 ml, let stand for 1 day, ...

Embodiment 3

[0043] Tea oil cake 200---300 mesh, 608 grams, placed in a 2-liter round bottom flask, added with a concentration of 1500 ml of absolute ethanol, refluxed in a water bath for 4.5 hours; filtered, and the filter residue was refluxed 8 times. Combine the ethanol solution, concentrate at 600C under reduced pressure to form an extract; extract the extract with n-butanol at 900C, and cool at 200C to obtain tea saponin solid and n-butanol solution; under reduced pressure, recover n-butanol Alcoholic solution to obtain a mixture containing tea polysaccharides. The above-mentioned tea saponin solid was extracted at 90°C with n-butanol again, cooled at 20°C, and filtered to obtain 72 grams of tea saponin white solid, with a content of 90%; the n-butanol solution was recovered to obtain a mixture containing tea polysaccharides. Combine the mixture containing tea polysaccharides, add (V / V) 99% chemically pure ethanol 1200 ml, let stand for 1 day, filter to obtain 3.1 grams of tea polysac...

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PUM

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Abstract

This invention relates to a method of taking tea oil residue as raw material to extract tea saponin and tea polyose. The preparation process includes: preparation of extract, dissolution of normal butyl alcohol and separating of solid; solid once again dissolve in higher temperature anhydrous normal butyl alcohol, cooling, filtration, gain tea saponin; reclaim normal butyl alcohol solution, gain hybrid containing tea polyose; add hydrous ethanol to the hybrid, the insoluble substance is tea polyose product. This invention has merits of simple craft, low cost and high yield, and low environmental pollution. The product purity is high , tea saponin is white, tea polyose is white.

Description

technical field [0001] The invention belongs to the technical field of food and medicine, and in particular relates to a method for separating tea saponin and tea polysaccharide. Background technique [0002] Scientific research has found that plant polysaccharides have special physiological activities, for example: heparin has anticoagulant effect, chondroitin can prevent vascular sclerosis; lentinan, pachyphyllin, and ganoderma polysaccharide all have significant anticancer activity. Tea polysaccharides are mainly composed of glucose, arabinose, xylose, fucose, ribose, galactose and so on. Modern medical research shows that tea polysaccharide is the main pharmacological component of tea in treating diabetes, and its main pharmacological effects include the following aspects: [0003] (1) Hypoglycemic effect. [0004] Diabetes mellitus is a syndrome characterized by persistent hyperglycemia as its basic biochemical feature. Various reasons lead to insufficient supply of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08C08B37/00
Inventor 杜立春陈建民吴蓓莉
Owner FUDAN UNIV
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