Method and apparatus for inspecting gel chip

Abstract

A method for detecting gel chip includes using deionized water to wash gel chip hybridized with probe, inserting washed gel chip into card slot of chip, placing said card slot into chip washing container for carrying out ultrasonic treatment, setting power of ultrasonic generator to be 20W when 1-10 pieces of gel chips are washed then increasing said power by 10W and making ultrasonic time be 5-30 seconds when a magnitude order of 10 pieces is washed later on, taking out said gel chips, using deionized water to wash them and using nitrogen to blow them to be dried then carrying out scanning on said gel chip.

Description

1. Technical field [0001] The invention belongs to the field of biochip detection, in particular to a method and a device for improving the detection sensitivity of a gel chip. 2. Background technology [0002] Existing technology: In the past decade, chip technology has played a pivotal role in life science research and has also brought profound impact. With the continuous deepening of interdisciplinary research, biochips have become an international research frontier and hotspot. In particular, gel chips with a three-dimensional structure have a strong ability to fix nucleic acids, and polyacrylamide gels and agarose gels have been used in the preparation of three-dimensional gel chips. As a chip that can be used in medical diagnosis, how to improve its detection sensitivity is the key to obtaining high-quality chip data, and the important factors affecting these two indicators are how to effectively remove unhybridized probes on the surface of the gel chip, It is one of...

AI Technical Summary

Problems solved by technology

At present, the method for improving the sensitivity of the chip detection chip is usually to perform various treatments on the chip before the chip is sampled, such as performing hydrophobic or hydrophilic treatment with a silylating reagent, so as to reduce the amount of glass slides in the reaction process. Adsorption of easily combined impurities, but it cannot fundamentally solve the problem of contamination of non-paired probes or impurities after hybridization; another measure is to process the chip after hybridization, such as using 0.1×SSC-0.1% SDS solution, Or use electrophoresis to remove unbound probes or charged impurities, etc. Although these methods can play a certain role in improving the sensitivity of the chip, the first method is for the non-specific binding of probes on the detection target. It is difficult to remove, and although the second method has a certain effect on the removal of non-specifically bound probes and charged impurities, there are still a large part of non-charged impurities that are difficult to remove

[0003] In view of the fact that the chip has been widely used in clinical and scientific research, but the chip, especially the gel chip, still has the limitation of low sensitivity. Therefore, there is an urgent need for a simple and efficient processing method and device for improving sensitivity.

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