Method for preparing microscopic image of holographic digitalized sliced sheet

A technology of microscopic images and production methods, applied in the direction of digital output to display equipment, microscopes, image communication, etc., can solve long-term problems, achieve clarity, optimize consultation operating procedures, increase storage and repeatability Effect

Active Publication Date: 2007-12-26
陈生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this processing method takes a long time, so in order to shorten the processing time, it is necessary to connect two or more hospitals by communication lines and establish a microscope observation system, that is, to use a microscope in a hospital

Method used

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  • Method for preparing microscopic image of holographic digitalized sliced sheet
  • Method for preparing microscopic image of holographic digitalized sliced sheet
  • Method for preparing microscopic image of holographic digitalized sliced sheet

Examples

Experimental program
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Embodiment 1

[0023] Referring to Fig. 1 and Fig. 2, the method for making a microscopic image of a holographic digital slice, the specific steps are as follows:

[0024] A. Use an automatic microscope to collect the microscopic image information of a slice, and control the stage of the automatic microscope through the set control program to carry the slice through the objective lens of the automatic microscope in sequence according to the specified collection range, collection order and step length, The microscopic image units displayed on the objective lens are photographed one by one by a digital image capture device connected to the automatic microscope, and the data conversion is completed to generate serialized multiple microscopic image unit data; The micro image unit data includes at least all pixel information of the microscopic image of the slice, the magnification information of the objective lens, the sequence information of the micro image unit, the coordinate information of the...

Embodiment 2

[0048] Referring to Fig. 3, the present embodiment is an improvement on the basis of Embodiment 1. When the automatic microscope collects microscopic image information of a slice, the used objective lens is a low power objective lens (the magnification of the objective lens is 4, referred to as low magnification objective lens), according to the steps described in Embodiment 1, collect and make, the microscopic image of the generated holographic digital slice is a low magnification image, adopt the JPEG algorithm to compress the microscopic image, and classify and divide the microscopic image according to the compression format Blocks are stored in an image file. The low magnification objective lens refers to 4 times or 10 times. This method collects fewer images, has faster image processing speed, saves computer resources, and is suitable for fast panorama browsing.

Embodiment 3

[0050] Referring to Fig. 4, the present embodiment is an improvement on the basis of Embodiment 1. When the automatic microscope collects microscopic image information of a slice, the used objective lens is a high-magnification objective lens (the magnification of the objective lens is 40, referred to as High magnification objective lens), collect and make according to the steps described in Embodiment 1, the microscopic image of the generated holographic digitized slice is a high magnification image, adopt the JPEG algorithm to compress the microscopic image, and classify and divide the microscopic image according to the compression format Blocks are stored in an image file. The grading refers to converting the high-magnification microscopic image of the holographic digitized slice into microscopic images of several magnification levels, for example: 4 times, 10 times, 20 times, 40 times. The microscopic images of the holographic digitized slices of the four magnification lev...

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Abstract

A method for preparing microscopic image of hologram digitalized slab includes using automatic microscope to collect microscopic image information of a slab to generate sequenced multiple microscopic image unit data, carrying out matching treatment on relevant position between each two adjacent microscopic image units to obtain position data of each microscopic image unit, carrying out fusing treatment on overlapped portion between each two adjacent microscopic image units according to visual habits of human eyes for generating microscopic image of hologram digitalized slab then compressing said image blocking-store it in image file.

Description

technical field [0001] The invention relates to a method for making a microscopic image of a holographic digital slice, which collects the microscopic image of the slice based on an automatic microscope and a digital image acquisition device, and performs matching and fusion processing on the microscopic image through a computer system and image processing software. Generate holographic digitized microscopic images of slices. Background technique [0002] In the prior art, when using a microscope for pathological observation, the usual method is that the user uses a microscope with a low magnification to conduct preliminary observations for initial diagnosis, and then uses a microscope with a high magnification for detailed observation. In hospitals in remote areas without pathologists, accurate judgments cannot be made on specimens. Therefore, it is often necessary to send the specimen to a large hospital and entrust a consultation to obtain an accurate judgment. However,...

Claims

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Application Information

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IPC IPC(8): G02B21/00G02B21/36G06F3/14H04N5/225
Inventor 陈生王磊阚淑丽朱甘甫
Owner 陈生
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