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Method of separating and extracting natural base from coptis chinensis with latex membrane

An emulsion liquid film and alkaloid technology, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve the problems of cumbersome operation, high consumption of raw materials, and inapplicability, and achieve simple operation and fast extraction process Effect

Inactive Publication Date: 2008-01-23
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is cumbersome, time-consuming, and consumes a lot of raw materials.
Emulsion liquid membrane method is a simple, fast, efficient, highly selective, economical and energy-saving new separation and extraction technology, which has been applied in the fields of environment, chemical production, hydrometallurgy, etc., but has not been applied to the extraction of total alkaloids in Chinese herbal medicines. extraction, and the film solvents of the existing emulsion liquid film are usually toluene, cyclohexane, dichloromethane and kerosene; these solvents have certain toxicity, and are not suitable for the use of Chinese herbal medicines for humans and animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Preparation of Coptis chinensis extract: Weigh 1.0 g of Coptidis rhizome powder, add 50 mL of 0.1 mol / L HCl, ultrasonically extract for 60 min, centrifuge for 10 min in a centrifuge at a speed of 4000 r / min, take out the filtrate, and add 50 mL to the filter residue 0.1mol / L HCl, ultrasonically extracted for 60min, then centrifuged for 10min, and the filtrates were combined. Dilute the filtrate to 900 mL with 0.1 mol / L HCl, filter, adjust the pH to 4 with saturated sodium hydroxide, and set aside.

[0016] (2) Preparation of emulsion: take 37mL of film solvent edible oil, 7mL of surfactant total amount, wherein Span80 is 1mL, n-decyl alcohol is 6mL, phospholipid carrier 6mL, mix the three, take this mixture 20mL Add 10 mL of 0.1 mol / L HCl solution at a volume ratio of 2:1, and stir for 2 min at a speed of 9000 rpm to obtain a stable W / O emulsion.

[0017] (3) Separation and extraction of alkaloids: Add the Coptidis rhizome hydrochloride extract and emulsion with pH...

Embodiment 2

[0029] (1) Preparation of Coptidis Rhizoma Extract: Weigh 2 kg of Coptidis Rhizoma Extract powder, add 120 L of 0.1mol / L HCl, ultrasonically extract for 70 min, centrifuge for 10 min in a centrifuge at a speed of 4000 r / min, take out the filtrate, and pour it into the filter residue Add 120 L of 0.1 mol / L HCl, ultrasonically extract for 70 min, then centrifuge for 10 min, and combine the filtrates. Dilute the filtrate to 2400L with 0.1mol / L HCl, filter, adjust pH=4 with saturated sodium hydroxide, and set aside;

[0030] (2) Preparation of emulsion: get film solvent edible oil 124L, surfactant total amount is 36L, wherein Span80 is 14L, n-decyl alcohol is 22L, phospholipid carrier 40L, three are mixed, then this mixture is mixed with Add 0.7 mol / L HCl solution into the milk maker at a volume ratio of 4:1, and stir for 15 minutes at a speed of 10,000 rpm to obtain a stable W / O emulsion.

[0031] (3) Separation and extraction of alkaloids: add Coptidis rhizome hydrochloric acid...

Embodiment 3

[0043] (1) Preparation of Coptis chinensis extract: Weigh 1.0 g of Coptidis rhizome powder, add 30 mL of 0.1 mol / L HCl, ultrasonically extract for 30 min, centrifuge for 10 min in a centrifuge at a speed of 4000 r / min, take out the filtrate, and add 30 mL to the filter residue 0.1mol / L HCl, ultrasonically extracted for 30min, then centrifuged for 10min, and the filtrates were combined. Dilute the filtrate to 500 mL with 0.1 mol / L HCl, filter, adjust the pH to 4 with saturated sodium hydroxide, and set aside.

[0044] (2) Preparation of emulsion: Take 42mL of film solvent edible oil, 3.5mL of total surfactant, wherein Span80 is 0.5mL, n-decyl alcohol is 3mL, phospholipid carrier 4.5mL, and the three are mixed. 20 mL of this mixture was added with 20 mL of 0.4 mol / L HCl solution at a volume ratio of 1:1, and stirred at a speed of 8000 rpm for 1 min to obtain a stable W / O emulsion.

[0045](3) Separation and extraction of alkaloids: add the Coptidis rhizome hydrochloric acid ext...

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PUM

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Abstract

The invention discloses a method for extracting alkaloid in Chinese goldthread coptis through separating emulsion liquid membrane. The emulsion is prepared by taking cooking oil as the membrane solvent, the Span 80 or the mixture of the Span 80 and the cosurfactant as the surface active agent and the phosphate ester as the carrier. Adding the Chinese goldthread coptis hydrochloric acid extraction liquid or vitriol extraction liquid with the pH value equals to 4 and the emulsion with the volume ratio between water and the emulsion as (50:5 to 50:20); stiring with low speed of 200 times per mins to 300 times per mins for extracting for 5 to 15 min; separating out the oil layer after breaking the emulsion through heating in water and the water obtained is just the solution of the total alkaloid in the Chinese goldthread coptis. The invention, by taking the cooking oil as the membrane solvent, resolves the difficulty that the organic solvent used for the existing emulsion membrane is venomous and is unsuitable being used for human and animal after extracted the Chinese medicinal herb, has the advantages of speedy extraction, high efficiency and simple operation, which is applied for producing, developing and inspecting the Chinese goldthread coptis medicine and veterinary medicine.

Description

technical field [0001] The invention relates to a method for extracting alkaloids in coptis chinensis by emulsion liquid membrane separation and applicable to medicinal material production and medicinal analysis and testing. Background technique [0002] Coptidis Rhizoma Coptidis is a kind of traditional Chinese medicine with a wide range of uses. The dried rhizome is used for medicinal purposes. The main medicinal ingredients are berberine, jatrorrhizine, palmatine and other alkaloids. They have similar structures and are often collectively referred to as coptis alkaloids. The commonly used extraction method is reflux extraction, such as ethanol water reflux extraction or water reflux extraction. The finished product obtained by this process contains more impurities, and the purity of the finished product needs to be improved by recrystallization, but recrystallization inevitably leads to the loss of the total alkaloids of Coptis chinensis. 02103880.5 discloses "an extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/718
Inventor 马铭欧阳丽何鼎胜陈波姚守拙
Owner HUNAN NORMAL UNIVERSITY
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