Unlock instant, AI-driven research and patent intelligence for your innovation.
Application of berberine and its derivates in protein fluorescent detecting
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A fluorescence detection and protein technology, applied in the field of protein fluorescence detection, can solve the problem of no protein gel fluorescence staining, and achieve the effect of ensuring high selectivity and sensitivity, good reproducibility and little environmental pollution
Inactive Publication Date: 2012-05-02
WENZHOU MEDICAL UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
So far, there are no reports of its use for fluorescent staining on protein gels after electrophoresis
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0027] The dyeing effect comparison of embodiment 1 different dyestuffs
[0028] The protein sample in this example is SDS-6H standard protein (Sigma).
[0029] Berberine protein fluorescent staining method:
[0030] 1) Place the protein sample gel after SDS-PAGE electrophoresis in a fixative for 5 minutes, discard the fixative, the fixative is 40% ethanol and 10% aqueous acetic acid by volume, and rinse with deionized water 3min;
[0031] 2) Add fluorescent staining solution for staining for 5 minutes, rinse with deionized water for 3 minutes, the staining solution is an aqueous solution containing 0.0005% (w / v) berberine dye and 2% acetic acid, and then rinse with deionized water for 0.5 minutes;
[0032] 3) Detection. Place the stained gel on a transilluminator, transmit at 312nm, and record the image.
[0033] Carry out protein staining with hydrochloride, sulfate, bisulfate, nitrate, tannate, hydroiodide of berberine respectively according to the above-mentioned metho...
Embodiment 2E
[0043] Example 2E. coliBL21 total protein two-dimensional electrophoresis staining
[0044] Take the E.coli BL21 bacterial liquid, collect the bacterial cells by centrifugation at 12000 rpm, and add 0.02mol / L phosphate buffer (pH7.0) to resuspend the bacterial cells. Cells were disrupted using a sonicator. Centrifuge at 18000rpm for 30 minutes, collect the supernatant, and obtain the total protein of E.coli. After the above-mentioned total protein is subjected to two-dimensional gel electrophoresis, it is stained with berberine according to the method of Example 1. The result is as follows figure 2 shown. Depend on figure 2 It can be seen that the visualized protein spots of this staining method and sYPR0Red staining method are basically consistent, and this method is faster and more sensitive.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
melting point
aaaaa
aaaaa
wavelength
aaaaa
aaaaa
Login to View More
Abstract
The invention relates to a protein fluorescence detection technology, in particular to the application of the berberine and the derivatives in the protein fluorescence detection. The invention also provides a method to apply the berberine to the protein fluorescence gel detection, and the method comprises the steps of: fixing the electrophoresis gel of the protein sample in the stationary liquid,washing by the deionized water; dyeing, washing by the deionized water; detection. The invention has the advantages of high sensitive, simple and rapid operation, good reproducibility, good dyeing background, good linear relationship, good reversibility, good compatibility, safe using and low cost, etc.
Description
technical field [0001] The invention relates to protein fluorescence detection technology, in particular to a new fluorescent dye of protein. Background technique [0002] In the field of life science research, it is inseparable from the research on protein and DNA, the basic substances of life, and also inseparable from the detection technology of protein. Especially in recent years, with the rapid development of genomics and proteomics, we have higher requirements for protein separation and detection technology. [0003] There are many protein detection methods, including turbidimetry, Kjeldahl method, absorbance photometry, fluorescence photometry, and the newly developed resonance Rayleigh scattering method, high performance liquid chromatography, and capillary electrophoresis analysis. Fluorescence photometry is currently the most researched, most widely used, easy-to-operate, and highly sensitive detection method. [0004] Berberine is an isoquinoline alkaloid with t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More