Rotary type multichannel fluorescence excitation method based on input-output optical fiber

Abstract

The invention discloses a rotary type multichannel fluorescence excitation device and a method based on inlet and outlet optical fibre, belonging to the photoelectric detection field. The device sequentially consists of a light source (1), an incidence coupling lens (3), inlet optical fibre (4), outlet optical fibre (6) and an outlet coupling lens (7), and is characterized in that: the inlet optical fibre is a group of multichannel inlet optical fibre, and the outlet optical fiber is a group of multichannel outlet optical fibre; the rear of the outlet coupling lens also comprises a releasing optical filter or a prism device (8) and a photoelectric detector (9) connected with a computer (10); the light emitted by the outlet optical fibre becomes parallel light through the outlet coupling lens, and the light passing through the releasing optical filter or the prism device is all projected on a rotary disk to drive a motor so as to drive the disk to rotate; a hole is opened in a positionwhere the light is projected on the disk, after passing through the hole, the projection light is vertically injected on the photoelectric detector which is connected with the computer, thus completing a detection; the rotation of the disk sequentially leads the light of each channel optical fibre to pass through the hole for detecting so as to realize a multichannel detection. The mechatronics ofthe invention has simple structure, fast speed and high detection precision.

Description

technical field

[0001] The invention relates to a rotary multi-channel excitation fluorescence device and method based on input and output optical fibers, and belongs to the technical field of photoelectric detection in biomedicine. Background technique

[0002] Because the external light source stimulates the object to be detected to induce fluorescence detection, it has very little impact on organisms, animals and human bodies. More importantly, the dynamic range and sensitivity of fluorescence detection are getting higher and higher, approaching or catching up with radioactive detection, so fluorescence detection It is widely used in life sciences, medical research and practical applications, especially in the field of real-time detection of living organisms, animals and human bodies.

[0003] In the classic multi-channel excitation-induced fluorescence device and method, one input optical fiber and another output optical fiber are used to transmit light, that is, one inp...

Examples

Embodiment

[0018] For such as figure 1 In the single-channel detection device shown, the light emitted by the light source 1 becomes monochromatic light through the excitation light filter 2, and then converges through the incident coupling lens 3, and then couples into an optical fiber in a group of multi-channel input optical fibers 4. After the colored light comes out from the input optical fiber 4, it irradiates the sample 5 in the reaction cell, and the sample 5 is excited to emit fluorescence; the excited fluorescence is directly coupled into an optical fiber in a group of multiple output optical fibers 6, and the fluorescent light from the output optical fiber 6 passes through The output coupling lens 7 becomes parallel light, and all the narrow-band fluorescence filtered by the release filter 8 is projected onto a rotatable disk, and the drive motor drives the disk to rotate; a hole is opened at the place where the fluorescence is projected to the disk, and the projected fluoresce...

technical field

[0001] The invention relates to a rotary multi-channel excitation fluorescence device and method based on input and output optical fibers, and belongs to the technical field of photoelectric detection in biomedicine. Background technique

[0002] Because the external light source stimulates the object to be detected to induce fluorescence detection, it has very little impact on organisms, animals and human bodies. More importantly, the dynamic range and sensitivity of fluorescence detection are getting higher and higher, approaching or catching up with radioactive detection, so fluorescence detection It is widely used in life sciences, medical research and practical applications, especially in the field of real-time detection of living organisms, animals and human bodies.

[0003] In the classic multi-channel excitation-induced fluorescence device and method, one input optical fiber and another output optical fiber are used to transmit light, that is, one inp...

Examples

Embodiment

[0018] For such as figure 1 In the single-channel detection device shown, the light emitted by the light source 1 becomes monochromatic light through the excitation light filter 2, and then converges through the incident coupling lens 3, and then couples into an optical fiber in a group of multi-channel input optical fibers 4. After the colored light comes out from the input optical fiber 4, it irradiates the sample 5 in the reaction cell, and the sample 5 is excited to emit fluorescence; the excited fluorescence is directly coupled into an optical fiber in a group of multiple output optical fibers 6, and the fluorescent light from the output optical fiber 6 passes through The output coupling lens 7 becomes parallel light, and all the narrow-band fluorescence filtered by the release filter 8 is projected onto a rotatable disk, and the drive motor drives the disk to rotate; a hole is opened at the place where the fluorescence is projected to the disk, and the projected fluoresce...

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