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Method for transferring cells

A cell, to-be-migrated technology, applied in the field of migrating cells, to achieve the effect of convenient operation and easy access

Inactive Publication Date: 2010-12-22
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, cell migration experiments mainly use microporous filter membrane culture chambers and double-chamber cell co-culture methods for cell migration, and it is impossible to study the migrated cells and their migration rates from cell clone balls or tissue blocks

Method used

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  • Method for transferring cells
  • Method for transferring cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] 1. Select hair with a diameter of 5 microns and a length of 0.1 mm as the migrating silk, wash, sterilize, and dry the selected migrating silk for later use;

[0027] 2. Obtain cell cloning spheres by cell cloning sphere culture. Cell cloning sphere cultivation is already a commonly used method. The specific cultivation steps will not be described here. Put the obtained cell cloning spheres into Culture well plate, for some cells, growth factors need to be added to the culture medium, and serum-free, the glioma cell line U251 culture medium used in this example;

[0028] 3. Operate under a microscope, insert one end of the migration wire into the prepared cell cloning ball, and after 24 hours, some cells can be found to migrate out of the cell cloning ball along the migration wire.

[0029] Such as figure 1 In the photomicrograph of Example 1 shown, it can be seen that many cells migrate out from the suspended cell clone ball along the migration filament, and the migr...

example 2

[0031] 1. Select a feather with a diameter of 20 microns and a length of 100 microns as the migration silk, wash, sterilize, and dry the selected migration silk for subsequent use;

[0032] 2. Operate under a microscope, place the migrating silk on the cloning ball, after a period of time, you can find that the cloning ball cells will partially wrap the migrating silk placed on the cloning ball, and then after a certain period of time (for example, 24 hours) Afterwards, some cells can be found to migrate out of the tissue fragment along the migratory filament.

[0033] Such as figure 2 In the photomicrograph of Example 2 shown, it can be seen that although some cells migrate out from the suspended tissue fragments along the migration filament due to the thicker migration filament, they are not the same as figure 1 Compared to the state shown, the migration is less effective.

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Abstract

The invention discloses a method of migrating cells, comprising the following steps: the step of preparing a migration wire: fine hair or feather or nonpoisonous innocent polymer material fiber is chosen as the migration wire, and is cleaned, sterilized and dried to standby; the step of preparing the migrated cell tissues: cell clones are gotten by the culture method of a cell clone, or a fresh tissue block is sheared into small blocks as the migrated cell tissues which are put into a culture hole plate with culture medium; the migrating step: under a microscope, one end of the migration wireis inserted into the migrated cell tissues or is put on the migrated cell tissues, the migration wire floats in the culture medium, and in a time cells will migrate out of the migrated cell tissues along the migration wire. The invention has the advantages: adopting fine hair or feather as the migration wire enables the cells to migrate efficiently along the migration wire, the materials of the migration wire are easily gotten, and the operation is convenient; the invention can be widely used in such fields as cell theory study, oncology and new drug development.

Description

technical field [0001] The invention relates to the field of cell or tissue culture, in particular to a method for migrating cells. Background technique [0002] The study of cell migration is an area involved in biological, medical and pharmaceutical research, especially in the fields of tumor and stem cell biology. At present, cell migration experiments mainly use microporous filter membrane culture chambers and double-chamber cell co-culture methods for cell migration, and it is impossible to study the migrated cells and their migration rates from cell clone balls or tissue blocks. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for migrating cells, which is easy to operate and can facilitate the study of migrating cells and their migration rate. [0004] In order to solve the above-mentioned technical problems, the present invention adopts the following technical scheme: a method for migrating ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00
Inventor 金刚
Owner SHENZHEN POLYTECHNIC