Method of fabricating microarray
一种基板、探针单元的技术，应用在生物化学设备和方法、微生物的测定/检验、用于材料和表面科学的纳米技术等方向，能够解决反应产率减小、除去光敏感保护基团难等问题
Inactive Publication Date: 2008-07-16
SAMSUNG ELECTRONICS CO LTD
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Additionally, as microarrays become highly integrated, the spacing between immobilized functional groups decreases, thereby making removal of photosensitive protecting groups more difficult and resulting in reduced reaction yields
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[0021] The invention may be embodied in many different forms and should not be considered limited to the exemplary embodiments set forth herein.
[0022] Thus, in some embodiments, well known process steps are typically not described in detail in order not to unnecessarily obscure the description of the present invention.
[0023] Note that any and all examples, or exemplary terminology used herein, are intended to better illuminate the invention only and are not intended to be limiting of the scope of exemplary embodiments of the invention unless otherwise stated. The terms indefinite and definite articles and similar signs used in the context of describing the invention (especially in the context of the following claims) are to be construed as covering both the singular and the plural unless otherwise stated herein or clearly contradicted by context. The term "comprising" is to be construed as an open term (i.e., meaning "including but not limited to") to mean some and all c...
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A method of manufacturing a microarray includes providing a substrate having a surface that is immobilized with a functional group protected with an acid-labile protecting group and capable of coupling with an oligomer probe, providing a photoacid generator onto the substrate, disposing on the substrate an imprint template comprising a convex region and a plurality of concave regions surrounding the convex region so that the convex region contacts with or is adjacent to an upper surface of the substrate to define a plurality of reaction zones by the upper surface of the substrate and the convex region and the concave regions of the imprint template, exposing one or more of the reaction zones to light so that an acid is generated by the photoacid generator in the one or more exposed reaction zones and a functional group in the one or more exposed reaction zones is deprotected by the acid, and providing an oligomer probe onto the substrate so that the oligomer probe couples with the deprotected functional group.
Description
technical field [0001] The present disclosure relates to a method of manufacturing a microarray, and more particularly, to a method of manufacturing a microarray with an improved reaction yield. Background technique [0002] With the progress of genome projects, the genome nucleotide sequences of various organisms have been published. As a result, interest in biopolymer microchips, especially microarrays, has increased. Microarrays are widely used for example in gene expression profiling, genotyping by detection of mutations or polymorphisms such as single nucleotide polymorphisms (SNP), protein or peptide assays, possible drug screening, development of new drugs and Tools for preparation etc. [0003] Widely available microarrays can be removed by, for example, exposing predetermined regions of a substrate containing functional groups protected with light-sensitive protecting groups, exposing the functional groups in the predetermined regions, and then performing in situ ...
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IPC IPC(8): G01N21/00G01N33/48C12Q1/68
CPCB01J19/0046B01J2219/00382B01J2219/00432B01J2219/00527B01J2219/00585B01J2219/00596B01J2219/00605B01J2219/00659B01J2219/00711B01J2219/00722B82Y30/00C40B50/18B01J2219/00351
Inventor 夏政焕池圣敏金京善金媛善
Owner SAMSUNG ELECTRONICS CO LTD