Fluorescence detection method for DNA and kit thereof

Abstract

The invention discloses a DNA fluorescent detection process and its agent box. In the method, target direction DNA is captured through cross-fertilizing between DNA after the capturing detecting probe is coupled to the magnetic particle surface, another nucleotide sequence of target direction DNA is cross-fertilized with semaphore detecting probe modified by luciferin to form sandwich filler structure of magnetic particle-target direction DNA-fluorescence labeling semaphore detecting probe marked, meanwhile competitiveness detecting probe of above capture detecting probe or semaphore detecting probe is added to combine with target direction DNA, finally, water-solubility conjugate high molecule with semaphore multiply function is added to amplify and detect the signal in order to detect the target direction DNA. The invention can improve greatly the selectivity and sensitivity of gene and gene mutation detection without amplifying and increasing nucleic acid and with quick and easy operation, has important meaning in biological detection, morbid early diagnosis and treatment.

Description

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AI Technical Summary

Benefits of technology

This patented technology involves adding specific chemicals called fluorochromic dyes onto certain parts or molecules that have been labeled on them during manufacturing processes for making medicine (such as drugs). These special compounds help distinguish different types of cells from each other based upon their unique properties like shape, size, coloring pattern, etc., allowing researchers to study how these materials behave under various conditions when placed into living organisms. By doing this they may be useful tools for medical purposes including diagnosing disease states earlier than traditional methods involving tissue samples taken up twice over several months ago.

Problems solved by technology

Technologies aiming at developing highly sensitive techniques for identifying tiny amounts of cancer causing changes caused by inherited differences have been developed over recent years due to advancements made during medical treatments like chemotherapy drugs and radiation therapies. These technics include nucleic acid testing, molecular diagnoses, bioinformaticians, immunology experts, and others involved in these applications. Overall, there is a technical problem addressed through this patented technique called qPCR, where precise quantitative measurement of certain types of genomic variations becomes crucial because they play key roles in various aspects including drug discovery and personalized medicine strategies.

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