Ecological cultivation method for spire morel
A kind of peak Morchella, cultivation method technology, is applied in the peak Morchella ecological cultivation field, can solve the problems such as unfavorable forest resource protection, high cost, no large-scale promotion, etc., reach to improve forest coverage, yield improvement, promotion The effect of sustainable development
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Embodiment 1
[0016] 1. Preparation of Populus round-leaf: Take Populus round-leaf cuttings and propagate seedlings free of diseases and insect pests for 5 months,
[0017] 2. Preparation of Morchella apex: 20g of glucose, 4g of peptone, MgSO 4 0.05g, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, H 2 O 1000ml ratio to prepare the culture medium. The culture medium was sterilized at 121° C. for 30 minutes, and after cooling, it was inserted into the mother species of Morchella apicula, and fermented and cultivated at 20° C. for 30 days for use.
[0018] 3. Mechanical damage and disinfection of the roots of poplar seedlings: cut off the fibrous roots of poplar, and perform artificial mechanical damage to the remaining roots. The damaged area is about 50%, and put the roots of poplar into 50ppm potassium permanganate solution After soaking in water for 5 minutes, rinse with water twice.
[0019] 4. Inoculation: Soak the roots of Populus musculus seedlings that have been mechanically damaged and dis...
Embodiment 2
[0023] 1. Preparation of Populus round-leaf: Propagate seedlings of Populus round-leaf with no pests and diseases for 2 months.
[0024] 2. Preparation of Morchella apicaleum bacteria agent: the fermentation medium is PDA medium, the medium is sterilized at 121°C for 30 minutes, after cooling, it is inserted into the mother species of Morchella apexus, and fermented and cultivated at 25°C for 20 days spare.
[0025] 3. Mechanical treatment and disinfection of the roots of Populus rotifera: cut off the fibrous roots of Populus rotifera, and artificially mechanically damage the remaining roots. The damaged area is about 30%, and put the roots of Populus rotifera into 10ppm potassium permanganate solution After soaking for 10 minutes, rinse with clean water 3 times.
[0026] 4. Inoculation: Soak the roots of poplar seedlings that have been mechanically damaged and disinfected with the liquid strain of Morel apex. It is advisable to cover all the injured roots, and the roots that...
Embodiment 3
[0030] 1. Preparation of Populus round-leaf: take Poplar round-leaf seeds and propagate seedlings free from diseases and insect pests for 10 months,
[0031] 2. Preparation of Morchella apicaleum strain: the fermentation medium is PDA medium, and the medium is sterilized at 121°C for 30 minutes. After cooling, it is inserted into the mother species of Morchella apexus, and fermented and cultivated at 25°C for 30 days. spare.
[0032] 3. Mechanical treatment and disinfection of the roots of Populus rotifera: cut off the fibrous roots of Populus rotifera, and perform artificial mechanical damage to the remaining roots. The injured area is about 70%, and put the roots of Populus rotifera into 60ppm potassium permanganate solution After soaking for 3 minutes, rinse with clean water 3 times.
[0033] 4. Inoculation: the roots of poplar seedlings treated with root mechanical damage and disinfection were divided into small pieces with a diameter of 0.5-3cm with solid bacteria before...
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