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Coupling process of marsh gas fermentation and culture of autotrophic freshwater microalgae

A technology for biogas fermentation and microalgae cultivation is applied in the fields of biogas fermentation and autotrophic freshwater microalgae cultivation, which can solve the problems of high cost, waste gas and waste liquid, etc., and achieve the effects of reducing production cost, improving environment and increasing economy.

Inactive Publication Date: 2010-07-28
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aiming at the high cost of autotrophic freshwater microalgae culture medium and the waste gas and waste liquid of biogas fermentation, a method of constructing a coupled culture system for the two is proposed

Method used

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  • Coupling process of marsh gas fermentation and culture of autotrophic freshwater microalgae
  • Coupling process of marsh gas fermentation and culture of autotrophic freshwater microalgae

Examples

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Effect test

Embodiment 1

[0027] The microalgae Neochloris oleoabundans (purchased from the American UTEX Algae Collection Center, UTEX#1185) in this example is an autotrophic freshwater algae, and the total oil content in the BG11 medium can reach more than 45% of the dry biomass, which is an oil-rich microalgae. Using biogas and biogas slurry as the whole nutrient source of Neochloris oleoabundans, the algal cells were cultivated in a small pipeline photobioreactor at room temperature. The algae have been experimentally confirmed to survive under these conditions. The steps are as follows:

[0028] A: Biogas fermentation - mix human and animal manure, domestic garbage and other waste biomass with water at a ratio of 1:19, insert 30% anaerobic activated sludge, and anaerobically ferment at 35°C for 25 days to obtain biogas, biogas slurry, and biogas It is introduced into the photobioreactor through the pipeline, and the biogas slurry is used as the medium after treatment.

[0029] B: Treatment of b...

Embodiment 2

[0035] Using the photoautotrophic Ankistrodesmus pseudobraunii (purchased from the American UTEX Algae Collection Center, UTEX#LB 1380) as the microalgae species, using biogas and biogas slurry as the entire nutrient source of the algae, a small pipeline photobiological process was carried out at room temperature. Algae cells are grown in the reactor. The algae has been proved by experiments that the biogas slurry can be used as the only medium to survive, and it is an oil-rich microalgae. The steps are as follows:

[0036] A: Biogas fermentation - mix waste biomass such as water hyacinth, human and animal manure, and domestic waste with water at a ratio of 1:20, insert anaerobic activated sludge at a weight ratio of 10%, and anaerobically ferment at 35°C for 20 days. Obtain biogas and biogas slurry. The biogas is introduced into the photobioreactor through the pipeline, and the biogas slurry is used as the medium after treatment.

[0037]B: Treatment of biogas slurry - tak...

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Abstract

The invention belonging to the environmental protection and new technique energy source field discloses a coupling method for biogas fermentation and autotrophy type fresh water micro-algae culture. The invention is characterized by adopting the method of simultaneously carrying out biogas fermentation and autotrophy type fresh water micro-algae culture including biogas fermentation, marsh liquidprocessing, autotrophy type fresh water micro-algae inoculation and biogas introduction, control of autotrophy type fresh water micro-algae culture, and harvest of autotrophy type fresh water micro-algae culture. The invention adopts the coupling method of biogas fermentation process and phototrophy type fresh water micro-algae culture. The marsh liquid is taken as the culture medium of autotrophy fresh water micro-algae, and the CO2 in the biogas is taken as the CO2source needed by autotrophy type fresh water micro-algae culture, thereby greatly reducing the production cost of autotrophy type fresh water micro-algae and solving the problems of biogas purification and marsh liquid aftertreatment.

Description

Technical field: [0001] The invention relates to a method for coupling biogas slurry utilization and biogas purification with autotrophic freshwater microalgae cultivation, which is specially used for biogas fermentation and autotrophic freshwater microalgae cultivation. Background technique: [0002] Autotrophic microalgae can fix CO under photosynthesis 2 , and use inorganic nitrogen, phosphorus and other inorganic salts as nutrient sources, it has the characteristics of high photosynthetic efficiency, strong environmental adaptability, short growth cycle and high biomass production, and is an ideal biomass energy material. The comprehensive development and utilization of microalgae, especially oil-rich microalgae with high oil content, has become an important measure for many countries to increase energy and save energy, turn harm into benefit and protect the environment. Various inorganic salts of autotrophic microalgae account for more than 60% of its total cost. If th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02C12N1/12C12R1/89
CPCY02E50/343Y02E50/30
Inventor 欧阳平凯韦萍何若平李环周华应汉杰黄和
Owner NANJING TECH UNIV
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