Method for applying pretreatment of amylase to swine waste marsh gas fermentation

Abstract

The invention relates to a method for producing methane by utilizing enzyme to process organisms. The method includes the steps that pig manure is weighted and fermentation liquid is prepared according to the concentration of the total content of solids (TS) of the pig manure; Alpha-amylase or Gamma-amylase is added to the prepared fermentation liquid for processing the liquid; the processed fermentation liquid is mixed with inoculum for the fermentation of the methane. The method adopts amylase for the pre-processing of the fermentation of the pig manure and the result shows that the degradation rate of the TS and the VS of the pig manure, the methane yield factor in the maximum volume, the accumulated methane yield and the potential of the raw materials for methane yield have remarkable growth compared with a comparison group, wherein, the processing effect of disaccharidase is better than that of the Alpha-amylase while the processing effect of the Alpha-amylase is better than that of the Gamma-amylase.

Description

technical field [0001] The invention belongs to a biogas fermentation method which uses enzymes to process organic matter in an anaerobic environment to generate biogas. Background technique [0002] Biogas fermentation involves the synergistic action of a variety of microorganisms to jointly degrade complex organic matter, which can be divided into three stages: hydrolysis, hydrogen production, acid production, and methane production (Zhang Wudi, Liu Shiqing, Multifunctionality of anaerobic digestion process[J], Tianjin Microbiology, 1995(3):24-26). When the raw material used in biogas fermentation is macromolecular complex organic matter, the hydrolysis stage will be the main limiting stage of the whole process (Zhang Jishi, Sun Kewei, Xu Jing, Research on the influence of lysis treatment of municipal organic solid waste and its heap anaerobic digestion[ J], Ecological Environment, 2006, 15(4): 862-865). Therefore, before biogas fermentation, pretreatment of macromolecul...

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Problems solved by technology

However, none of these studies and experiments involve the treatment of pig manure with hydrolytic enzymes before biogas fermentation to degrade

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