Test paper and method for checking mycobacteria tuberculosis and non-mycobacteria tuberculosis nucleic acid amplifying products

Abstract

The invention provides a test paper of nucleic acid amplification product for detecting tubercle mycobacterium (TB) and Nontuberculous mycobacteria (NTM), comprising (a) (avidin-gold release region) and (b) test belt. The invention further provides a method of detecting TB and NTM, comprising (a) amplifying the sample DNA of primer having mark; (b) mixing the amplified DNA product by electrophoretic buffer; (c) dipping the test paper in the mixture; and (d) flowing the mixture in the reaction area of the paper.

Description

technical field The invention relates to a test paper and a method for quickly identifying whether there are amplified DNA products of mycobacterium tuberculosis and non-tuberculosis mycobacterium. Background technique Mycobacterium tuberculosis is a bacterium that causes tuberculosis (Tuberculosis, TB). Mycobacterium tuberculosis mostly attacks the respiratory system, but can also infect other organs, tissues, etc. of the body. Mycobacterium tuberculosis is spread through the air when its carriers cough, sneeze or talk. In 2004, statistics showed that 14.6 million people were chronically ill, 8.9 million were new cases, and 1.6 million died. Most of these people were in developing countries. However, due to the abuse of immunosuppressive drugs or AIDS infection, more and more people in developed countries suffer from tuberculosis. Nontuberculous mycobacteria (NTM) does not cause what we call pulmonary tuberculosis symptoms, and infection with nontuberculous mycobacteria...

AI Technical Summary

Problems solved by technology

Diagnosis is only found to need revision when bacterial cultures are performed for approximately six weeks, leading to confusion for patients or physicians during this period

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