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Preparation of carcinoma antigen 125 EUSA detection reagent kit

A detection kit and immunoadsorption technology, applied in the field of preparation of cancer antigen 125 enzyme-linked immunosorbent detection kit

Inactive Publication Date: 2008-12-03
丁恩雨 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the specificity and sensitivity of these products need to be further improved [4]

Method used

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Experimental program
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Embodiment Construction

[0006] First, synthesize a polypeptide according to the above amino acid sequence 1, combine it with a carrier protein (such as KLH) to prepare a CA125 monoclonal antibody, and coat it in the microwells of the reaction plate to make a solid-phase antibody.

[0007] Secondly, synthesize the polypeptide according to the above amino acid sequence 2, combine with carrier protein (such as KLH), and mix with antigen adjuvant, prepare rabbit anti-CA125 polyclonal antibody by immunizing rabbits, and then combine with horseradish peroxidase (HRP) to prepare Enzyme-labeled antibody was obtained. During the detection, the sample can be combined with the solid-phase CA125 monoclonal antibody and the enzyme-labeled rabbit anti-CA125 polyclonal antibody at the same time, and the rest is carried out according to the conventional method. After the substrate is added, the degree of color development is positively correlated with the concentration of CA125 in the sample. Compared with the stan...

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Abstract

The invention relates to a cancer antigen 125 (CA125) enzyme-linked immune adsorption detection kit, which can directly detect the concentration of the CA125 in human serum rapidly. The kit has the main technical characteristics that a monoclonal antibody is prepared by selecting a specific amino acid sequence in a CA125 exon 1; a rabbit-anti CA125 polyclonal antibody is prepared by selecting a specific amino acid sequence in a CA125 exon 3. When the detection is performed, samples can be combined with the solid-phase monoclonal antibody and the enzyme labeled rabbit-anti CA125 polyclonal antibody. The increase of CA125 level in the serum often occurs in case of ovarian cancer, endometritis and other cancers. The detection kit can be used for detecting ovarian cancer, etc. and assessing the treatment prognosis effect.

Description

Technical field [0001] The patent of the present invention relates to the preparation of an enzyme-linked immunosorbent assay (ELISA) detection kit capable of quantitatively measuring the concentration of cancer antigen 125 (CA125) in human serum or plasma. Background technique [0002] Ovarian cancer is the eighth most common cancer among women. Currently, there is still no standard screening method to detect ovarian cancer [1， 2] . CA125 in serum is a high molecular weight glycoprotein [3] , is a surface antigen associated with epidermal ovarian cancer. Studies have shown that elevated serum CA125 levels are seen in severe endometritis and ovarian cancer. More than 60% of women with ovarian cancer have serum CA125 concentrations higher than 35 units / ml. The change of serum CA125 concentration can also be used for postoperative monitoring and judgment of prognosis. To date, CA125 is a very sensitive marker of residual epidermal ovarian cancer. In addition, the incre...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/577G01N33/574C07K7/08
Inventor 丁恩雨丁荣新桑恩典
Owner 丁恩雨
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