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Preparation method and application of gastric cancer antigen electrogenerated chemiluminescence sensor based on PP<y>-NH2GO-Ag2Se@CdSe

An electrochemistry and sensor technology, applied in the field of electrochemiluminescence detection, achieves high sensitivity, no radioactive pollution, and simple operation

Inactive Publication Date: 2015-03-04
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a simple, fast and pollution-free PPy-NH 2 GO-Ag 2 Preparation method and application of SeCdSe electrochemiluminescence immunosensor to realize rapid and sensitive detection of CA72-4

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 A PPy-NH based 2 GO-Ag 2 Preparation method of gastric cancer antigen electrochemiluminescence sensor of SeCdSe

[0032] (1) Polish a glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, clean it ultrasonically with ethanol, and rinse it with ultrapure water;

[0033] (2) Dropping 4 μL, 0.5 mg mL -1 PPy-NH 2 GO-Ag 2 The SeCdSe-Ab antibody incubation solution is placed on the surface of the electrode and stored at 4°C until dry;

[0034](3) Add 4 μL bovine serum albumin solution with a mass fraction of 1% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 6.4 PBS buffer solution, and dry at 4°C;

[0035] (4) Add 4 μL of gastric cancer antigen CA72-4 solution dropwise, wash the surface of the electrode with PBS buffer solution of pH 6.4, and place it in a refrigerator at 4 °C for 3 h to dry to prepare a PPy-NH-based 2 GO-Ag 2 SeCd...

Embodiment 2

[0036] Example 2 A PPy-NH based 2 GO-Ag 2 Preparation method of gastric cancer antigen electrochemiluminescence sensor of SeCdSe

[0037] (1) Polish a glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, clean it ultrasonically with ethanol, and rinse it with ultrapure water;

[0038] (2) Dropping 4 μL, 1 mg mL -1 PPy-NH 2 GO-Ag 2 The SeCdSe-Ab antibody incubation solution is placed on the surface of the electrode and stored at 4°C until dry;

[0039] (3) Add 4 μL of bovine serum albumin solution with a mass fraction of 2% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 PBS buffer solution, and dry at 4°C;

[0040] (4) Add 4 μL of gastric cancer antigen CA72-4 solution dropwise, wash the surface of the electrode with PBS buffer solution of pH 7.4, and place it in a refrigerator at 4 °C for 7 h to dry to prepare a PPy-NH-based 2 GO-Ag 2 Se...

Embodiment 3

[0041] Example 3 A PPy-NH based 2 GO-Ag 2 Preparation method of gastric cancer antigen electrochemiluminescence sensor of SeCdSe

[0042] (1) Polish a glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, clean it ultrasonically with ethanol, and rinse it with ultrapure water;

[0043] (2) Drop 4 μL, 2.0 mg mL -1 PPy-NH 2 GO-Ag 2 The SeCdSe-Ab antibody incubation solution is placed on the surface of the electrode and stored at 4°C until dry;

[0044] (3) Add 4 μL of bovine serum albumin solution with a mass fraction of 3% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with PBS buffer solution with pH 8.4, and dry at 4°C;

[0045] (4) Add 4 μL of gastric cancer antigen CA72-4 solution dropwise, rinse the electrode surface with PBS buffer solution with pH 8.4, and incubate in a refrigerator at 4 °C for 12 h to dry to prepare a PPy-NH-based 2 GO-Ag 2 Se...

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Abstract

The invention relates to a preparation method and the application of a gastric cancer antigen electrogenerated chemiluminescence sensor based on PP<y>-NH2GO-Ag2Se@CdSe, belonging to the field of an electrochemical luminescence sensor. An Ag2Se@CdSe nano pin is taken as an electrogenerated chemiluminescence signal source; the Ag2Se@CdSe nano pin is fixedly loaded on the surface of PP<y>-NH2GO by utilizing the large specific surface area of the PP<y>-NH2GO, so that an antibody capture substrate can be prepared; the luminescent signals of the sensor can be effectively enhanced by the excellent electrical conductivity of the PP<y>-NH2GO. The CA72-4 can be detected according to different electrogenerated chemiluminescence signal intensities for the gastric cancer antigen CA72-4 with different concentrations.

Description

technical field [0001] The present invention relates to a kind of based on PPy-NH 2 GO-Ag 2 Preparation method and application of SeCdSe electrochemiluminescence immunosensor. Specifically involving an Ag 2 SeCdSe nanopins as luminescent material, PPy-NH 2 GO-Ag 2 The preparation and application of SeCdSe as an electrochemiluminescence immunosensor for capturing antibody substrates belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Gastric cancer antigen CA724 is a laboratory marker for detecting gastric cancer and various digestive tract cancers. It is called related glycoprotein and belongs to mucin carcinoid embryonic antigen. , lung and ovarian tumors. Therefore, it has certain diagnostic and differential diagnosis significance for tumors in pancreas, ovary, stomach and other parts. [0003] At present, the immunoassay methods for the determination of CA72-4 mainly include radioimmunoassay, enzyme-linked immunoassay...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N21/763G01N33/54386G01N33/57446G01N33/57484
Inventor 吕晓辉魏琴李月云庞雪辉曹伟张勇马洪敏吴丹
Owner UNIV OF JINAN
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