Injection method for single-bird egg

Abstract

The invention relates to a method for injecting to a single bird egg; the operation steps are as follows: the bird egg is positioned at a position with preset direction under incubation temperature; an opening is selected by a position on the egg shell which is to be injected to be disinfected by alcohol; a disinfected solid pin is used to form small openings in the same size on the opening of the egg shell and the outer shell membrane and the inner shell membrane below the opening; the bird egg is slightly rotated, so that the position in the egg to be injected aims to the small openings on the egg shell; a disinfected disposable injection needle is extended into the small openings; materials are injected into the egg by the disposable injection needle; the temperature of the egg is reduced in the environment 1 DEG C to 10 DEG C lower than the incubation temperature; the disposable injection needle is collected; the bird egg rotates for some angles within a longitudinal section, so that the crossing of the injection needle stays from the opening of the egg shell; melted paraffin is used to seal the opening; the paraffin is solidified under the incubation temperature; the bird egg is put to the original place to be incubated. The method has the advantages of low cost, fast operation, single-egg rejection, small simulation and damage, no pollution, fixed point and quantitative injection, and direct view.

Description

technical field [0001] The invention relates to a method for injecting bird eggs, in particular to a method for fixed-point and quantitative injection to a single bird egg, and is especially suitable for injection at each stage of embryos of late-maturing small bird eggs in a natural incubation state. Background technique [0002] Birds are oviparous animals, and their embryonic development is separated from the mother, which is conducive to the use of modern biological technologies such as transgenic technology, embryonic stem cell technology, artificial insemination technology, animal cloning technology and radioisotope labeling and tracing technology to study its individual development . [0003] Compared with larger eggs such as chickens, ducks, geese, etc., the eggs of small birds such as white-rumped munia, parrots, and canaries have the characteristics of thin egg shells, easy opening, and small volume of contents. The success rate of injection is easily affected by ...

AI Technical Summary

Problems solved by technology

This method is not only inaccurate in the injection position, the injection depth is not easy to control, and it will cause great damage to the eggs, and after the injection, the amount of medicine in the needle will be large, which wastes the injection material.

In addition, injection needles are usually recycled, which can easily cause cross-infection and reduce the hatching rate and survival rate of bird eggs

Another shortcoming of the traditional method is that it fails to solve the problem of fixed-point and quantitative injection of small and medium-sized bird eggs, which brings great inconvenience to related research.

The method of Chinese patent 02105130.5 and 99815436.9 is more suitable for industrialized batch injection of large and medium-sized precocious chick eggs (such as chickens, ducks, etc.) of artificial incubation, not only the equipment is expensive, but also the problem of cross-infection in the injection process cannot be eliminated; The problem that the orientation of the bird's eggs during the injection is consistent with the natural hatching orientation of the eggs of the late-growing chicks during the hatching process

(Because both of them need to adjust the orientation of the bird's egg in advance so that the air chamber at the blunt end is upward, and then use the injection needle to inject through the air chamber at the blunt end of the bird's egg) For small bird eggs such as canary eggs and parrot eggs, the volume of the contents in the eggs will increase after injecting a certain volume of substances, and the contents (including the injected substances) will overflow from the opening of the egg shell. It is not easy to complete quantitative injection, and it is not easy to seal. It is also easy to cause environmental pollution for the injection of isotope-labeled substances

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