Biotoxin adsorbent and production method thereof

A biotoxin and adsorbent technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of small contact area of ​​mycotoxins, poor solubility, and unsatisfactory adsorption effect of broad-spectrum mycotoxins, etc. question

Active Publication Date: 2009-02-11
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although glucomannan has a certain biosorption effect on toxins, most glucomanose is extracted from konjac and other plants, which has poor solubility and a small contact area with mycotoxins, and the adsorption effect on broad-spectrum mycotoxins Not ideal yet

Method used

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  • Biotoxin adsorbent and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] Preparation of Yeast Cell Wall Extract

[0032] The yeast cells are then lysed. Lysis can be autolysis or hydrolysis, and any of the methods mentioned in the journal literature can be used to lyse yeast cells. In the present invention, autolysis of yeast cells is performed at a temperature of about 50°C. After lysis, the resulting cell walls were centrifuged several times to remove intracellular material and concentrate the cell walls.

[0033] Cook the obtained yeast cell wall in a cooking pot at a temperature of 80-98°C for 1-2 hours, then lower the temperature to 40-60°C, adjust the pH value to 7-8, and then add an appropriate proportion of biological enzymes, the enzymes used in the process Selected from alkaline protease, mannosidase and amylase or their mixtures, preferably alkaline protease. In a specific embodiment, 1-4‰ of alkaline protease and 1-4‰ of mannosidase are used for enzymatic hydrolysis at 40-60° C. for 15-16 hours. After centrifugation, the cl...

Embodiment 1

[0038] Example 1 Preparation

[0039]300 g of Saccharomyces cerevisiae (Dalian Xinghe Yeast Co., Ltd., China) was autolyzed at a temperature of about 50° C., and the resulting cell walls were centrifuged in a centrifuge at a speed of 6000 rpm to remove intracellular matter and concentrate the cell walls. Cook the obtained yeast cell wall in a cooking pot at a temperature of 80-98°C for 1.5 hours, then lower the temperature to 40-60°C, adjust the pH value to 7-8, and then add 1-4‰ weight (based on the quality of Saccharomyces cerevisiae) of alkali protease and 1-4‰ mannosidase at 43°C for 15-16 hours. After centrifugation, the clear liquid is concentrated, and the obtained clear liquid is spray-dried to obtain hygroscopic water-soluble powder mannan oligosaccharides. Spray-dry to obtain dry powder with mannan oligosaccharide content reaching 40%-50%. The dry powder was uniformly mixed with aluminosilicate at a weight ratio of 98:2 to obtain a biotoxin adsorbent (product 1). ...

Embodiment 2

[0040] Example 2 Preparation

[0041] Autolyze 300 g of Saccharomyces cerevisiae (obtained by amplified culture of the Saccharomyces cerevisiae strain M205128 cultivated by the applicant company) at a temperature of about 50°C, and centrifuge the obtained cell wall in a centrifuge at a speed of 6000 rpm to remove intracellular matter and condense the cell wall. Cook the obtained yeast cell wall in a cooking pot at a temperature of 80-98°C for 2 hours, then lower the temperature to 40-60°C, adjust the pH value to 7-8, and then add 1-4‰ weight (based on the quality of Saccharomyces cerevisiae) of alkali protease and 1-4‰ mannosidase at 60°C for 15-16 hours. After centrifugation, the clear liquid is concentrated, and the obtained clear liquid is spray-dried to obtain hygroscopic water-soluble powder mannan oligosaccharides. Spray-drying yields a dry powder with a manno-oligosaccharide content of 48%. The dry powder and the aluminosilicate are uniformly mixed in the weight rati...

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Abstract

The invention provides a product for absorbing toxin in feed, which comprises 1 to 10 percent of clay and 90 to 99 percent of leaven nectar oligose by weight. The invention provides a manufacturing method and a compound product to absorb toxin in feed, the nectar oligose is extractive of leaven cytoderm and aluminosilicate. The leaven cytoderm is extracted from saccharomyces cerevisiae. The biotoxin adsorbent can be fed to any animals including fowl, livestock, marine lives, ruminant and the like. The substance reduces the amount of toxin entering animal body through stronger adsorption capacity when feeding with feed or as additive, thus improving animal productivity and health condition and reducing the diseases caused by toxin.

Description

technical field [0001] The invention relates to a biotoxin adsorbent and a production method thereof. Background technique [0002] Every year, a significant portion of the grain raw materials used for animal feed is contaminated with toxins produced by molds. The reduction in the nutritional value of the feed and the incidents of animal poisoning are due to the growth of molds such as Aspergillus niger, aflatoxin and Penicillium in grain or other feed ingredients during storage. Mycotoxins can affect the nutritional value of feed, animal performance and animal health. [0003] The physical effects of mycotoxins are many, ranging from reduced feed intake, lower feed conversion ratios, to an overall reduction in animal growth. Mycotoxins can also be a hazard to human health, as many toxins get into milk or meat as animals ingest them. [0004] Acute symptoms caused by mycotoxins are relatively easy to identify. However, chronic symptoms such as slightly reduced productivi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/17C12P19/14C12R1/865C12N1/16A23K20/195
CPCB01J20/12A23K1/188A23K1/1643B01J2220/46B01J20/24A23K1/182B01J20/16B01J20/28004A23K1/1813A23K1/1756A23K20/163A23K20/28A23K50/10A23K50/70A23K50/80
Inventor 俞学锋李知洪余明华姚娟谭斌朱金林
Owner ANGELYEAST CO LTD
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