Bulk medicament for treating orthopedic disorders and preparation method thereof

A technology for orthopedic diseases and raw materials, applied in bone diseases, medical formulas, plant raw materials, etc., can solve the problem of the preparation method of high-purity Dipsacus saponin VI reported, and the application of high-purity Dipsacosa saponin VI in orthopedic diseases Reporting and other issues

Active Publication Date: 2009-02-18
博济医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, more than 20 kinds of saponins have been separated from Dipsacus medicinal materials, but there is no report on the preparation method of high-pu

Method used

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  • Bulk medicament for treating orthopedic disorders and preparation method thereof
  • Bulk medicament for treating orthopedic disorders and preparation method thereof
  • Bulk medicament for treating orthopedic disorders and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Take 1 kg of Dipsacus, extract twice with 8L 70% ethanol, reflux for 2 hours each time, combine the extracts, concentrate under reduced pressure, recover the ethanol until there is no alcohol smell, add water to dilute to a liquid containing 1.0g of medicinal materials per ml, and use 5% Adjust the pH of the calcium hydroxide suspension to 8-9, let it stand for 12 hours, take the supernatant, and filter to obtain the filtrate. Add the filtrate to the chromatographic column of 1.0kg SA-2 macroporous resin, and after cyclic adsorption twice, use 3L of 1.0% KOH to elute, then wash with water until nearly neutral, then use 3L of 30% ethanol to elute, before discarding Two eluents; finally washed with 7L of 60% ethanol, collected eluate, decolorized with 0.5% activated carbon, filtered, recovered ethanol under reduced pressure, concentrated and dried to obtain 80.9 grams of light yellow Dipsacus saponin VI crude product.

[0047] Add water to the crude product of Dipsacus sa...

Embodiment 2

[0049] Take 1 kg of Dipsacus, crush it, soak it in 6L of water-saturated n-butanol overnight, then start percolating, collect n-butanol, extract it with an equal volume of n-butanol saturated water for 3 times, recover n-butanol under reduced pressure until it is nearly dry, add water Dilute to a liquid containing 0.8g medicinal material per milliliter, let it stand for 12 hours, filter, take the filtrate, add the filtrate to the chromatographic column of 1.5kg ADS-5 macroporous resin, circulate and adsorb for 3 times, wash with 3L of 0.5% NaOH Then wash with water until it is nearly neutral, then use 3L of 30% ethanol to elute, discard the first two eluents, and finally wash with 5L of 70% ethanol, collect the 70% ethanol eluate, and decolorize it with D208 decolorizing resin. The ethanol was recovered under reduced pressure, concentrated and dried to obtain 52.6 g of crude product of light yellow Dipsacus saponin VI.

[0050] Soak 200g of reverse-phase column ODS packing mat...

Embodiment 3

[0052] Take 1 kg of Dipsacus, crush it, moisten it with 1 times the amount of 80% ethanol, soak it in 6L of 80% ethanol for 15 hours, and then start percolation, 5ml / min, collect the percolation liquid, concentrate under reduced pressure, and recover the ethanol until it has no alcohol smell Finally, add water to 3000ml, let it stand for 4 hours, filter, take the filtrate, add the filtrate to the chromatographic column of 1.2kg ADS-8 macroporous resin, after circulating and adsorbing for 3 times, elute with 4L of water, and then use 4L of 30 % ethanol for elution, discard the eluent, discard the first two eluents, then rinse with 4L 90% ethanol, collect the 90% ethanol eluate, and decolorize the 90% ethanol eluate per acidic alumina column (6.0 cm × 8.0cm), ethanol was recovered under reduced pressure, concentrated, and vacuum-dried to obtain 51.3 grams of crude product of Dipsacus saponin VI in light yellow color.

[0053] Take 8 g of the crude product of Dipsacus saponin VI ...

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Abstract

The invention discloses a bulk drug for treating orthopaedic diseases. The bulk drug is Chinese teasel root saponin VI and is prepared by extracting traditional Chinese medicinal materials which contain the Chinese teasel root saponin VI; the weight percentage content of the Chinese teasel root saponin VI occupies 95.0 to 99.9 percent of the total amount of an extract of the Chinese teasel root saponin VI; and the chemical name of the bulk drug is 3-O-alpha-L-arabopyranose ivy sapogenin-28-O-beta-D-glucopyranose(1-6)-beta-D-glucopyranoside. The invention also discloses a method for preparing the bulk drug - the Chinese teasel root saponin VI. The method comprises the following steps of extraction, concentration, separation of the total saponin, decolorization and purification and refinement. The preparation method has the advantages that the preparation method has high extraction rate and low cost, is safe and environment-friendly and is suitable for industrialized mass production. The invention also provides application of the bulk drug in preparing medicines for preventing and treating the orthopaedic diseases including osteoporosis, fracture and osteoarthritis.

Description

technical field [0001] The invention relates to a raw material drug for treating orthopedic diseases, and also relates to a preparation method of the raw material drug for treating orthopedic diseases. Background technique [0002] Dipsacus is the dry root of Dipsacus asperoides.Y.Cheng et T.M.Ai, a plant of Dipsacaceae. It was first recorded in "Shen Nong's Materia Medica", and it was listed as the top grade. It is recorded that it is "bitter in taste, slightly warm, mainly for typhoid fever, tonic for insufficiency, gold sores, carbuncles, folds, joints and bones, and difficult for women to breastfeed. Long-term use can benefit energy. A The name is Longdou, and the other is Zhe.” Li Shizhen said, “Dusan, Zhe, and Bone are all named after merit.” From its name, it can be seen that Dipsacan has been an important medicine for orthopedics since ancient times. According to the current Pharmacopoeia: "It tastes bitter, pungent, and slightly warm. It returns to the liver and ki...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K36/185A61P19/00A61P19/10A61P19/02
Inventor 马仁强翟涛
Owner 博济医药科技股份有限公司
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