Method of cottage and breeding for caxillary bud of pineapple in-vitro

A technology of cutting propagation and axillary buds, which is applied in the agricultural field, can solve the problems of high cost of seedling cultivation, low reproduction rate and high technical requirements, and achieve the effect of mass production, high reproduction rate and easy technology

Inactive Publication Date: 2009-02-25
何光浚
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to design the method for isolated cuttage propagation of pineapple axillary buds, to overcome the method of branching propagation using crown buds, support buds, suction buds and other organs of pineapple, because a pineapple plant only has crown buds, support buds, suction buds, etc. There are 5 to 6 buds in total, the reproduction rate is not high, and the use of tissue culture technology to raise seedlings has the disadvantages of high technical requirements, large investment, high cost of seedling cultivation, and difficulty in popularization

Method used

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Embodiment Construction

[0007] The method of in vitro cutting propagation of pineapple axillary buds is: first peel off the axillary buds of the pineapple, and then cut the axillary buds in the seedbed. The cutting depth is between 5 and 40 mm. After the axillary buds are cut, keep the soil moist every day. ——35 degrees Celsius until the pineapple seedlings grow.

[0008] Can follow the steps when concretely implementing the present invention:

[0009] 1. Sterilization of crown buds: Cut the crown buds from the pineapple fruit and soak them in 20% lime water for 5 minutes for sterilization.

[0010] 2. Stripping of axillary buds: Use a sharp scalpel or a small knife to cut off the axillary buds in vitro along the periphery of the pineapple crown bud stem, select healthy and uninjured axillary buds, and eliminate damaged axillary buds.

[0011] 3. Root-promoting treatment of axillary buds: Put the selected strong and undamaged axillary buds with leaves into 1% rooting solution No. 1 for strong rootin...

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Abstract

The invention relates to a pineapple axillary bud in vitro cutting propagation method which is characterized in that the pineapple axillary buds are sheared, and then are rooted on a seedling bed; the soil is kept moist every day after the axillary buds are rooted, the seedling environment temperature is controlled at 18 to 35 DEG C till the pineapple seedlings are grown out. The propagation method has little investment, the technique is easy to master, 20 to 30 axillary buds can be obtained from one pineapple crown bud, each axillary bud can be cultivated into a pineapple plant, so the propagation rate is high, and the propagation method can meet the requirements of mass production.

Description

1. Technical field [0001] The invention belongs to the technical field of agriculture, in particular to the improvement of a method for in vitro cutting propagation of pineapple axillary buds. 2. Background technology [0002] Pineapple (Ananas comosus), also known as pineapple, is a perennial evergreen herbaceous fruit plant of the genus Bromeliaceae. Pineapple grows rapidly and has a short production cycle, which is a fruit of great economic value. Two methods are currently used for the propagation of pineapple: one is to use the pineapple's crown buds, support buds, and suction buds to carry out branch propagation. With 5 to 6 buds, the reproduction rate is not high, which cannot meet the needs of mass production. The second method is to adopt tissue culture technology to grow seedlings. Although this method can meet the requirements of mass production, it has high technical requirements, large investment, high cost of seedling cultivation, and great difficulty in popul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
Inventor 何光俊
Owner 何光浚
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