Northeast wood frog and molecular identification method for egg oil thereof

Abstract

A molecular identification method for the wood frog in the northeast of China and the ovum oil thereof aims at solving the identification of the wood frog in the northeast of China and the product ovum oil thereof, which is mainly based on the property characteristics, microscopic characteristics, physical and chemical properties, and the like at present; as the bases are lack of clear shape identification characteristics and special chemical compositions, the identification for the ovum oil in practice is still very difficult and the invention is hereby designed. The identification method takes PCR technology as the basis and screens specific primers for amplification so as to achieve the molecular identification of the wood frog in the northeast of China and the ovum oil thereof, comprising the following steps: (1) extraction of genomic DNA; (2) genomic DNA detection and concentration determination; (3) PCR amplification; and (4) PCR amplification product identification. The invention is characterized in that the invention starts from the basic theory of molecular biology, uses modern molecule marker technology for conducting molecular identification of the wood frog in the northeast of China and the ovum oil thereof, has the advantages of scientific and credible theoretical basis, advanced and reasonable technical means and achieving steps, reliable detection result, strong specificity of screened primers as well as high stability of agarose gel electrophoresis characteristic bands, and can obtain reliable and useful effects in practical application.

Description

Technical field: [0001] The invention relates to a biological molecular identification method, which is mainly aimed at the molecular identification method of the Northeast Rana species and its product egg oil. Background technique: [0002] Northeast Rana has important economic significance and medical care value. The dried product of female frog oviduct is used as medicine, which is called wood frog oil or husky frog oil, which is a precious animal medicinal material. The identification of this medicinal material is still mainly based on its traits, microscopic characteristics, and physical and chemical properties. However, due to the lack of obvious morphological identification characteristics and special chemical components of this medicinal material, it is still very difficult to identify Rana ovale in practice. Invention content: [0003] Aiming at the problem that it is difficult to identify the Northeast Rana and Rana egg oil in terms of traits, microscopic charac...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that it is difficult to identify the Northeast Rana and Rana egg oil in terms of traits, microscopic characteristics, physical and chemical properties, etc. in the production and management practice, the present invention uses molecular biology technology to solve the problem of Rana Rana egg oil and its medicinal source animal Northeast China. The molecular identification of wood frog provides a method based on PCR technology to screen specific primers for amplification, so as to achieve molecular identification of Northeast wood frog and its egg oil. The specific implementation steps are:

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