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Flow-type cell instrument and waste liquid discharge device and method thereof

A flow cytometer and waste liquid discharge technology, which is applied in the direction of instruments, material inspection products, and analytical materials, can solve the problems affecting the stability of the sheath flow in the flow chamber and achieve good results

Active Publication Date: 2013-08-28
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a new flow cytometer and a method for eliminating the fluid disturbance of the flow cytometer, so as to overcome the problem that the waste liquid pump affects the stability of the sheath flow in the flow chamber in the above-mentioned prior art

Method used

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  • Flow-type cell instrument and waste liquid discharge device and method thereof
  • Flow-type cell instrument and waste liquid discharge device and method thereof

Examples

Experimental program
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specific Embodiment approach 1

[0028] The flow cytometer provided in this specific embodiment is as image 3 As shown, it includes a sample pool 1 , a negative pressure pool 2 , a sample syringe 3 , a sheath fluid pool 4 , a waste fluid pool 5 , a flow chamber 6 and a waste fluid pump 9 . The flow chamber 6 has a sheath fluid inlet 8 for the sheath fluid to flow in, a sample needle 7 for injecting the sample to be tested, and an outlet for the waste fluid to discharge. The sample pool 1 is used for storing well-reacted samples. The negative pressure pool 2 is connected with an external negative pressure source, so that the negative pressure is permanently maintained in it, and is used for sample preparation. Sample preparation is to draw the sample in the sample pool 1 into the pipeline between the sample syringe 3 and the flow chamber 6, and the sample in the pipeline is the sample to be tested. The sample injector 3 is used to drive the sample to be tested into the flow chamber 6 through the sample need...

specific Embodiment approach 2

[0030] The flow cytometer provided in this specific embodiment, such as Figure 4As shown, it includes a sample pool 1, a sample syringe 3, a sheath liquid pool 4, a waste liquid pool 5, a flow chamber 6, a waste liquid pump 9 and a constant flow drive device. The flow chamber 6 has a sheath fluid inlet 8 for the sheath fluid to flow in, a sample needle 7 for injecting the sample to be tested, and an outlet for the waste fluid to discharge. The sample pool 1 is used for storing well-reacted samples. The sheath fluid pool 4 is used to store the sheath fluid. The waste liquid pool 5 is used for storing waste liquid. The waste liquid pump 9 is used to provide driving force for the discharge of the waste liquid in the sample pool 1 . In this specific embodiment, the constant flow driving device is a sheath flow injector 21, which is used for sample preparation and suction of the sheath fluid in the sheath fluid pool 4, and provides a drive to inject the sheath fluid into the fl...

specific Embodiment approach 3

[0035] The flow cytometer of this specific embodiment, such as Figure 5 As shown, the difference from Embodiment 2 is that the tee joint 12 is omitted, and the volume container 10 itself has two inlets and one outlet. The waste liquid discharged from the flow chamber 6 through the first branch pipe enters the volume container 10 through one inlet; Discharge to the waste liquid pool 5 through the waste liquid discharge pipe. Simultaneously, in order to make the volume of the volume container 10 a little smaller, to reduce its volume, in the pipeline between the volume container 10 and the flow chamber 6, a flow-restricting tube 11 is connected in series, so when the volume of the volume container 10 is small It can also better isolate pressure fluctuations.

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Abstract

The invention discloses a flow cytometry and a waste liquid eductor thereof and a method. The waste liquid eductor of the flow cytometry comprises a waste liquid discharge pipe, a first branch pipe, a second branch pipe and a pressure fluctuation isolated unit; the first branch pipe is connected with an inlet end of the waste liquid discharge pipe and an outlet of a streamer chamber; the second branch pipe is connected with a waste liquid pump of the flow cytometry and the inlet end of the waste liquid discharge pipe; the pressure fluctuation isolated unit is connected in the inlet end of the waste liquid discharge pipe and the pipeline of the streamer chamber in series. Use of the flow cytometry in the inventive technical scheme, due to the pressure fluctuation isolated unit, effectively isolates unstable fluid caused by pressure fluctuation generated by the waste liquid pump, therefore, no adverse effect is caused on the sample detection in the streamer chamber when the waste liquid pump works.

Description

technical field [0001] The invention relates to the technical field of cell detection and analysis, in particular to a waste liquid discharge device for a flow cytometer, a flow cytometer including the waste liquid discharge device, and a waste liquid discharge method for a flow cytometer. Background technique [0002] In the field of blood cell detection and particle analysis, the cells or particles to be detected are often queued to pass through a small hole, and detected by optical or electrical methods at the small hole, and the number, volume information and internal characteristics of cells or particles are analyzed. In order to make the path of cells or particles through the small hole more regular, a clean liquid (sheath fluid) can be introduced to wrap around the sample to be tested, so as to ensure that the cells or particles are in the middle of the small hole when they line up through the small hole, thereby Easy to detect. The principle is as figure 1 As shown...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48G01N15/00
Inventor 甘泉许华明赵丙强
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD