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Method for preparing chitosan/protein composite micrographics on surface of material

A technology of protein compounding and micro-patterning, which is applied in printing plate copying, medical science, printing, etc., can solve the problems of increasing the potential toxicity of materials, small template load capacity, and few biomolecules, and achieve good biocompatibility and product quality. Shorter cycle times, less potential toxic effects

Inactive Publication Date: 2012-11-21
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that in the process of pretreatment of the substrate, additional aldehyde functional groups are introduced, which increases the potential toxicity of the material, and the micro-junction printing method only prints the biomolecule solution to be assembled on the surface of the template. The carrying capacity of the template is small, the prepared biomolecules are relatively small, and the biological effect is correspondingly weak

Method used

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  • Method for preparing chitosan/protein composite micrographics on surface of material
  • Method for preparing chitosan/protein composite micrographics on surface of material
  • Method for preparing chitosan/protein composite micrographics on surface of material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Take a pure silicon wafer as the base material, and sinter it at 1000°C for 4 hours to obtain a dense and uniform oxide layer on the silicon surface, and ultrasonically pass through ethanol and water;

[0030] (2) Dissolve 40g of NaOH in 500ml of distilled water to prepare a 5mol / L alkali solution. Treat the silicon wafer prepared in (1) with alkali in a 60°C water bath for 3 hours, clean it with distilled water, and dry it to obtain a sufficient surface. Hydroxy;

[0031] (3) Dissolve 0.4 g of acetic acid in 19.6 g of distilled water to prepare 20 ml of acetic acid solution with a mass fraction of 2%, then weigh 40 mg of CS and add it to the acetic acid solution, and stir evenly to obtain a 2 mg / ml CS solution;

[0032] (4) Take 0.087g NaH 2 PO 4 , 0.135g Na 2 HPO 4 and 0.085g NaCl were dissolved in 20ml of distilled water, and the pH value was adjusted to 6.6 with HCl or Tris to prepare a phosphate buffer solution; 40mg of BSA was dissolved in the phosphate bu...

Embodiment 2

[0059] The bovine serum albumin of embodiment 1 is changed into collagen protein, gets collagen protein and is dissolved in phosphate buffer saline, is mixed with 2mg / ml collagen protein solution, other conditions are constant, utilizes experimental method of the present invention, prepares chitosan / Composite micropatterns of collagen.

Embodiment 3

[0061] Replace the pure silicon wafer in Example 1 with a pure titanium wafer, put the pure titanium wafer into a 5 mol / L NaOH solution after ultrasonication with acetone and water, treat it with alkali at 60°C for 3 hours, clean it with distilled water, and dry it , that is to obtain sufficient and uniform hydroxyl groups on the titanium surface, and other conditions remain unchanged, using the experimental method of the present invention to prepare a composite micropattern of chitosan / bovine serum albumin on the surface of the titanium substrate.

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Abstract

The invention discloses a method for preparing a chitosan / protein composite micrographic on the surface of a material. The method comprises the following steps that: silicon is selected as a substrate; the silicon is oxidized by dry-oxygen at 1,000 DEG C, so that the surface of the silicon obtains a compact and even oxide film; and the silicon is treated by alkali, and is washed and dried, so that a silicon chip full of hydroxide radical on the surface is obtained; bovine serum albumin is prepared into a solution with certain concentration with phosphate buffer, chitosan and acetic acid respectively; a slot shaped micrographic of the chitosan is prepared on the hydroxylated silicon surface by a microtransfer molding method; a slot shaped micrographic of the protein is crossly prepared on the coating; and finally, a chitosan / protein composite micrographic with regular shape on the surface of the material is obtained.

Description

Technical field [0001] The invention relates to a method for preparing chitosan / protein composite micropatterns on the surface of materials. The composite micropattern has antibacterial properties and promotes the positioning, adsorption and growth of osteoblasts. Background technique [0002] Many materials can guide the growth of cells, but some specific tissues such as nerves, bones, blood vessels and cornea require cells to be guided by more specific signals when they are regenerated in vivo. Therefore, an ideal material should be able to selectively participate in the specific adhesion and growth factor receptor interaction expressed by the target cells in the repair tissue. Studies have shown that a variety of cells can respond to the surface topology of materials at the micrometer scale or even at the nanometer scale. The morphology, orientation, growth and differentiation of cells are also affected by the surface structure. In order to study the process of interacti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04B41C3/04
Inventor 鲁雄谢佳周先礼姜丽丽张红平屈树新冯波翁杰汪建新
Owner SOUTHWEST JIAOTONG UNIV