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Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use

A technology of nitrotoluene and Rhodococcus is applied in the field of microorganisms and achieves the effects of convenient operation and simple preparation method

Inactive Publication Date: 2009-07-08
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report that purely cultured microorganisms can completely degrade 3-nitrotoluene compounds at home and abroad.

Method used

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  • Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use
  • Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use

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Embodiment Construction

[0024] The invention provides a preparation method and application of Rhodococcus ZWL3NT strain, which will be further described below in conjunction with the examples and accompanying drawings.

[0025] One. The preparation method of Rhodococcus bacterial strain ZWL3NT

[0026] 1. Preparation of inorganic salt medium:

[0027] Composition is Na 2 HPO 4 12H 2 O 14.3g, KH 2 PO 4 3.0g, MnSO 4 ·H 2 O 0.28mg, FeSO 4 ·7H 2 O0.3mg, MgSO4 0.06mg, CaCl 2 1mg, CuSO 4 0.05mg, ZnSO 4 0.05mg and H 3 BO 3 0.05mg, dilute it to 1000ml with double-distilled water, adjust the pH to 7.0, and sterilize at 121°C and 15 pounds for 30 minutes under high pressure.

[0028] 2. Enrichment of degrading bacteria:

[0029] Take soil samples from Shandong Huayang Pesticide Co., Ltd., add 5-20 grams of soil to 100 ml of inorganic salt medium to prepare a suspension, and then take 5-10 ml of soil suspension to inoculate 100 ml of 1mM 3-nitrotoluene In the inorganic salt medium, the temp...

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Abstract

The invention discloses a Rhodococcus strain ZWL3NT CCTCC NO: M 208206. The preparation method of the strain includes formulation of an inorganic salt substrate, strain concentration, separation and degradation of bacteria, wherein, the inorganic salt substrate contains Na2HPO4*12H2O, KH2PO4, MnSO4*H2O, FeSO4*7H2O and other components; the bacterial concentration includes: formulating the soil sample and inorganic salt substrate into a suspending liquid, inoculating the suspending liquid in a certain volume of inoculating amount onto a fresh inorganic salt medium containing 3-nitrotoluene, shaking table culturing at constant temperature until obtaining the accumulation culture object with significant degradation performance; the separation adopts a plating technique to separate and purify the culture in the LB medium. The bacterial strain provided by the invention can be used to degrade the 3-nitrotoluene with the degradation rate of 99 percent, and the preparation method is simple and easy to operate.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a rhodococcus strain for degrading 3-nitrotoluene, a preparation method and application thereof. The Rhodococcus strain ZWL3NT (Rhodococcus sp. ZWL3NT) was preserved by the China Center for Type Culture Collection, the preservation date is November 10, 2008, and the preservation number is CCTCC NO: M208206. Background technique [0002] Nitrotoluene compounds include three isomers, namely: 3-nitrotoluene (3-nitrotoluene, 3NT), 2-nitrotoluene (2-nitrotoluene, 2NT), 4-nitrotoluene (4-nitrotoluene, 4NT). As raw materials and intermediates, it is widely used in the production and manufacture of various dyes, pesticides, and pharmaceuticals. They have many ways to enter the environment as pollutants during the production process. The harm of nitrotoluene compounds to the human body is similar to that of nitrobenzene. It can be rapidly absorbed through inhalation, ingestion and skin. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/00
Inventor 王淑君刘虹周宁一
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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