X chromosome MiniSTR fluorescent composite amplification reagent kit, preparation and use thereof

A technology of X chromosome and compound amplification, which is applied in the fields of fluorescent compound amplification kits and PCR amplification kits, and can solve the problems of low degree of automation, limitation of the number of loci and personal identification ability, and inability to meet higher requirements, etc.
CN101475993AInactive Publication Date: 2009-07-08CHINA UNIVERSITY OF POLITICAL SCIENCE AND LAW
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA UNIVERSITY OF POLITICAL SCIENCE AND LAW
Publication Date
2009-07-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an X chromosome parting kit and its preparation method and application. The present invention designs four pairs of public primer pairs, optimizedly select 12 X chromosome STR loci suitable for the genetic distribution of Chinese population and designs MiniSTR primers fro part of loci, and respectively adds the four pairs of public primers to the 5' end of the MiniSTR primers to obtain 24 additional primers; the use of the inventive kit can simultaneously amplify 12 X chromosome STR loci at a single-tube, combined with genetic markers of autosomal, Y chromosome and mitochondrial, the invention can effectively solve sister identification lack of parents, half-sister identification, atavism identification and the like special pro-prosecution case, at the same time can realize the successful detection of DNA template as low as 0.05ng / 20mul. The inventive kit has advantages of high sensitivity, high detection rate of samples of a high degree of DNA degradation, low preparation cost and the like.
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Description

technical field

[0001] The invention relates to a PCR amplification kit, in particular to a fluorescent multiplex amplification kit for simultaneously detecting 12 X chromosome MiniSTR loci in a single tube. The present invention also relates to a preparation method of the fluorescent multiplex amplification kit and the The application of the kit in the field of judicial identification belongs to the field of X chromosome typing and identification. Background technique

[0002] Short tandem repeat (short tandem repeat, STR) is a type of DNA sequence with length polymorphism formed by tandem repetition of 2 to 6 bases as the core unit in the human genome. The number of core units varies and the number of repetitions is different. The genetic polymorphisms that make up the STR. STR is widely distributed and numerous, accounting for about 10% of the human genome. The genetic information contained in it is unmatched by any polymorphic markers in the past. Therefore, STR is curr...

Claims

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