Metal nano material toxicity detection board and detection method thereof

A toxicity detection, metal nanotechnology, applied in the direction of material excitation analysis, biochemical equipment and methods, color/spectral characteristic measurement, etc., can solve the problems that the detection results are not necessarily reliable, easy to introduce errors, and inconvenient to compare, etc., to achieve detection Accurate and reliable results, elimination of operating errors, easy and repeatable effects

Inactive Publication Date: 2009-07-15
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, because there are many factors affecting the thiazole blue colorimetric method, it is easy to introduce errors, and there is no international unified technical standard support for this method. Different institutions and co...

Method used

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  • Metal nano material toxicity detection board and detection method thereof
  • Metal nano material toxicity detection board and detection method thereof
  • Metal nano material toxicity detection board and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Such as figure 1 As shown, the metal nanomaterial toxicity detection plate in this embodiment includes: a base 1, a plate cover 2 and 24 detection pools 3, wherein: the base 1 is a rectangular container, and 35 vertical positioning columns are arranged on the inner bottom surface of the base 1 4 so that the edge of the detection pool 3 is positioned on the base 1, and the plate cover 2 is provided with buckle edges 5 to fasten the edge of the base 1.

[0073] Both the base 1 and the cover 2 are made of transparent polystyrene.

[0074] The frame around the lower surface of the plate cover 2 is provided with micro-through holes 6 for the purpose of permeating carbon dioxide gas when the cells are cultured.

[0075] The positioning columns 4 are arranged on the inner bottom surface of the substrate 1 in a matrix of 4*6, and each detection pool 3 is provided with four positioning columns 4 in the vertical and horizontal directions, and the center distance between adjacent...

Embodiment 2

[0096] This embodiment relates to the detection method of the metal nanomaterial toxicity detection plate comprising the following steps, wherein the metal nanomaterial is magnetic Fe modified by pullulan 3 o 4 nanoparticles. In embodiment 2, the metal nanomaterial toxicity detection plate identical to embodiment 1 is adopted to carry out the detection of the following steps:

[0097] ① Preparation of metal nanomaterials to be tested and cell samples to be tested:

[0098] Following the same steps as in Example 1, pullulan and bare magnetic nanoparticles were added to the cell culture medium at a mass ratio of 0.2:100 to prepare the metal nanomaterial to be detected. Select the normal human liver cell line HL-7702 at 1.5 x 10 per well 4 The density was inoculated in the 24-well assay plate in Example 1, the volume was 400 μL / well, and the nanoparticle mixture sample was added to make the final concentrations were 12.5, 25, 50, 100, 250 μg / mL, and the final volume was 400 μL...

Embodiment 3

[0111] This embodiment relates to the detection method of the metal nanomaterial toxicity detection plate comprising the following steps, wherein the metal nanomaterial is dextran-modified magnetic Fe 3 o 4 nanoparticles. In embodiment 3, the metal nanomaterial toxicity detection plate identical to that of embodiment 1 is adopted to carry out the detection of the following steps:

[0112] ① Preparation of metal nanomaterials to be tested and cell samples to be tested:

[0113] Following the same steps as in Example 1, dextran and bare magnetic nanoparticles were added to the cell culture medium at a mass ratio of 1.62:100 to prepare the metal nanomaterial to be detected. Select human lung normal cell line HBE to 2 x 10 per well 4 The density was inoculated in the 24-well assay plate in Example 1, specifically 400 μL / well, and the nanoparticle mixture sample was added to make the final concentrations respectively 12.5, 25, 50, 100, and 250 μg / mL, and the final volume was 400...

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Abstract

The invention relates to a metallic nano material toxicity detection board and a detection method thereof in the nano technical field. The toxicity detection board comprises a base, a board cover and a detection cell, and is characterized in that the base is a rectangular container; a plurality of vertical positioning columns are arranged on the inside bottom of the base so that the edges of the detection cell can be located on the base; and clasping edges are arranged at the periphery of the board cover to clasp the edges of the base. The method of the invention takes the possible degradation and metabolic components inside modification molecules into full consideration, and determines the cytotoxicity grade of metallic nano particles through a double step detection method, so that the selection of proper metallic nano particles and the modification molecules in future can be standardized and unified, and easy to repeat and compare.

Description

technical field [0001] The invention relates to a detection method in the field of nanotechnology, in particular to a metal nanomaterial toxicity detection plate and a detection method thereof. Background technique [0002] Nanoparticles made of simple metal elements or metal compounds have broad application prospects in the field of biomedicine, such as gold nanoparticles, silver nanoparticles, magnetic nanoparticles, titanium dioxide nanoparticles, and so on. These nanomaterials have application prospects in medical diagnosis, disease treatment, and target recognition. However, as nano-scale particles, they may have adverse effects on human health, living environment and even all aspects of the life process. Therefore, in clinical applications related to diagnosis and treatment, it is necessary to select the appropriate type of metal nanoparticle material; At the same time, it is necessary to modify the surface of nanoparticles with a layer of molecules with better biocom...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/18G01N21/31G01N21/64
Inventor 龙玲尹龙萍刘睿黄碧兰何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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