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Application of oleanolic acid in preparing sea environment-friendly antifouling agent

An oleanolic acid and marine technology, which is applied in the field of antifouling agents, can solve problems such as no marine antifouling agent with oleanolic acid, and achieve the effects of good antifouling effect, good lipophilicity and wide application prospect.

Inactive Publication Date: 2012-05-30
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there have been no reports about oleanolic acid being used as a marine antifouling agent

Method used

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  • Application of oleanolic acid in preparing sea environment-friendly antifouling agent
  • Application of oleanolic acid in preparing sea environment-friendly antifouling agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Detection of Oleanolic Acid's Inhibitory Effect on Marine Micro-fouling Organisms

[0014] The bioassay model for marine micro-fouling organisms uses marine fouling bacteria, the most dominant micro-fouling organisms in the ocean. Pseudomonas sp., a marine fouling bacterium, was isolated and purified from the surface of steel plates in seawater in Xiamen sea area. Bacteria were inoculated in liquid medium, cultured overnight on a shaker, and collected by centrifugation, washed with sterile seawater and made into a suspension with a bacterial concentration of about 10 7 ~10 9 cell / ml. Oleanolic acid was first dissolved in a small amount of ethyl acetate, then mixed with the prepared bacterial suspension at a concentration of 0.1, 1, 10 and 100 μg / ml, and added to a petri dish. In addition, the petri dish added only with bacterial suspension and a small amount of ethyl acetate without oleanolic acid was used as the control group. Three parallel cups were set...

Embodiment 2

[0017] Example 2 Detection of the inhibitory effect of oleanolic acid on marine macro-fouling organisms

[0018] The biological detection model of marine macrofouling organisms adopts the most important macrofouling organisms in the ocean-barnacles. The adults of Balanus albicostatus were collected from reefs and bridge piers on the coast of Baicheng, Xiamen City, and their Venus larvae were cultured in the laboratory. It is the attachment of the larvae that causes the barnacles to switch from a planktonic to an attached life, so inhibition of the attachment of the larvae is evidence of the antifouling activity of the compound. Dissolve oleanolic acid in ethyl acetate, set the concentration gradient to 0.05, 0.25, 0.5, 2.5, 5 and 25 μg / ml, take 10ml of each solution and add it to the petri dish, and add another 10ml of ethyl acetate to the blank In the petri dish, set it as the corresponding control group. After the solvent is completely evaporated and the extracts are evenly...

Embodiment 3

[0021] Example 3 Antifouling Effect Evaluation of Oleanolic Acid in Natural Sea Areas

[0022] The antifouling efficacy of the oleanolic acid antifouling agent of the present invention in the sea area of ​​Xiamen was tested with reference to the national standard "Shoal Sea Immersion Test Method for Antifouling Paint Samples" (GB / T 5370-2007). After 38 days of hanging in the natural sea area, the biofouling on the surface of the three oleanolic acid test samples was significantly lower than that of the blank sample, indicating that oleanolic acid has a good antifouling effect.

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PUM

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Abstract

The invention discloses application of oleanolic acid in preparing a marine environment-friendly antifouling agent, and relates to an antifouling agent, in particular to application of a natural active material oleanolic acid as the marine environment-friendly antifouling agent. The oleanolic acid applied to a marine antifouling coating can prevent marine plants from adhering and growing on the surfaces of submarine artificial facilities such as the bottom of a ship, an offshore oil-gas platform, a buoyage, a wharf, a bridge pier, a seawater pipeline, an aquaculture net cage, and the like. The invention provides application of the oleanolic acid in preparing the marine environment-friendly antifouling agent and the marine antifouling coating.

Description

technical field [0001] The present invention relates to an antifouling agent, in particular to the use of oleanolic acid, a natural active substance extracted from plants, as an environmentally friendly marine antifouling agent. It is used in marine antifouling coatings and can prevent marine organisms from Adhesion and growth on the surface of artificial facilities in the sea such as ship bottoms, offshore oil and gas platforms, buoys, wharves, bridge piers, seawater pipelines, and cage nets. Background technique [0002] In the marine environment, marine organisms such as marine benthic microorganisms, macroalgae, sponges, coelenterates, bryozoans, scutellaria, bivalves, barnacles, and sea squirts must find suitable substrates for attachment. The surfaces of man-made facilities all become potential attachment sites for them. The attachment and growth of marine organisms on surfaces is known as biofouling. Marine biofouling will cause serious hazards such as increasing th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N45/00C09D5/16A01P7/00A01P13/00
Inventor 冯丹青柯才焕卢昌义
Owner XIAMEN UNIV
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