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Method for extracting mulberry leaf total alkaloid from mulberry leaf extract liquid

A technology of mulberry leaf extract and total alkaloids, applied in the directions of alkaloids, alkaloids, drug combinations, etc., can solve the problems of poor operability, cumbersome process, increased process steps, etc. The effect of high alkali transfer rate and simple process flow

Inactive Publication Date: 2009-07-29
BEIJING HERUN INNOVATION PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the test, it can be found that although the method can improve the purity and transfer rate of alkaloids and has certain progress, there are still serious defects: a large amount of toxic organic solvents such as methanol and ethanol are used, which affects the safety of production operations and final preparations. Sexuality brings hidden dangers; free mulberry leaf alkaloids under alkaline conditions need to have good solubility in ethanol solution, but the use of ethanol solution will bring resin monomer components styrene, di Residues of vinylbenzene, etc. affect the safety of the preparation; during operation, the resin needs to be poured out, and then refluxed with an organic solvent, the process is cumbersome and the operability is poor; at the same time, due to the use of ethanol in the process, it is also required to increase the ethanol treatment step in the pre-treatment of the resin. Ethanol will also be recovered, which increases the process steps and increases the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 1000g of mulberry leaf decoction pieces, decoct twice with water, 10 times the amount each time, decoct for 2 hours, combine the decoction liquid, centrifuge, adjust pH=3, centrifuge, and the supernatant is passed through cation exchange at 4 times the column volume / hour Resin (001×7, hydrogen type, diameter-to-height ratio 1:8), washed with water until the effluent is colorless, then eluted with 15% hydrochloric acid solution, the elution rate is 2 times the column volume / hour, until the alkaloid test is negative (The effluent was added dropwise with 10% silicotungstic acid without precipitation), the eluate was collected, neutralized to neutral with sodium hydroxide, desalted, and the filtrate was concentrated and dried to obtain the total alkaloids of mulberry leaves.

Embodiment 2

[0037] Get 1000g of mulberry leaf decoction pieces, add pH=5 acidic water percolation, combine the percolation liquid and add water and dilute to 10000ml, centrifuge, adjust pH=1, centrifuge, supernatant passes through cation exchange resin (Doulex 50, sodium type, diameter-to-height ratio 1:5), wash with water until the effluent is colorless, then elute with 5% hydrochloric acid solution, the elution rate is 4 times the column volume / hour, after elution is 2 times the column volume, soak for 5 hours , and then eluted until the alkaloid test was negative (the effluent was added dropwise with 10% silicotungstic acid without precipitation), the eluate was collected, neutralized to neutral with calcium hydroxide, desalted, the filtrate was concentrated and dried to obtain the total biomass of mulberry leaves alkali.

Embodiment 3

[0039] Take 5 kg of mulberry leaf decoction pieces, add 70% ethanol to ultrasonically extract twice, add 5 times the amount each time, ultrasonically for 0.5 hours, combine the extracts, centrifuge, recover ethanol, add water to 5000ml, adjust pH = 2, centrifuge, and the supernatant with 2 times the column volume / hour, pass through the upper cation exchange resin (001×10, sodium type, diameter-to-height ratio 1:7), wash with water until the effluent is colorless, and then wash with 1% hydrochloric acid solution (containing 1% sodium chloride) Elution, the elution rate is 5 times the column volume / hour, until the alkaloid test is negative (10% silicotungstic acid is added dropwise to the effluent without precipitation), the eluate is collected, neutralized to neutral with sodium hydroxide, and desalted , the filtrate was concentrated and dried to obtain the total alkaloids of mulberry leaves.

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Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely mulberry leaf, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the mulberry leaf; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; collecting eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and obtaining the mulberry leaf total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the mulberry leaf total alkaloid from the extracting solution of the mulberry leaf.

Description

technical field [0001] The invention relates to a method for extracting and separating active ingredients of traditional Chinese medicine, in particular to a method for extracting and separating total alkaloids of mulberry leaves from traditional Chinese medicine mulberry leaf extract, belonging to the field of traditional Chinese medicine. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. The mulberry leaves are the dried leaves of Morus alba L., a plant of the family Moraceae, and should meet the provisions of the Chinese Pharmacopoeia 2005 edition under the item of mulberry leaves. Mulberry leaf is a commonly used folk Chinese herbal medicine, which has the functions of dispelling wind-heat, clearing lung and moistening dryness, calming liver and improving eyesight. Studies have shown that...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61P3/10C07G5/00A61K127/00
Inventor 李蓉朴美善
Owner BEIJING HERUN INNOVATION PHARMA TECH DEV
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