Bacillus subtilis neutral phytase phyC gene

Abstract

The invention relates to neutral phytase gene phyC which comes from microorganism, has enzymolysis phytic acid, phytate and a complex compound thereof and releases mineral nutrition element activity of phosphorus and the like, the optimal pH in enzymatic reaction of an expression product thereof is neutral and is suitable for genetic transformation researches on crops such as mulberry tree, corn and rice and other crops with neutral harvested products. Therefore, with the phyC gene transferred to the mulberry tree, the optimal pH in enzymatic reaction of the expression product is consistent with the pH of the mulberry leaf, in addition, the enzymatic reaction of the expression product of the phyC gene can carry out enzymolysis on the phytic acid, the phytate and the complex compound thereof to release the phosphorus, contributes to absorption and application of the phosphorus by the mulberry tree, reduces fertilization of phosphate fertilizer and pollution of the phosphate on the environment, promotes growth of the mulberry tree; in addition, neutral phytase gene phyC of the invention features relatively high enzymatic activity, approximately 10% higher than the activity of the phytase of the same kind according to measurement.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a neutral phytase gene phyC derived from microorganisms which has the activity of enzymatically decomposing phytic acid, phytate and complexes thereof, and releasing mineral nutrients such as phosphorus. Background technique [0002] Phytic acid (phytic acid), also known as myo-inositol hexakisphosphate, can be used as storage phosphorus for the plant itself, and is gradually hydrolyzed and utilized when the seeds germinate. But most of it exists in the form of double salt, which is an anti-nutritional factor. Like trypsinogen inhibitors, prothrombin, saponin, tannins, oxalic acid, glucosinolates, gossypol, anti-fat-soluble vitamins, anti-B vitamins and other anti-nutrients, it is used in feed by livestock After the poultry eats, it affects the digestion and absorption of mineral nutrients and protein by livestock and poultry. And non-ruminants are physiologically unavailable for p...

AI Technical Summary

Problems solved by technology

[0004] The report that obtains phytase from microorganism has following three kinds at present: (1) mainly derive from the acid phytase (phyA) of Aspergillus niger, it has higher enzymatic activity, and the pH value effective range of its enzymatic reaction is in Between 4.5 and 6.0, suitable for monogastric animals and a few fish with acidic gastric pH, not suitable for carps with neutral digestive tract, which limits the application range of acid phytase

(2) Neutral phytase (phyC) derived from Bacillus, which has higher thermal stability, causes less loss of enzyme activity during feed pelleting or puffing, and the optimum pH of the enzymatic reaction is 7.0~ 7.5, suitable for carps feed, can make up for the deficiency of acid phytase, but its enzyme activity is not high