Method for extracting high-purity scutellarin from breviscpini

Abstract

The invention relates to a method for extracting high-purity scutellarin from breviscapin. The method comprises the following steps: the breviscapin raw material is extracted by using organic solvent, the filtrate is added with clarifying agent for coagulation and clarifying, the coagulated and clarified filtrate is absorbed by macroporous absorbent resin and is eluted with water, the water eluent is concentrated through a reverse osmosis membrane or nanofiltration membrane, the concentrated solution is added with the organic solvent for standing and precipitation, the sediment is filtered and collected, the aqueous solution of the organic solvent is added and stirred to be suspendible, and after standing a night, the sediment is filtered, collected and washed to be near-neutral with water to obtain the high-purity scutellarin after drying. The alkali is not used in the entire process, the clarifier is used for removing the water insoluble matter, the impurity removal is carried out by using the macroporous absorbent resin, the concentration is carried out by using the reverse osmosis membrane or the nanofiltration membrane, the scutellarin plant salt is prevented from being heated and degraded in water, the content of the prepared scutellarin can reach above 97 percent, the quality is stable, the process is simple and the pollution is little.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective plant components, in particular to a method for extracting and separating high-purity scutellarin from scutellaria scutellaria. Background technique [0002] Erigeron breviscapus (vant) Hard, -Mass is the dry whole herb of Compositae plant Erigeron breviscapus (vant) Hard, -Mass, also known as Erigeron breviscapus, East chrysanthemum. Distributed in Yunnan, Sichuan, Guizhou, Guangxi, Hunan and other places. Cold in nature, slightly bitter, has the effects of dispelling cold and relieving the exterior, expelling wind and dampness, promoting blood circulation and removing blood stasis, dredging meridian and activating collaterals, reducing inflammation and relieving pain. Once included in the "Chinese Pharmacopoeia" 1977 edition. Breviscapine is an active ingredient of flavonoids extracted from breviscapine herb, mainly scutellarin, also known as scutellarin, chemica...

AI Technical Summary

Problems solved by technology

Breviscapine is an active ingredient of flavonoids extracted from breviscapine herb, mainly scutellarin, also known as scutellarin, chemically named 4', 5, 6-trihydroxyflavone-7-O-glucose Aldehydroside, the scutellarin raw material drug currently purchased from the market is analyzed by HPLC, and the content of scutellarin is between 80-90%. Its various preparations are clinically used to treat cardiovascular and cerebrovascular diseases, with definite curative effect However, the stability of its injection is poor, and its ingredients other than breviscapine are not clear, which increases the uncontrollable safety of breviscapine injection, so it is of great significance to extract and separate high-purity breviscapine from breviscapine

[0003] In order to solve the technical problem of low content of scutellarin in scutellarin for a long time, patent applications 200410062573.3; 200410040182.1; 200410040352.6; 200410079583.8; The content of scutellarin prepared by the technology is high, reaching more than 98%. Patent applications 200710066155.5 and 200710065713.6 use macroporous adsorption resin to purify scutellarin, but the above patents are all refined with commercially available scutellarin and require a large amount of However, there is no relevant literature report on the extraction and separation process of high-purity scutellarin with stable quality directly extracted from scutellaria breviscapine