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Method for inducing and extracting metallothionein of the liver of sturgeon

A metallothionein and sturgeon technology is applied in the fields of purification and preparation, induction and extraction of sturgeon liver metallothionein products, and can solve the problems of troublesome injection operation, toxicity of inorganic inducers, and high extraction cost, and achieves convenient operation, The effect of reducing cost and improving purity

Inactive Publication Date: 2009-09-09
蓝泽桥
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

This traditional method has disadvantages such as raw material source constraints, inorganic inducer toxicity, large side effects, troublesome injection operation, high extraction cost, low yield, poor quality and purity, etc.

Method used

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  • Method for inducing and extracting metallothionein of the liver of sturgeon

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Experimental program
Comparison scheme
Effect test

Embodiment

[0018] 1. Breed 116kg commercial sturgeon in a 3.5m 3 2.5 kg of sturgeon special pellet feed added with 4-12 g of inducer zinc acetate was fed daily in a factory-like circulating pool, and the dose of zinc acetate added was increased day by day. The feed was continued for 10 days, and the liver was slaughtered for later use.

[0019] 2. Remove the gallbladder and fat from the liver, cut it into pieces and weigh 1680g.

[0020] 3. Preparation of eluent: 10 kg of 0.01 mol / L Tris-Hcl. solution with pH 8.6. Tris is trihydroxymethylaminomethane.

[0021] 4. Prepare liver homogenate buffer: take 5000ml of eluate, 5015ml of absolute ethanol, and 10415ml of 400ml of chloroform and mix them evenly.

[0022] 5. Pour 1680ml of liver homogenization buffer into the shredded liver tissue, mix well, and put it into an electric homogenizer for homogenization.

[0023] 6. Add 5000ml of liver homogenate buffer to the liver slurry, mix well, transfer to a low-temperature ultracentrifuge, and ...

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Abstract

The invention belongs to the bio-pharmaceuticals field, in particular to a method for preparing metallothionein by taking the liver of sturgeon as raw material. The method mainly comprises steps: sturgeons fed with feed added with inducer-zinc acetate by humans are slaughtered to take livers thereof; liver homogenate buffer solution is used for homogenizing and centrifuging, glucan and iminodiacetic acid mixture chromatography column chromatography collects chromatography liquid, and then the metallothionein is obtained by vacuum cooling drying. The liver of sturgeon is rich in metallothionein and is 100 times of liver content of other animals. The method is used for extracting the metallothionein, which can reduce the cost by 30%, increases yield by 40% and quality, and the purity the metallothionein reaches more than 99%. The metallothionein is expensive, has obvious physiological function and extensive use. Extracting metallothionein by using the liver of sturgeon has wide market prospect.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to methods for inducing, extracting and purifying a sturgeon liver metallothionein product. Background technique [0002] In 1957, when Marghes and Vallee were studying the biological effects of metals, they isolated a new protein from animal organs, which was rich in sulfhydryl groups and could chelate a large amount of metal ions. This substance was called metallothionein (MT for short). MT is an important, singular, active substance with strong physiological functions, known as one of the patron saints of life. At present, scientific and technological workers at home and abroad use animal rabbits, pigs, mice and other livers as raw materials, and use inorganic compounds such as Zn, Cu, Se, Cd, Pb and other metal elements such as ZnCl 2 , ZnSO 4 etc. as an inducer, using intramuscular or intraperitoneal injection to induce and stimulate the body, producing a large a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/825C07K1/36
Inventor 兰泽桥曹玉广钱名全刘艾平李义勇
Owner 蓝泽桥
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