Method for preparing ready-to-use tea fungus starter
A kombucha and starter technology, applied in the direction of microbial-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the problem of inconvenient recovery and cleaning of bacterial film, affecting normal fermentation, and difficult control And other issues
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[0026] Specific embodiments: The specific embodiments of the present invention will be described in detail below:
[0027] 1. Bacteria:
[0028] Acetic acid bacteria: Acetobacter acetobacter subsp. xylinum; other similar acetic acid bacteria such as Gluconobacter, Acetobacter ketobacillus, Acetobacter suboxyoxida, Acetobacter pasteurum, etc. can also be used.
[0029] Lactobacillus: Lactobacillus bulgaricus, Bifidobacterium; Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and other similar lactic acid bacteria can also be used.
[0030] Yeast: Pasteur yeast, Saccharomyces cerevisiae; other similar yeasts can also be used.
[0031] 2. Preparation of culture medium:
[0032] Yeast liquid culture medium:
[0033] The existing potato starch medium and malt extract medium can be used, or the potato starch medium prepared according to the following method: wash and peel the potatoes, cut them into small pieces, weigh 200g, and add 200g of water , Boil for 30mi...
Example Embodiment
[0038] Example 1:
[0039] Put Pasteur's yeast and Saccharomyces cerevisiae into the above-mentioned yeast liquid culture medium, culture in a static state at a temperature of 22°C, culture for 2 days, harvest the cells by centrifugation and wash twice with sterile water;
[0040] The Acetobacter xylinum subsp. xylinum was connected to the above-mentioned acetic acid bacteria liquid medium, a rotary shaker, a rotation speed of 220 rpm; a temperature of 30 °C, culture for 2 days, centrifugation to harvest the bacteria and wash twice with sterile water;
[0041] Put Lactobacillus bulgaricus and Bifidobacterium into the above-mentioned lactic acid bacteria liquid culture medium, rotating shaker, rotating speed 220rpm; temperature 37℃, culturing for 2 days, centrifugal harvesting bacteria and washing twice with sterile water;
[0042] Preparation of kombucha culture solution: take 150g of sucrose, 20g of Pu'er tea, and 830g of water, boil the water, cool the boiling water to 85℃~90℃, ...
Example Embodiment
[0050] Example 2:
[0051] Put Pasteur's yeast into the above-mentioned yeast liquid medium, culture it in a static state at a temperature of 20°C, cultivate for 1 day, harvest the bacteria by centrifugation and wash once with sterile water;
[0052] Put Acetobacter gluconobacter and Acetobacter ketobacillus into the above-mentioned acetic acid bacteria liquid medium, a rotary shaker, a rotation speed of 220 rpm; a temperature of 25 °C, culture for 1 day, centrifugation to harvest the bacteria and wash once with sterile water;
[0053] Connect Streptococcus thermophilus and Lactobacillus acidophilus into the above-mentioned lactic acid bacteria liquid culture medium, a rotary shaker, a rotation speed of 220 rpm; a temperature of 35 °C, culture for 1 day, centrifugation to harvest the bacteria and wash once with sterile water;
[0054] Preparation of kombucha culture solution: Take 50g of sucrose, 2g of Longjing tea, and 948g of water, boil the water, and when the boiling water is ...
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