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Cultivating method of tuckahoe

A method for cultivating tuckahoe, the technology of tuckahoe, is applied in the fields of botanical equipment and method, fertilizer mixture, horticulture, etc., which can solve the problems of long production cycle, skin, mud, sand, and low yield of tuckahoe products, so as to reduce the dosage, Poria cocos products have excellent and stable quality and the effect of accelerating growth rate

Inactive Publication Date: 2009-11-18
贵州昌昊中药发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the market demand for Poria cocos has been increasing day by day. At present, traditional cultivation is mainly carried out through the introduction of mycelia, old section wood and fresh Poria cocos. Poria", "Jiwo Lingjiao" and Poria products with skin, mud, sand and other undesirable phenomena
Chinese Patent Publication No. CN1732761A, on February 15, 2006, disclosed "a method for cultivating Poria cocos", although the above problems have been solved to a certain extent, but there are problems such as low yield and long production cycle.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of cultivation method of Poria cocos, comprises the following steps:

[0022] (1) Obtaining pure strains

[0023] Select a young Poria cocos with thin skin, purplish red skin, full shape, many cracks, tight texture, white and fine flesh, rich milk, about 7 months of growth period, strong development and strong vitality as the strain. Peel off the epidermis, sterilize the surface with 70% ethanol, rinse it with sterile water, then dry it with sterile cloth or filter paper, light the alcohol lamp in the sterile inoculation box, and cut it with a sterile knife beside the flame , use a knife to dig the cross-section into an arc, dig twice, pick the size of wheat grains at the second digging place and connect them to the pure culture medium, put them in the greenhouse, and cultivate them at a temperature of 30°C until they are covered with bacteria. Silk, screened white and free of bacterial infection, thick and strong in the shape of fluff, vigorous and uniform grow...

Embodiment 2

[0035] A kind of cultivation method of Poria cocos, comprises the following steps:

[0036] (1) Obtaining pure strains

[0037] Select a young Poria cocos with thin skin, purplish red skin, full shape, many cracks, tight texture, white and fine flesh, rich milk, about 8 months of growth period, strong development and strong vitality as the strain. Peel off the epidermis, sterilize the surface with 75% ethanol, rinse it with sterile water, then dry it with sterile cloth or filter paper, light the alcohol lamp in the sterile inoculation box, and cut it with a sterile knife beside the flame , use a knife to dig the cross-section into an arc shape, dig twice, pick the size of wheat grains from the second digging place and connect them to the pure culture medium, put them in the greenhouse, and cultivate them at 25°C until they are covered with bacteria. Silk, screened white and free of bacterial infection, thick and strong in the shape of fluff, vigorous and uniform growth, secre...

Embodiment 3

[0048] A kind of cultivation method of Poria cocos, comprises the following steps:

[0049] (1) Obtaining pure strains

[0050] With embodiment 1;

[0051] (2) Cultivation of the primary strain (mother species)

[0052] In the sterile inoculation box, insert the pure strains into the pre-prepared primary strain culture medium according to the conventional inoculation method (the medium is potato-glucose medium, and its formula and preparation method are the same as the pure strain culture medium) When cultured at a temperature of 28°C, the mycelium can cover the slope within 2 days, and the first-grade mother species can be obtained; one test tube of pure strains can be connected with 6 to 8 first-grade strains (mother species). In order to expand the quantity of the mother species, the obtained mother species can be transferred to the transfer mother species medium (this medium is a potato-glucose medium, and its formula and preparation method are the same level as the bact...

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Abstract

The invention discloses a cultivation method of tuckahoe, which comprises the steps of: (1) firstly, the epidermis of the tuckahoe is cut; the tuckahoe is superficially sterilized by 70 to 75% of ethanol and washed with sterile water; the water content of the tuckahoe is blotted up by disinfection cloth or a filter paper; in an aseptic inoculation box, an alcohol burner is lightened, the processed tuckahoe is sliced off by an abacterial knife near fire and the cross section is scraped into be cambered and successively scraped for twice; on the second scraping position, blocks like wheat size are connected on a pure strain culture medium, placed in a greenhouse, and cultured in the temperature of 25 to 30 DEG C till mycelial overgrows; the blocks which are white, are not infected by mixed bacterium, are villous, dense and craggy, vigorously and uniformly grow, secretes white milk pearl mycelium are pure strains; (2) a primary strain cultivation is carried out; (3) a second class strain cultivation is carried out; (4) a third class strain cultivation is carried out. The method is characterized by short production period of mycelium, excellent and stable tuckahoe quality and high yield.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for cultivating poria cocos. Background technique [0002] Poria cocos is a commonly used traditional Chinese medicine. It is a species recorded in the 2005 edition of the "Pharmacopoeia of the People's Republic of China". This product is the dried sclerotia of Polyporaceae fungus Poria cocos (Schw.) Wolf. Nature and flavor are sweet, light, flat, and return to the heart, lung, spleen, and kidney meridians. It has diuresis and dampness expelling, invigorating the spleen and calming the heart. For edema, oliguria, phlegm retention, dizziness and palpitations, deficiency of the spleen and lack of food, loose stools and diarrhea, restlessness, palpitations and insomnia, Poria cocos has a wide range of use value in Chinese medicine clinics and people's lives. [0003] In recent years, the market demand for Poria cocos has been increasing day by day. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 郑建立杨光明屠伦建兰才武邓忠
Owner 贵州昌昊中药发展有限公司
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