Microbial fertilizer-plant synbiotic composition preparation and method for preparing symbiotic preparation
A microbial fertilizer and synbiotic technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fertilizer mixtures, etc., can solve the problems of research and application limited to animals, single function, and increase fertilizer efficiency, so as to overcome the obstacles of continuous cropping , increase soil nutrients, improve the effect of promoting growth
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Embodiment 1
[0071] A microbial fertilizer-plant synbiotic composition preparation, the preparation consists of 85-99 parts of probiotics, 0.05-3.5 parts of prebiotics, and 1.5-10 parts of stabilizers to form plant synbiotics, wherein the probiotics The ratio range of the composition of the plant synbiotics with the prebiotics is 30-60 parts: 1 part.
[0072] A preparation method of synbiotics preparation, carried out according to the following steps:
[0073] 1) Fermentation of probiotics
[0074] ①. Seed production: Inoculate different bacterial strains on different sterilized plate media, culture at a constant temperature of 32°C to 37°C for 48 hours, and use 10 to 15mL of sterile water to inoculate the bacteria on the colony on a sterile operating table. The bacteria are fully suspended, transferred into a sterilized eggplant-shaped bottle, incubated at a constant temperature of 32°C to 37°C for 48 hours, and treated at a temperature of 80°C for 10 minutes, and used for fermenter inoc...
Embodiment 2
[0088] A microbial fertilizer-plant synbiotic composition preparation, the preparation is composed of 90-95 parts of probiotics, 0.05-2.0 parts of prebiotics, and 3-6 parts of stabilizers to form plant synbiotics, wherein the probiotics The ratio range of forming the plant synbiotics with the prebiotics is 45-60 parts: 1 part.
[0089] A preparation method of synbiotics preparation, carried out according to the following steps:
[0090] 1) Fermentation of probiotics
[0091] ①. Seed production: Insert different strains into different sterilized plate media, culture at a constant temperature of 35°C for 48 hours, and fully suspend the bacteria on the colony with 15 mL of sterile water on the sterile operating table , transferred to a sterilized eggplant-shaped bottle, cultivated at a constant temperature of 35°C for 48 hours, and treated at a temperature of 80°C for 10 minutes, and used for fermenter inoculation;
[0092] ②. Fermentation: Fermentation tanks at all levels prep...
Embodiment 3
[0105] Bacillus subtilis (Bacilus subtis) culture
[0106] Bacillus subtilis slant medium: peptone 10g, beef extract 5g, NaCl 5g, agar 20g, water 1000ml, pH7.2.
[0107] Bacillus subtilis liquid medium: bean curd 1000g, sugar 1000g, CaCO3 500g, NaCl 30g, kCl 30g, MgSO4 30g, (NH4)2 SO4 50g, MnSO4 3g, FeSO4 3g, water 100L.
[0108] Bacillus subtilis culture characteristics: the highest growth temperature is 40-50°C, the optimum growth temperature is 33-37°C, the optimum fermentation temperature is 29-33°C, and the optimum pH is 5.5-8.5.
[0109] Morphological characteristics of Bacillus subtilis:
[0110] Gram-positive bacilli, about 0.6-0.8 x 1.5-3.0 microns in size. Cells grown on glucose agar medium stained homogeneously and motilely. The spores are elliptical, mesogenic or partially mesogenic, not inflated or slightly inflated. The colonies are round, with dark, rough surface and irregular edges.
[0111] Liquid culture conditions in the fermenter: the filling factor is...
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