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Microbial fertilizer-plant synbiotic composition preparation and method for preparing symbiotic preparation

A microbial fertilizer and synbiotic technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fertilizer mixtures, etc., can solve the problems of research and application limited to animals, single function, and increase fertilizer efficiency, so as to overcome the obstacles of continuous cropping , increase soil nutrients, improve the effect of promoting growth

Active Publication Date: 2012-07-04
渭南德龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] The present invention comprehensively considers the micro-ecology of the plant rhizosphere and the defense of the plant itself, proposes the concept of plant synbiotics, proposes a plant synbiotic composition that increases fertilizer efficiency, and solves the problem of the existence of microbial fertilizers or a single strain of bacteria in the past. , single function, or only use microorganisms to fix nitrogen, phosphorus, potassium, and competitive inhibition.
[0023] At present, a small amount of oligosaccharide-probiotic combination products have appeared in the domestic and foreign markets, but the research and application are limited to animals.

Method used

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  • Microbial fertilizer-plant synbiotic composition preparation and method for preparing symbiotic preparation
  • Microbial fertilizer-plant synbiotic composition preparation and method for preparing symbiotic preparation
  • Microbial fertilizer-plant synbiotic composition preparation and method for preparing symbiotic preparation

Examples

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Effect test

Embodiment 1

[0071] A microbial fertilizer-plant synbiotic composition preparation, the preparation consists of 85-99 parts of probiotics, 0.05-3.5 parts of prebiotics, and 1.5-10 parts of stabilizers to form plant synbiotics, wherein the probiotics The ratio range of the composition of the plant synbiotics with the prebiotics is 30-60 parts: 1 part.

[0072] A preparation method of synbiotics preparation, carried out according to the following steps:

[0073] 1) Fermentation of probiotics

[0074] ①. Seed production: Inoculate different bacterial strains on different sterilized plate media, culture at a constant temperature of 32°C to 37°C for 48 hours, and use 10 to 15mL of sterile water to inoculate the bacteria on the colony on a sterile operating table. The bacteria are fully suspended, transferred into a sterilized eggplant-shaped bottle, incubated at a constant temperature of 32°C to 37°C for 48 hours, and treated at a temperature of 80°C for 10 minutes, and used for fermenter inoc...

Embodiment 2

[0088] A microbial fertilizer-plant synbiotic composition preparation, the preparation is composed of 90-95 parts of probiotics, 0.05-2.0 parts of prebiotics, and 3-6 parts of stabilizers to form plant synbiotics, wherein the probiotics The ratio range of forming the plant synbiotics with the prebiotics is 45-60 parts: 1 part.

[0089] A preparation method of synbiotics preparation, carried out according to the following steps:

[0090] 1) Fermentation of probiotics

[0091] ①. Seed production: Insert different strains into different sterilized plate media, culture at a constant temperature of 35°C for 48 hours, and fully suspend the bacteria on the colony with 15 mL of sterile water on the sterile operating table , transferred to a sterilized eggplant-shaped bottle, cultivated at a constant temperature of 35°C for 48 hours, and treated at a temperature of 80°C for 10 minutes, and used for fermenter inoculation;

[0092] ②. Fermentation: Fermentation tanks at all levels prep...

Embodiment 3

[0105] Bacillus subtilis (Bacilus subtis) culture

[0106] Bacillus subtilis slant medium: peptone 10g, beef extract 5g, NaCl 5g, agar 20g, water 1000ml, pH7.2.

[0107] Bacillus subtilis liquid medium: bean curd 1000g, sugar 1000g, CaCO3 500g, NaCl 30g, kCl 30g, MgSO4 30g, (NH4)2 SO4 50g, MnSO4 3g, FeSO4 3g, water 100L.

[0108] Bacillus subtilis culture characteristics: the highest growth temperature is 40-50°C, the optimum growth temperature is 33-37°C, the optimum fermentation temperature is 29-33°C, and the optimum pH is 5.5-8.5.

[0109] Morphological characteristics of Bacillus subtilis:

[0110] Gram-positive bacilli, about 0.6-0.8 x 1.5-3.0 microns in size. Cells grown on glucose agar medium stained homogeneously and motilely. The spores are elliptical, mesogenic or partially mesogenic, not inflated or slightly inflated. The colonies are round, with dark, rough surface and irregular edges.

[0111] Liquid culture conditions in the fermenter: the filling factor is...

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Abstract

The invention discloses a microbial fertilizer-plant synbiotic composition preparation and a method for preparing the synbiotic preparation. In the composition preparation, the plant synbiotic consists of probiotics, prebiotics and stabilizer; and bacillus forms zymotic fluid. The method for preparing the synbiotic preparation comprises the following steps: fermenting the probiotics; regulating bacteria amount; and preparing and homogenizing to obtain the product. Azotobacter, pralidoxime iodide and potassium bacteria in the preparation can increase soil nutrition, improve the fertilizer utilization rate and soil granule structures, promote the air permeability, water-retaining property and drainage, and overcome the defect of sunlight greenhouse continuous cropping; oligosaccharide prebiotics in the preparation can stimulate crops to improve resistance to pathogenic microorganisms, and promotes permanent planting and proliferation of the probiotics; the preparation can inhibit toxicity generated by bacteria, start self immunity system, eliminate diseases and remove evils, reduce bactericidal pesticide, has cold resistance, heat resistance, drought resistance, waterlogging resistance, saline and alkaline resistance, fertilizer damage resistance, chemical damage resistance and nutrition imbalance resistance, can promote plant root elongation and good development of capillary roots, and promote blooming and fruit yielding.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a preparation method of a microbial fertilizer-plant synbiotic composition and a synbiotic preparation applied in the field of agricultural production. Background technique [0002] With the continuous increase in the use of chemical synthetic substances such as pesticides, veterinary drugs, feed additives, fertilizers, and hormones, the number of agricultural products has increased rapidly, but at the same time it has inevitably caused serious pollution to the agricultural ecological environment and agricultural products. There is a potential threat to people's health, and directly affects the international competitiveness of agricultural products and export earnings. [0003] Most countries in the world, especially some developed countries, allow the use of auxin and hormones to decrease year by year, and the requirements for pesticide and fertilizer residues in crops are a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08C05G3/00C05G3/04C12N1/20C12R1/065C12R1/07C12R1/11C12R1/085C12R1/125C05G3/80
Inventor 刘甲启徐泽平芦晓飞王宝琴杨传伦王秀芝张心青苏亚平戴荣华门振
Owner 渭南德龙生物科技有限公司
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