Tissue culture method of pinctada fucata

A Hepu pearl oyster and tissue culture technology, applied in the field of in vitro culture of tissue cells, can solve the problems of poor cell adhesion, small number of migration, easy to cause pollution, etc., achieve good sterilization effect and protect from damage , easy-to-operate effect

Inactive Publication Date: 2009-12-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, there are a large number of bacteria, fungi, protozoa, etc. on the tissue surface of Hepu pearl oyster, which is very easy to cause pollution during the tissu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The culture of embodiment 1 mantle tissue cell

[0025] (1) Mantle collection: take Hepu pinnacle oyster, blot the surface of the shell with absorbent paper, wipe the surface of the shell with medical alcohol for disinfection, and cut off the edge of the shell on the adductor muscle side. In the aseptic operating table, a scalpel was inserted into the shell body to cut the adductor muscle, and the mantle tissue was taken. The removed tissue was infiltrated in tissue balanced salt solution until sufficient tissue was collected.

[0026] (2) Disinfection and cleaning: use sterilized filter paper to remove the mucus on the surface of the mantle tissue. Soak the mantle tissue in tissue disinfectant for 20 minutes, shake it several times during the period, then transfer the mantle tissue to tissue balanced salt solution, change the solution 8 times, and clean the mucus and residual antibiotics on the surface of the mantle tissue.

[0027] (3) Tissue culture: use ophthalmic...

Embodiment 2

[0028] The cultivation of embodiment 2 pearl sac tissue cells

[0029] (1) Materials for pearl sacs: Hepu mother-of-pearl oyster was taken, the surface of the shell was blotted dry with absorbent paper, the surface of the shell was wiped with medical alcohol for disinfection, and the edge of the shell on the side of the adductor muscle was cut off. Insert the adductor muscle into the shell with a scalpel in the aseptic operating table (the adductor muscle should be cut along the surface of the shell to avoid contamination of visceral tissue cells), and take out the pearl sac. The removed tissue was infiltrated in tissue balanced salt solution until sufficient pearl sacs were collected.

[0030] (2) Disinfection and cleaning: use sterilized filter paper to remove the mucus on the surface of the pearl sac tissue. Soak the pearl sac tissue in the tissue disinfectant solution for 20 minutes, shake it several times during the period, then transfer the pearl sac tissue to the tissu...

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Abstract

The invention relates to a tissue culture method of pinctada fucata, and belongs to tissue cell culture technology of the pinctada fucata. The method mainly comprises the following steps: (1) tissue mass taking, during which, carrying out surface sterilization of the pinctada fucata and cutting an adductor muscle in a sterile operating desk to take out a target tissue; (2) sterilization and cleaning, during which, wiping the mucus on the surface of the tissue by paper and transferring the tissue to tissue balanced salt solution after sterilization to clean surface mucus and residual antibiotics; and (3) tissue culture, during which, cutting the tissue mass into small pieces, putting the small pieces in a tissue culture dish and adding culture medium for culture. The tissue culture method has wide application range and better sterilization effect, and can protect tissue cell from being damaged; moreover, during tissue mass culture, the method ensures excellent adherence effect, vigorous cell migration, longer longevity in vitro and easy operation. The culture medium adopted by the method is more suitable for the culture of tissue cells of marine shellfish.

Description

technical field [0001] The invention relates to tissue cell culture technology, in particular to a method for in vitro culture of tissue cells of pearl oyster---Hepu pinnacle oyster. Background technique [0002] Pinctada fucata Martenssi, also known as Pinctada fucata Martenssi, belongs to the phylum Mollusca, the class Bivalvia, the order Pterioida, the family Pteriidae, and the genus Pinctada ( Pinctada), mainly distributed in Japan and the South my country Sea of ​​China, is the main shellfish used in the production of seawater pearls in the world at present, and its pearl production accounts for more than 85% of the world's seawater pearl production, and it is also the main seawater pearl culture shellfish in my country. However, with the prolongation of the history of shell farming and the development of high-density, intensive and industrialized production, as well as the deteriorating environment of the farming conditions, breeding diseases have become an important fa...

Claims

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Application Information

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IPC IPC(8): C12N5/06
Inventor 张荣庆谢莉萍李琪上官俊龙刘晓军周玉娟
Owner TSINGHUA UNIV
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