Tissue culture method of pinctada fucata

Abstract

The invention relates to a tissue culture method of pinctada fucata, and belongs to tissue cell culture technology of the pinctada fucata. The method mainly comprises the following steps: (1) tissue mass taking, during which, carrying out surface sterilization of the pinctada fucata and cutting an adductor muscle in a sterile operating desk to take out a target tissue; (2) sterilization and cleaning, during which, wiping the mucus on the surface of the tissue by paper and transferring the tissue to tissue balanced salt solution after sterilization to clean surface mucus and residual antibiotics; and (3) tissue culture, during which, cutting the tissue mass into small pieces, putting the small pieces in a tissue culture dish and adding culture medium for culture. The tissue culture method has wide application range and better sterilization effect, and can protect tissue cell from being damaged; moreover, during tissue mass culture, the method ensures excellent adherence effect, vigorous cell migration, longer longevity in vitro and easy operation. The culture medium adopted by the method is more suitable for the culture of tissue cells of marine shellfish.

Description

technical field [0001] The invention relates to tissue cell culture technology, in particular to a method for in vitro culture of tissue cells of pearl oyster---Hepu pinnacle oyster. Background technique [0002] Pinctada fucata Martenssi, also known as Pinctada fucata Martenssi, belongs to the phylum Mollusca, the class Bivalvia, the order Pterioida, the family Pteriidae, and the genus Pinctada ( Pinctada), mainly distributed in Japan and the South my country Sea of ​​China, is the main shellfish used in the production of seawater pearls in the world at present, and its pearl production accounts for more than 85% of the world's seawater pearl production, and it is also the main seawater pearl culture shellfish in my country. However, with the prolongation of the history of shell farming and the development of high-density, intensive and industrialized production, as well as the deteriorating environment of the farming conditions, breeding diseases have become an important fa...

AI Technical Summary

Problems solved by technology

On the one hand, there are a large number of bacteria, fungi, protozoa, etc. on the tissue surface of Hepu pearl oyster, which is very easy to cause pollution during the tissue culture process; on the other hand, the existing tissue culture technology of pearl oyster has poor cell adhesion and slow growth. , the number of emigration is small, etc.