Gene for increasing plant drought resistance and salt resistance and enconding product and application thereof
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A genetic and salt-tolerant technology, applied in the direction of plant genetic improvement, plant products, angiosperms/flowering plants, etc., can solve the problems of litchi preservation, storage and transportation difficulties, peeling browning, and affecting the development of litchi industry.
Inactive Publication Date: 2012-10-17
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +2
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However, the preservation, storage and transportation of litchi are difficult, especially the browning of litchi skin after harvesting is serious, which reduces the commodity value of litchi and affects the development of litchi industry.
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[0032] The present invention is described in detail below in conjunction with embodiment:
[0033] 1. Source of material: Litchi fruits are collected from
[0034] 2. Method:
[0035] (1) Litchi peel cDNA library construction: Fresh litchi fruit peeled immediately after harvest and stored in liquid nitrogen at -70°C, the total RNA of the peel was extracted by CTAB method, and the SMART of Clontech Company was used TM cDNA Library ConstructionKit, reverse transcribe cDNA according to the instructions, and construct a cDNA library.
[0036] (2) Microarray fabrication and differentially expressed gene screening: about 10,000 independent clones were randomly selected from the cDNA library, plasmid DNA was extracted and quantified, and the plasmid DNA was spotted on the chip using an automatic spotting machine. The cDNA was reverse-transcribed from the total RNA of litchi pericarp at 0h and 48h postharvest, respectively, and the total RNA of litchi pericarp at 0h and 48h after h...
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Abstract
The invention relates to a gene for increasing plant drought resistance and salt resistance and an encoding product and an application thereof and discloses a gene LaAsr1 which is related to litchi resistance and is one of nucleotide sequences as follows: 1) the DNA sequence in the <400>1 term in the sequence table; 2) protein in the <400>1 term with the amino acid residue sequence in the <400>2 term or the derived protein with the same activity of the amino acid residue sequence in the <400>2 term by replacing, deleting or adding one or more of amino acid residues to the amino acid residue sequence in pseudo column. The gene can be induced to express by water stress and salt stress and can increase the plant drought resistance and salt resistance, thus having an important theoretical and practical significance for cultivating good plant types and increasing anti-microbial effect of plants by regulating the gene.
Description
[technical field] [0001] The present invention relates to acquisition of litchi Asr (abscisic acid-, stress-, and ripening-induced gene, Asr) sequence, expression analysis, construction of plant expression vector and transformation of Arabidopsis thaliana to identify drought resistance and salt tolerance. [Background technique] [0002] Asr (abscisic acid-, stress-, and ripening-induced gene) (abscisic acid-, stress-, and ripening-induced gene) is a class of genes discovered in plants in recent years, since Iusem et al first isolated tomato Asr1 from tomato fruit (Iusem et al., 1993), many Asr cloned in different plants, such as: apricot (Mbeguie-A-Mbeguie et al., 1997), lily (Wang et al., 1998; Chang et al., 1999), Rice (Vaidyanathan et al., 1999), pear (Itai et al., 2000), grape (Cakir et al., 2003), ginkgo (Shen et al., 2005), etc. [0003] In recent years, the research on Asr has been in-depth, and there are many research reports on stress. According to literature repo...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
Inventor 金志强徐碧玉王家保董凤英刘菊华
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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