Gene for increasing plant drought resistance and salt resistance and enconding product and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A genetic and salt-tolerant technology, applied in the direction of plant genetic improvement, plant products, angiosperms/flowering plants, etc., can solve the problems of litchi preservation, storage and transportation difficulties, peeling browning, and affecting the development of litchi industry.
Inactive Publication Date: 2012-10-17
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +2
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, the preservation, storage and transportation of litchi are difficult, especially the browning of litchi skin after harvesting is serious, which reduces the commodity value of litchi and affects the development of litchi industry.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0032] The present invention is described in detail below in conjunction with embodiment:
[0033] 1. Source of material: Litchi fruits are collected from
[0034] 2. Method:
[0035] (1) Litchi peel cDNA library construction: Fresh litchi fruit peeled immediately after harvest and stored in liquid nitrogen at -70°C, the total RNA of the peel was extracted by CTAB method, and the SMART of Clontech Company was used TM cDNA Library ConstructionKit, reverse transcribe cDNA according to the instructions, and construct a cDNA library.
[0036] (2) Microarray fabrication and differentially expressed gene screening: about 10,000 independent clones were randomly selected from the cDNA library, plasmid DNA was extracted and quantified, and the plasmid DNA was spotted on the chip using an automatic spotting machine. The cDNA was reverse-transcribed from the total RNA of litchi pericarp at 0h and 48h postharvest, respectively, and the total RNA of litchi pericarp at 0h and 48h after h...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a gene for increasing plant drought resistance and salt resistance and an encoding product and an application thereof and discloses a gene LaAsr1 which is related to litchi resistance and is one of nucleotide sequences as follows: 1) the DNA sequence in the <400>1 term in the sequence table; 2) protein in the <400>1 term with the amino acid residue sequence in the <400>2 term or the derived protein with the same activity of the amino acid residue sequence in the <400>2 term by replacing, deleting or adding one or more of amino acid residues to the amino acid residue sequence in pseudo column. The gene can be induced to express by water stress and salt stress and can increase the plant drought resistance and salt resistance, thus having an important theoretical and practical significance for cultivating good plant types and increasing anti-microbial effect of plants by regulating the gene.
Description
[technical field] [0001] The present invention relates to acquisition of litchi Asr (abscisic acid-, stress-, and ripening-induced gene, Asr) sequence, expression analysis, construction of plant expression vector and transformation of Arabidopsis thaliana to identify drought resistance and salt tolerance. [Background technique] [0002] Asr (abscisic acid-, stress-, and ripening-induced gene) (abscisic acid-, stress-, and ripening-induced gene) is a class of genes discovered in plants in recent years, since Iusem et al first isolated tomato Asr1 from tomato fruit (Iusem et al., 1993), many Asr cloned in different plants, such as: apricot (Mbeguie-A-Mbeguie et al., 1997), lily (Wang et al., 1998; Chang et al., 1999), Rice (Vaidyanathan et al., 1999), pear (Itai et al., 2000), grape (Cakir et al., 2003), ginkgo (Shen et al., 2005), etc. [0003] In recent years, the research on Asr has been in-depth, and there are many research reports on stress. According to literature repo...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
Inventor 金志强徐碧玉王家保董凤英刘菊华
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI