Preparation method of paecilomyces lilacinus biological fertilizer
A technology of Paecilomyces lilacinus and biological fertilizer, which is applied in the field of preparation of Paecilomyces lilacinus biological fertilizers, can solve problems such as poor medicinal and fertilizer effects, pollute the environment, etc., and achieve significant economic benefits and environmental protection Benefit, promote plant growth, and the effect of simple and easy preparation
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Embodiment 1
[0033] (1) The preparation of the secondary liquid strain of Paecilomyces lilacinus, the specific operation steps are as follows:
[0034] A. Slope bacteria (solid)
[0035] Weigh 20 parts of potatoes, wash, peel and cut into small pieces, add 100 parts of water and boil for half an hour or high-pressure cook for 20 minutes, filter with gauze, add 2 parts of glucose and 2 parts of agar, dissolve them fully, filter with gauze while hot, and divide Put in a test tube, sterilize with 15 pounds of steam (121°C) for 20 minutes, take out the test tube and place it on an inclined plane, streak and inoculate Paecilomyces lilacinus NH-PL-03 strain in an incubator at 28°C for 8 days after cooling.
[0036] B. Secondary strains (liquid)
[0037] The formula of the culture medium is the same as that of the slant but without adding agar, and it is divided into Erlenmeyer flasks. After sterilizing and cooling, use an inoculation loop to pick up conidia from the Paecilomyces lilacinus prese...
Embodiment 2
[0045] Embodiment 2: the preparation of Paecilomyces lilacinus fertilizer
[0046] The steps different from Example 1 are as follows, and all the other unexplained steps are the same as Example 1:
[0047] Step (2) Prepare Paecilomyces lilacinus solid fermentation culture by thick-layer ventilated fermentation
[0048] 70 parts of decomposed farm fermented bedding materials, 15 parts of rice bran, 15 parts of cornstarch, add water after stirring, adjust the water content to 40%, and 8 parts of liquid strains for production in the inoculation step (1) (the above are all by weight parts), transferred to a solid fermentation tank, ventilated, fermented at 27°C, stirred once every 24 to 30 hours, and fermented for 9 days to obtain the solid fermentation culture of Paecilomyces lilacinus.
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