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Preparation method of paecilomyces lilacinus biological fertilizer

A technology of Paecilomyces lilacinus and biological fertilizer, which is applied in the field of preparation of Paecilomyces lilacinus biological fertilizers, can solve problems such as poor medicinal and fertilizer effects, pollute the environment, etc., and achieve significant economic benefits and environmental protection Benefit, promote plant growth, and the effect of simple and easy preparation

Inactive Publication Date: 2012-08-08
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a kind of preparation method of Paecilomyces lilacinus biofertilizer, solve the problems in the prior art such as simply mixing medicine and fertilizer, poor medicinal and fertilizer effects, polluting the environment, etc. series of problems, the preparation process is simple and easy, and the bacterial activity in the cultivated Paecilomyces lilacinus biological fertilizer is good. When solid fermentation is cultivated, the fertilizer is directly used as the culture medium, and microorganisms are used as medicinal components, so that the fertilizer of the present invention has The organic combination of medicine and fertilizer, the fertilizer and drug effects are very significant, and it has no pollution to the environment, and has significant economic benefits and environmental protection benefits

Method used

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  • Preparation method of paecilomyces lilacinus biological fertilizer
  • Preparation method of paecilomyces lilacinus biological fertilizer
  • Preparation method of paecilomyces lilacinus biological fertilizer

Examples

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Effect test

Embodiment 1

[0033] (1) The preparation of the secondary liquid strain of Paecilomyces lilacinus, the specific operation steps are as follows:

[0034] A. Slope bacteria (solid)

[0035] Weigh 20 parts of potatoes, wash, peel and cut into small pieces, add 100 parts of water and boil for half an hour or high-pressure cook for 20 minutes, filter with gauze, add 2 parts of glucose and 2 parts of agar, dissolve them fully, filter with gauze while hot, and divide Put in a test tube, sterilize with 15 pounds of steam (121°C) for 20 minutes, take out the test tube and place it on an inclined plane, streak and inoculate Paecilomyces lilacinus NH-PL-03 strain in an incubator at 28°C for 8 days after cooling.

[0036] B. Secondary strains (liquid)

[0037] The formula of the culture medium is the same as that of the slant but without adding agar, and it is divided into Erlenmeyer flasks. After sterilizing and cooling, use an inoculation loop to pick up conidia from the Paecilomyces lilacinus prese...

Embodiment 2

[0045] Embodiment 2: the preparation of Paecilomyces lilacinus fertilizer

[0046] The steps different from Example 1 are as follows, and all the other unexplained steps are the same as Example 1:

[0047] Step (2) Prepare Paecilomyces lilacinus solid fermentation culture by thick-layer ventilated fermentation

[0048] 70 parts of decomposed farm fermented bedding materials, 15 parts of rice bran, 15 parts of cornstarch, add water after stirring, adjust the water content to 40%, and 8 parts of liquid strains for production in the inoculation step (1) (the above are all by weight parts), transferred to a solid fermentation tank, ventilated, fermented at 27°C, stirred once every 24 to 30 hours, and fermented for 9 days to obtain the solid fermentation culture of Paecilomyces lilacinus.

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Abstract

The invention provides a preparation method of paecilomyces lilacinus biological fertilizer, belonging to the technical field of agrobiomechanics, and solving the problems in the prior art. The preparation method comprises the steps of: taking the paecilomyces lilacinus as bacterial strain, inoculating triangular flask liquid bacterial strain, and fermenting with czapek-dox medium to prepare liquid bacterial strain for production; taking decomposed or semi-decomposed fermenting bed padding materials in a culturing farm as basic medium, matching with accessorial carbon source and water, inoculating the liquid bacterial strain, and fermenting in a ventilation way in the depth layer of a solid fermenting pond to prepare paecilomyces lilacinus solid fermentation culture material; and drying the paecilomyces lilacinus solid fermentation culture material and grinding. The invention takes high-effective eelworm biocontrol bacteria paecilomyces lilacinus as the bacterial strain, takes the decomposed or semi-decomposed fermenting bed padding materials in the culturing farm as main raw materials, and ferments to prepare the paecilomyces lilacinus biological fertilizer to be taken as soil microorganism fertilizer which can prevent and cure the parasitic eelworm and various plant pathogenic bacteria, promote the plant growth, and reduce the pesticide residue in the soil.

Description

technical field [0001] The invention relates to a soil microbial fertilizer for both fertilizer and medicine, and more particularly relates to a preparation method of Paecilomyces lilacinus biological fertilizer. Background technique [0002] In traditional agricultural production, in order to increase production and income, farmers often use a large amount of chemical fertilizers and production hormones to promote crop growth, and at the same time use pesticides to control pests and diseases. The residues of toluene and other auxiliary additives have caused the soil structure, land fertility and plant ecology to be unbalanced, degraded and mutated in many places, causing intractable diseases of crops, especially vegetables and fruit trees, and the chemical pesticides used to control these diseases Increased the pollution of land and agricultural products. Under such circumstances, some manufacturers have begun to develop preparations with both medicinal and fertilizer effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G3/02C05F17/00C12N1/14A01N63/04A01P5/00A01P3/00C12R1/79C05G3/60
CPCY02W30/40
Inventor 刘波史怀
Owner 福建省农业科学院农业生物资源研究所
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