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Method for detecting single nucleotide polymorphism (SNP) of ox PRDM16 gene

A PRDM16, single nucleotide polymorphism technology, applied in the field of detecting the 212237th single nucleotide polymorphism of cattle PRDM16 gene

Inactive Publication Date: 2010-03-17
NORTHWEST A & F UNIV
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Problems solved by technology

There is no research on the genetic variation of PRDM16 gene in animals at home and abroad

Method used

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  • Method for detecting single nucleotide polymorphism (SNP) of ox PRDM16 gene
  • Method for detecting single nucleotide polymorphism (SNP) of ox PRDM16 gene
  • Method for detecting single nucleotide polymorphism (SNP) of ox PRDM16 gene

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Embodiment Construction

[0024] The present invention uses the PCR-RFLP method to detect the single nucleotide polymorphism that may result in a change in the conformation of the encoded protein due to the mutation of the missense codon at the 212237 site of the cattle PRDM16 gene, and the correlation analysis between the SNP and the traits shows that the site Point polymorphisms can become molecular breeding markers. The following will further describe the present invention in detail, which is an explanation of the present invention rather than a limitation.

[0025] a. Design of PCR primers for the region containing the ninth exon of the cattle PRDM16 gene

[0026] Taking the bovine (NC_007314.3) sequence published by NCBI as a reference, Primer 5.0 was used to design PCR primers capable of amplifying the region of the ninth exon of the cattle PRDM16 gene. The primer sequences are as follows:

[0027] Upstream primer: cctaccactc cgtgttccc 19;

[0028] Downstream primer: tcggctgcca atgctc 16;

[0...

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Abstract

The invention discloses a method for detecting the single nucleotide polymorphism (SNP) of an ox PRDM16 gene, which comprises the following steps: carrying out PCR amplification on the ox PRDM16 geneby adopting ox whole-genome DNA to be detected, which contains the PRDM16 gene, as a template and adopting a primer pair P as primers; digesting a PCR amplification product by restriction endonucleaseMspl and then carrying out polyacrylamide gel electrophoresis on an amplified fragment after enzyme digestion; and authenticating the 212237th site SNP of the ox PRDM16 gene according to a polyacrylamide gel electrophoresis result. Because the PRDM16 gene function relates to birth weight, daily gain and body weight and growth characteristics, the detecting method lays foundation for establishingthe relation of the SNP of the PRDM16 gene and the growth characteristics so as to be used for the marker assisted selection (MAS) of the growth characteristics of a Chinese ox and rapidly establishing an ox population with favorable genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the 212237th SNP of cattle PRDM16 gene. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Therefore, commonly referred to as SNPs include base substitutions, insertions, deletions, and changes in the copy number of repeated sequences. A SNP indicates that there is a nucleotide change at a certain position in the genome, which is mainly caused by the conversion or transversion of a single base; SNPs with conversion mutations account for about 2 / 3, and other SNPs are in similar level. The cytosine of the CpG dinucleotide is the most mutated site in the genome, most of which are methylated and can be spontaneously deaminated to form thymine. [0003] Va...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈宏王璟蓝贤勇淮永涛马亮赖新生胡沈荣雷初朝
Owner NORTHWEST A & F UNIV
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