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Low molecular weight glucan and preparation method and application thereof

A low-molecular-weight, dextran technology, applied in food preparation, medical preparations containing active ingredients, applications, etc., can solve problems such as being in the blank

Inactive Publication Date: 2010-03-24
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on astragalus polysaccharides mainly focuses on polysaccharides with larger molecular weight (Li Pengfei, Du Haiyan, Zhao Mengchun. Chemical and immunological research on astragalus polysaccharides. Shanghai Animal Husbandry and Veterinary Communication, 2005, (5): 4-6; Shan Junjie , Wang Shunchun, Liu Di, Hu Zhibi. Advances in chemical and pharmacological research on astragalus polysaccharides. Journal of Shanghai University of Traditional Chinese Medicine, 2000, 14(3): 61-65), while the research on low molecular weight polysaccharides is almost blank

Method used

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  • Low molecular weight glucan and preparation method and application thereof
  • Low molecular weight glucan and preparation method and application thereof
  • Low molecular weight glucan and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Separation and purification of astragalus polysaccharide

[0026] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the method described in the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5], the yield was 8.37%, and the purity was 85.74%.

[0027] (2) 50 g of MAPS obtained in (1) was taken, dissolved in an appropriate amount of distilled water, centrifuged at 5000 rpm for 20 min, the supernatant was dialyzed against water (molecular weight cut-off 3500), and the solution in the dialysis bag was concentrated to obtain MAPS-1 (12.6 g).

[0028] (3) Take MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and layer the supernatant with DEAE-Cellulose DE52 column (OH-type, 40×4.0cm, packing height 30cm) Preliminary separation was performed. First, distilled water was used as the eluent, and t...

Embodiment 2

[0030] Example 2: Separation and purification of astragalus polysaccharides

[0031] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the method described in the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5], the yield was 8.37%, and the purity was 85.74%.

[0032] (2) 50 g of MAPS obtained in (1) was taken, dissolved in an appropriate amount of distilled water, centrifuged at 5000 rpm for 20 min, the supernatant was dialyzed against water (molecular weight cut-off 3500), and the solution in the dialysis bag was concentrated to obtain MAPS-1 (12.6 g).

[0033] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and layer the supernatant with DEAE-Cellulose DE52 column (OH-type, 40×4.0cm, packing height 30cm) Preliminary separation was performed. First, distilled water was used as the eluent, ...

Embodiment 3

[0035] Example 3: Separation and purification of astragalus polysaccharide

[0036] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the method described in the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5], the yield was 8.37%, and the purity was 85.74%.

[0037] (2) 50 g of MAPS obtained in (1) was taken, dissolved in an appropriate amount of distilled water, centrifuged at 5000 rpm for 20 min, the supernatant was dialyzed against water (molecular weight cut-off 3500), and the solution in the dialysis bag was concentrated to obtain MAPS-1 (12.6 g).

[0038] (3) Take MAPS-14.0g obtained in (2), divide into 5 times, add 10ml of distilled water to dissolve, centrifuge, and carry out preliminary separation of supernatant by DEAE Sepharose F.F. column (40×4.0cm, filler height 30cm) chromatography. First, distilled water was used as the eluent, and then 0.01mol / ...

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Abstract

The invention discloses low molecular weight glucan which is characterized by being separated from radix astragalus. The invention also discloses a separation and purification method of the low molecular weight glucan and application of the low molecular weight glucan in preparing immunopotentiating drugs and adjunctive therapy drugs or health-care food.

Description

technical field [0001] The present invention relates to a low molecular weight glucan, a preparation method of the low molecular weight glucan and its use. Background technique [0002] Astragalus (Radix Astragalus) is the dried root of Astragalus membranaceus or Astragalus mongolica of the leguminous plant. Astragalus polysaccharide is one of its main active ingredients. Modern pharmacological studies have shown that astragalus polysaccharides have the functions of improving human immune function, anti-tumor, anti-radiation damage, regulating blood sugar, and anti-aging (Zhang Xiaomei. Immunomodulatory and anti-tumor effects of astragalus polysaccharides). Research Progress. Journal of Dalian University, 2003, 24(6): 101-104; Bao Wenqi, Lu Mei, Wang Zhixiang. Pharmacological Research Progress of Astragalus Polysaccharides. Henan Agricultural Science, 2005, (4): 78-80). [0003] At present, the research on Astragalus polysaccharides mainly focuses on the polysaccharides wit...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/716A61P35/00A61P37/04A23L1/30A23L1/28A23L1/09A23L29/30
Inventor 杨义芳林梦感
Owner SHANGHAI INST OF PHARMA IND
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