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A kind of rhizoma root polysaccharide effective part and preparation method thereof

A technology of effective parts and bean, which is applied to the effective parts of bean root polysaccharide and the field of preparation thereof, and can solve the problems of no acute toxicity and hepatotoxic medicinal value and the like.

Active Publication Date: 2021-05-04
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no research reports on the acute toxicity and liver toxicity of the active part of bean root polysaccharide and the research report on the medicinal value of the part after the toxicity is reduced.

Method used

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  • A kind of rhizoma root polysaccharide effective part and preparation method thereof
  • A kind of rhizoma root polysaccharide effective part and preparation method thereof
  • A kind of rhizoma root polysaccharide effective part and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of effective fractions of polysaccharides from bean root

[0023] a) Take 8 kg of mountain bean root decoction pieces, soak in 48 kg of water for 60 minutes, then heat and reflux for 1 hour, filter the decoction at 200 mesh, add 48 kg of water to heat and reflux and decoct for 1 hour, filter the decoction with 200 mesh, combine the two second filtrate to obtain the decoction of the root of the mountain bean;

[0024] b) At 75-80°C, decompress and concentrate the decoction of the root of the mountain bean root to a concentration of 1.5kg / L, then heat it under normal pressure at 98°C for 1.5 hours, and then cool it to room temperature to obtain the root of the mountain root water extract;

[0025] c) adding ethanol with a volume fraction of 95% to the aqueous extract of the bean root obtained in step b), stirring while adding, until the final volume fraction of ethanol is 80%, standing still, and collecting the precipitate;

[0026] d) Carry out g...

Embodiment 2

[0029] Embodiment 2: the stability investigation of preparation process

[0030] According to the preparation process of Example 1, three batches of effective fractions of the polysaccharides of bean root were repeatedly prepared, and the fingerprints of traditional Chinese medicine were prepared by HPLC, and the similarity between different batches of samples was compared. The HPLC conditions are as follows:

[0031]

[0032] See the experimental results figure 1 As shown, R in the figure is the sample of Example 1, and S1, S2, and S3 are respectively the batch 1-3 samples prepared in this embodiment, by figure 1 It can be seen that the similarity of fingerprints of samples R, S1, S2, and S3 reaches 99%, indicating that the preparation process of the present invention is stable and has good repeatability, and is expected to be industrialized and mass-produced.

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Abstract

The invention discloses an effective fraction of polysaccharides from the root of the bean root and a preparation method thereof. The effective fraction of the polysaccharide from the root of the bean root is obtained by carrying out alcohol precipitation on the water extract of the root of the bean root, followed by fractional drying, and contains a mass percentage of the total polysaccharide ≥65%, the mass percentage containing matrine <0.5%. Experiments show that the acute toxicity and hepatotoxicity of the active part of the polysaccharide of the root of the mountain bean provided by the present invention are significantly reduced, and it has a significant immune enhancement effect, which is of great significance for the research and development of the root of the mountain root with clinical application value; Moreover, the preparation method is simple, has good repeatability and high yield, is suitable for large-scale production, and has industrial application value.

Description

technical field [0001] The present invention relates to a kind of active part of bean root polysaccharide and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Shandougen is the dry root and rhizome of Sophora tonkinensis Gagnep, a leguminous plant, mainly produced in Guangxi, Guangdong, Guizhou and other provinces. It is bitter and cold in nature and belongs to the lung meridian. The Pharmacopoeia records that mountain bean root has the effects of clearing away heat and detoxification, benefiting the throat, and dispelling swelling and pain. The main components of mountain bean root are: alkaloids, flavonoids, organic acids, saponins and polysaccharides. Pharmacological experiments show that Shandou root has various pharmacological activities such as anti-tumor, anti-virus, anti-hepatitis, and anti-arrhythmia, and according to research reports, most of the alkaloids contained in it are the material basis of these phar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/489A61P37/04
CPCA61K36/489A61K2236/331A61K2236/51A61K2236/53
Inventor 邓中平陈江华陈龙李春启杨以阜邵珍陈春麟
Owner SHANGHAI UNIV OF T C M
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