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Low molecular weight glucan and preparation method and application thereof

A low-molecular-weight, glucan technology, used in anti-tumor drugs, drug combinations, allergic diseases, etc., can solve problems such as blanks

Inactive Publication Date: 2010-03-24
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on astragalus polysaccharides mainly focuses on polysaccharides with larger molecular weight (Li Pengfei, Du Haiyan, Zhao Mengchun. Chemical and immunological research on astragalus polysaccharides. Shanghai Animal Husbandry and Veterinary Communication, 2005, (5): 4-6; Shan Junjie , Wang Shunchun, Liu Di, Hu Zhibi. Advances in chemical and pharmacological research on astragalus polysaccharides. Journal of Shanghai University of Traditional Chinese Medicine, 2000, 14(3): 61-65), while the research on low molecular weight polysaccharides is almost blank

Method used

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  • Low molecular weight glucan and preparation method and application thereof
  • Low molecular weight glucan and preparation method and application thereof
  • Low molecular weight glucan and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Separation and purification of astragalus polysaccharide

[0026] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the method described in the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5]. The yield was 8.37%, and the purity was 85.74%.

[0027] (2) Take 50 g of MAPS obtained in (1), dissolve it in an appropriate amount of distilled water, centrifuge at 5000 rpm for 20 min, dialyze the supernatant against water (molecular weight cut-off 3500), and concentrate the solution in the dialysis bag to obtain MAPS-1 (12.6 g).

[0028] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and use the DEAE-Cellulose DE52 column (OH-type, 40×4.0cm, packing height 30cm) layer on the supernatant Analysis for preliminary separation. First use distilled water as the eluent, then elute with ...

Embodiment 2

[0030] Example 2: Separation and purification of astragalus polysaccharide

[0031] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the method described in the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5]. The yield was 8.37%, and the purity was 85.74%.

[0032] (2) Take 50 g of MAPS obtained in (1), dissolve it in an appropriate amount of distilled water, centrifuge at 5000 rpm for 20 min, dialyze the supernatant against water (molecular weight cut-off 3500), and concentrate the solution in the dialysis bag to obtain MAPS-1 (12.6 g).

[0033] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and use the DEAE-Cellulose DE52 column (OH-type, 40×4.0cm, packing height 30cm) layer on the supernatant Analysis for preliminary separation. First use distilled water as the eluent, then elute with ...

Embodiment 3

[0035] Example 3: Separation and purification of astragalus polysaccharide

[0036] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the method described in the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5]. The yield was 8.37%, and the purity was 85.74%.

[0037] (2) Take 50 g of MAPS obtained in (1), dissolve it in an appropriate amount of distilled water, centrifuge at 5000 rpm for 20 min, dialyze the supernatant against water (molecular weight cut-off 3500), and concentrate the solution in the dialysis bag to obtain MAPS-1 (12.6 g).

[0038] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and use the DEAE Sepharose F.F. First use distilled water as the eluent, then elute with 0.01mol / L NaCl solution, the automatic fraction collector collects each fraction separately, the differential i...

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Abstract

The invention discloses low molecular weight glucan which is characterized by being separated from radix astragalus. The invention also discloses a separation and purification method of the low molecular weight glucan and application of the low molecular weight glucan in preparing immunopotentiating drugs and adjunctive therapy drugs or health-care food.

Description

technical field [0001] The invention relates to a low-molecular-weight dextran, a preparation method of the low-molecular-weight dextran and an application thereof. Background technique [0002] Radix Astragalus (Radix Astragalus) is the dried root of Astragalus membranaceus or Astragalus mongolica of the leguminous plant. It is sweet in taste and warm in nature. It returns to the lung and spleen meridian. Astragalus polysaccharide is one of its main active ingredients. Modern pharmacological studies have proved that astragalus polysaccharide has the effects of improving human immune function, anti-tumor, anti-radiation damage, regulating blood sugar, and anti-aging (Zhang Xiaomei. Immunomodulatory and anti-tumor effects of astragalus polysaccharides Research progress. Journal of Dalian University, 2003, 24(6): 101-104; Bao Wenqi, Lu Mei, Wang Zhixiang. The pharmacological research progress of astragalus polysaccharides. Henan Agricultural Sciences, 2005, (4): 78-80). [00...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61P37/04A61P35/00
Inventor 杨义芳林梦感
Owner SHANGHAI INST OF PHARMA IND
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