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Low-molecular-weight glucan, its preparation method and use

A low molecular weight, glucan technology, applied in food preparation, medical preparations containing active ingredients, applications, etc., can solve problems such as blank

Inactive Publication Date: 2012-07-04
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on astragalus polysaccharides mainly focuses on polysaccharides with larger molecular weight (Li Pengfei, Du Haiyan, Zhao Mengchun. Chemical and immunological research on astragalus polysaccharides. Shanghai Animal Husbandry and Veterinary Communication, 2005, (5): 4-6; Shan Junjie , Wang Shunchun, Liu Di, Hu Zhibi. Advances in chemical and pharmacological research on astragalus polysaccharides. Journal of Shanghai University of Traditional Chinese Medicine, 2000, 14(3): 61-65), while the research on low molecular weight polysaccharides is almost blank

Method used

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  • Low-molecular-weight glucan, its preparation method and use
  • Low-molecular-weight glucan, its preparation method and use
  • Low-molecular-weight glucan, its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Separation and purification of astragalus polysaccharides

[0025] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5]. The yield was 8.37% and the purity was 85.74%.

[0026] (2) Take 50 g of the MAPS obtained in (1), dissolve in an appropriate amount of distilled water, centrifuge at 5000 rpm for 20 min, and dialyze the supernatant against water (molecular weight cut-off 3500), and concentrate the solution in the dialysis bag to obtain MAPS-1 (12.6 g).

[0027] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and use DEAE-Cellulose DE52 column (OH-type, 40×4.0cm, packing height 30cm) for the supernatant. Analyze for preliminary separation. First use distilled water as the eluent, and then use 0.01mol / L and 0.1mol / L NaCl solution t...

Embodiment 2

[0029] Example 2: Separation and Purification of Astragalus Polysaccharides

[0030] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5]. The yield was 8.37% and the purity was 85.74%.

[0031] (2) Take 50 g of the MAPS obtained in (1), dissolve in an appropriate amount of distilled water, centrifuge at 5000 rpm for 20 min, and dialyze the supernatant against water (molecular weight cut-off 3500), and concentrate the solution in the dialysis bag to obtain MAPS-1 (12.6 g).

[0032] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, and use DEAE-Cellulose DE52 column (OH-type, 40×4.0cm, packing height 30cm) for the supernatant. Analyze for preliminary separation. First use distilled water as the eluent, and then use 0.01mol / L and 0.1mol / L NaCl solution t...

Embodiment 3

[0034] Example 3: Separation and Purification of Astragalus Polysaccharides

[0035] (1) Astragalus polysaccharide (MAPS) was separated and extracted according to the patent [Yang Yifang, Xu Haiyan, Huang Chunyue. A method for refining astragalus polysaccharide. Chinese invention patent: 200610024178.5]. The yield was 8.37% and the purity was 85.74%.

[0036] (2) Take 50 g of the MAPS obtained in (1), dissolve in an appropriate amount of distilled water, centrifuge at 5000 rpm for 20 minutes, dialyze the supernatant against water (molecular weight cut-off 3500), and concentrate the solution in the dialysis bag to obtain MAPS-1 (12.6 g).

[0037] (3) Take the MAPS-14.0g obtained in (2), divide it into 5 times, add 10ml of distilled water to dissolve, centrifuge, the supernatant is separated by DEAE Sepharose F.F. column (40×4.0cm, filler 30cm high) chromatography. First, distilled water is used as the eluent, and then eluted with 0.01mol / L NaCl solution. The automatic partial collecto...

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PUM

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Abstract

The invention discloses a low-molecular-weight glucan, characterized in that the glucan is obtained by separating from radix astragali. The invention also discloses a method for separating and purifying the low-molecular-weight glucan. In addition, the invention discloses a use of the low-molecular-weight glucan in the preparation of a medicament for the immunopotentiation and the adjutant therapy of tumor or a health food.

Description

Technical field [0001] The invention relates to a low-molecular-weight glucan, a preparation method and use of the low-molecular-weight glucan. Background technique [0002] Radix Astragalus (Radix Astragalus) is the dried root of legume Astragalus membranaceus or Astragalus mongolicus. It is sweet in taste and warm in nature. It belongs to the lung and spleen meridian. It is an important Chinese medicine for replenishing qi. Astragalus polysaccharide is one of its main effective ingredients. Modern pharmacological studies have proved that Astragalus polysaccharide has the functions of improving human immune function, anti-tumor, anti-radiation damage, regulating blood sugar, anti-aging, etc. (Zhang Xiaomei. Immunomodulatory and anti-tumor effects of Astragalus polysaccharide Research progress. Journal of Dalian University, 2003, 24(6): 101-104; Bao Wenqi, Lv Mei, Wang Zhixiang. Pharmacological research progress of astragalus polysaccharides. Henan Agricultural Sciences, 2005, (4...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A61K31/716A61P35/00A61P37/04A23L1/30A23L1/28A23L1/09A23L29/30A23L33/00
Inventor 杨义芳林梦感
Owner SHANGHAI INST OF PHARMA IND CO LTD
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