Transgenosis construct and application thereof in preparing space-time adjustable liver damage model

Abstract

The invention relates to a transgenosis construct and an application thereof in preparing a space-time adjustable liver damage model and discloses a construct combination used for preparing the space-time adjustable liver damage model. The construct combination comprises a construct expressing space-time adjustable liver damage induction induced gene and a construct expressing Cre recombinant enzyme. The invention also discloses cells containing the construct and a method for preparing the space-time adjustable liver damage model. The obtained animal model can efficiently induce the liver damage at any time, and provides an effective way for the research of hepatic cell transplantation and liver replantation.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a transgene construct and its application in preparing a space-time adjustable liver injury model. Background technique

[0002] Liver organ transplantation is currently the main means of clinical treatment of acute liver failure, end-stage liver disease and metabolic liver disease. However, due to the shortage of donor livers, the clinical application of liver transplantation has been severely limited. About 14,000 people in the United States wait for liver transplantation every year, 50% of them wait for more than one year, and about 1,300 people die each year because they cannot wait for liver transplantation. The waiting list for organ transplants in my country is also increasing. Therefore, there is an urgent need for other methods to improve liver function and prolong the survival time of patients to wait for liver transplantation, or to replace liver transplantati...

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