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Composition for lipolysis using surface-modified gas-generating nanoparticles

A lipolysis and surface modification technology, which is applied in drug combination, drug delivery, cosmetic preparations, etc., can solve side effects and other problems, and achieve the effect of improving or treating obesity and minimizing side effects

Pending Publication Date: 2022-07-29
超新星生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, conventional lipolytic agents have the problem of inducing various side effects by affecting nerve cells, muscle cells, and bone cells that contain fat components around the fat cells when they are locally injected into fat cells.

Method used

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  • Composition for lipolysis using surface-modified gas-generating nanoparticles
  • Composition for lipolysis using surface-modified gas-generating nanoparticles
  • Composition for lipolysis using surface-modified gas-generating nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1: Preparation of polylactide-glycolide (PLGA, poly) with surface modification by optimal peptides (P1-P7) (lactide-co-glycolide) gas-generating nanoparticles

[0097] PLGA gas-generating nanoparticles were prepared according to the method disclosed in Example 2 of Korean Patent Publication No. 10-1613606. Surface modification was performed by subjecting the prepared PLGA gas-generating nanoparticles to carbodiimide-bonding reaction with optimal peptides (P1-P7).

[0098] Specifically, PLGA gas-generating nanoparticles (0.5 mM) were suspended in 0.1 M MES buffer (pH 7.0), and EDC (0.5 mM) / sulfo- NHS (0.5mM). The activated PLGA gas-generating nanoparticles were reacted with ethylenediamine (0.5 mM) for one day, washed three times with deionized water, and then resuspended in MES buffer (pH 7.0). To the solution in which the functionalized PLGA gas-generating nanoparticles were suspended, EDC / sulfo-NHS and the optimal peptide described in Table 1 below (the ...

Embodiment 2

[0101] Example 2: 3T3-L1 cell culture and differentiation

[0102] 3T3-L1 undifferentiated cells were cultured in glass bottom cell culture dishes (confocal dishes) or 96-well plates in Dulbecco's modified Eagle's medium (Dulbecco) containing 10% fetal calf serum (BCS, Bovine Calf Serum). 'sModified Eagle's Medium, DMEM) at 37°C, 5% CO 2 conditioned culture. After 3T3-L1 cells were confluenced (confluence), they were grown to day 2 (Day 0), in a growth medium containing 10% fetal bovine serum, using MDI (0.5 μM methylisobutylxanthine, 1 μM dexamethasone and 10 mg / L insulin) were treated for 2 days. Next, after an additional 2 days of treatment in growth medium containing only 10 mg / L of insulin, the growth medium was replaced every 2 days and cultured, and all experiments were started on day 7 after differentiation induction.

Embodiment 3

[0103] Example 3: Analysis of 3T3- of PLGA nanoparticles (PNP) surface-modified by optimal peptides (P1-P5, P7) Intracellular engraftment in L1 cells

[0104] To analyze the 3T3-L1 intracellular engraftment of PLGA nanoparticles surface-modified by the optimal peptides, to the optimal peptides [P1-P5, P7 (in this case, the combination of P1 and P3; molar ratio = 1:1) ] Fluorescent substances were introduced into the surface of the surface-modified PLGA nanoparticles.

[0105] Specifically, the PLGA nanoparticles into which the fluorescent substance (Cy5) was introduced and surface-modified by the optimal peptide [P1-P5, P7 (at this time, the combination of P1 and P3; molar ratio=1:1)] were prepared at 0.5 mg 3T3-L1 cells were treated at a concentration of / ml for 6 hours, and LysoTracker Green DND-26, which stains lysosomes, was added to confirm the engraftment into 3T3-L1 cells. The medium was removed and washed three times with phosphate buffered saline (PBS), fixed wit...

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Abstract

The present invention relates to a composition for lipolysis, comprising: a biocompatible polymer surface-modified by an adipocyte-targeting peptide or a cell-penetrating peptide; and a surface-modified gas-generating nanoparticle comprising a fine calcium carbonate (Fine-Grain CalcimumCarbonate) crystal, which is encapsulated in the inside of the biocompatible polymer. The present invention also relates to a method for preparing the surface-modified gas-generating nanoparticle, and a method for preparing the surface-modified gas-generating nanoparticle.

Description

technical field [0001] The present invention relates to a composition for lipolysis using gas-generating nanoparticles surface-modified with an optimal peptide. Background technique [0002] Due to the huge demand in the body sculpting market, which continues to grow every year, various methods of liposuction and lipolysis have been developed. Considering the shortcomings of previous liposuction, in the field of weight regulation and body sculpting, manufacturers have shifted their main interest from traditional liposuction to developing minimally invasive and even non-invasive methods of lipolysis. [0003] The non-invasive lipolysis method is a method of dissolving the cellulite by injecting a drug or gas into the part where fat is lost slowly through weight loss or exercise, or in the part where cellulite (denatured fat) is formed under the skin and the effect of fat reduction is not achieved, and induces the formation of adipose tissue. Contraction or circulation of lym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K9/00A61K33/10A61P3/04A61K8/02A61K8/64A61K8/72A61K8/19A61Q19/06
CPCC07K17/14A61P3/04A61K9/5153A61K9/0019A61K38/00A61K33/10A61K2800/57A61K2800/91A61K8/85A61K2800/413A61K8/64A61K8/19A61K2800/22A61Q19/06A61K8/0241A61K47/6937A61K47/64A61K2300/00A61K9/5169A61K9/513A61K9/0014C07K7/06C07K7/08A61K8/0245A61K8/72A61K47/62A61K47/6923A61K9/0004
Inventor 郑恩周徐艺让
Owner 超新星生物有限公司
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