Method and device for detecting organic phosphorus pesticide

Abstract

The invention relates to detection of organic phosphorus pesticide, in particular to a method and a device for detecting organic phosphorus pesticide. The method comprises the following concrete steps: inserting an ion selective electrode into a measuring pool and adopting seeped inner filling liquid during electrode detection as a substrate to generate a contrast signal; inserting the electrode into the measuring pool containing butyrylcholine esterase and standard pesticide and adopting the seeped inner filling liquid during electrode detection as the substrate to generate a standard signal; obtaining a standard curve by a potential difference of the standard signal and the contrast signal; finally inserting the electrode into the measuring pool containing butyrylcholine esterase and a sample to be detected and adopting the seeped inner filling liquid during electrode detection as the substrate to generate a sample signal; and obtaining the concentration of the organic phosphorus pesticide to be detected by the potential difference. The device adopts the structure that butyrylcholine inner filling liquid is contained in the electrode, an internal reference ratio electrode is inserted into the butyrylcholine inner filling liquid, and the bottom of the electrode is stuck with a polymer sensitive film. The electrode inner filling liquid can continuously seep, the enzyme substrate is generated at the original site of the surface of the electrode without manually adding the substrate, the operation is simple and convenient, and an error is prevented.

Description

technical field

[0001] The invention relates to the detection of organophosphorus pesticides, in particular to a method for detecting organophosphorus pesticides and a device thereof. Background technique

[0002] According to the chemical characteristics and toxicological properties of organophosphorus pesticides, there are three main analytical methods for the detection of organophosphorus pesticides, namely spectroscopy, chromatography and enzyme inhibition. The spectroscopic method requires a special chromogenic reagent, and the sensitivity is not high. There are many interference factors in the test, and false negatives are prone to occur. Chromatography has defects such as slow monitoring speed, poor continuity, high analysis cost, secondary pollution and inability to monitor online. The biggest advantage of the enzyme inhibition method is that it is easy to operate, fast, and does not require expensive instruments. It is especially suitable for on-site detection and ...

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