Real-time fluorescence quantitative PCR excitation detection system

A fluorescence excitation and light source technology, applied in the field of fluorescence quantitative PCR detection system, can solve the problems of difficult grating fabrication, low utilization rate, affecting detection accuracy, etc., and achieves easy maintenance and operation, fast tuning speed, and good monochromaticity. Effect

Inactive Publication Date: 2010-04-28
XIAN TIANLONG SCI & TECH
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  • Abstract
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Problems solved by technology

However, this method has major defects such as difficult grating production, side lobes affecting detection accuracy, and low utilization rate.

Method used

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  • Real-time fluorescence quantitative PCR excitation detection system
  • Real-time fluorescence quantitative PCR excitation detection system

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Embodiment Construction

[0026] Referring to the figure, a quantitative PCR system based on a tunable filter to realize full-wavelength fluorescence excitation and detection of the present invention includes:

[0027] A fluorescence excitation module composed of a light source 1, a light collecting device 2 and an adjustable filter I (marker 3);

[0028] A fluorescence detection module composed of an adjustable filter II (label 5), a photodetector 7 and a photoelectric conversion device 8;

[0029] A control unit composed of a wavelength tuning device 9 and a main control computer 10; wherein the wavelength tuning device 9 is used to adjust the filtering wavelength of the tunable filter I and the tunable filter II;

[0030] A heat cycle system 11 consisting of a reaction test tube 12, a metal temperature block 14 and a constant temperature heat cover 13;

[0031] The thermal cycle system 11 communicates with the first tunable filter 3 through the incident optical fiber 4, and communicates with the se...

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Abstract

The invention provides a quantitative PCR detection system which is based on a tunable filter and can realize the full wavelength fluorescence excitation detection, and the quantitative PCR detection system comprises a fluorescence excitation module consisting of a light source, a light gathering device and a first adjustable filter, a fluorescence detection module consisting of a second adjustable filter, an optical detector and a photoelectric conversion device, a control unit consisting of a wavelength tuning device and a main control computer, and a heat circulation system consisting of a reaction test tube, a metal warm block and a constant temperature hot cover. The light gathering is carried out on excited light, then the wavelength section is carried out by the tunable filter, the excited fluorescence passes through the tunable filter, the components of the excited light and other interference signals are filtered, and then the optical detector and the photoelectric conversion device are used for delivering to the main control computer for carrying out data analysis.

Description

technical field [0001] The invention relates to a fluorescence quantitative PCR detection system, in particular to a quantitative PCR detection system based on a tunable filter to realize full-wavelength fluorescence excitation and detection (tunable acousto-optic and liquid crystal). Background technique [0002] The working principle of the real-time fluorescent quantitative PCR detection system is to make the reactants undergo temperature cycling between specific denaturation temperature, annealing temperature and extension temperature, and amplify the target DNA by millions of times; at the same time, by using different wavelengths of excitation light (Multi-excitation) or single-wavelength excitation light (single excitation) irradiates the test tube. When the reagent in the test tube is excited to fluoresce, the fluorescence intensity signal collected by the optical sensor is sent to the computer for real-time data analysis. [0003] The current mainstream real-time fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12M1/34C12Q1/68G02F1/01
Inventor 李明苗保刚臧留琴李壮刘慧仙梁光勤
Owner XIAN TIANLONG SCI & TECH
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