Direct contact type cell co-culture device

A contact and co-cultivation technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of inability to achieve direct contact co-culture of cells, and achieve reliable results and simple operation Effect

Inactive Publication Date: 2010-05-05
SHANDONG UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently commonly used cell co-culture devices need to use expensive Matrix gel (Matrix gel) and Transwell culture chambers in combination, which can only carry out indirect contact co-cultivat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Direct contact type cell co-culture device
  • Direct contact type cell co-culture device
  • Direct contact type cell co-culture device

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1:

[0021] A direct contact cell co-cultivation device, such as figure 1 with figure 2 As shown, it consists of culture well 2, culture plate 1 and upper cover 3. The culture well 2 is set on the culture plate 1, and the upper cover 3 covers the culture plate 1 and seals the culture well 2; the culture well 2 is provided with an isolation plate 9. Separate the culture well 2 into two culture chambers, which can realize the co-cultivation of two different adherent cells.

[0022] There are 1 to 48 culture holes 2 and barbs 7 are provided on the outer side wall of the upper part of the culture holes 2.

[0023] There is one isolation plate 9 in the 2 inside the cultivation hole.

[0024] The two vertical edges of the isolation plate 9 are installed in the side fixing grooves 10 provided in the culture hole, the bottom edge of the isolation plate 9 is pressed on the elastic pad 11 on the bottom surface 12 of the culture hole, and the upper edge of the isolation plate 9 i...

Example Embodiment

[0026] Example 2:

[0027] A direct contact cell co-cultivation device, the structure is the same as that of Example 1, but the difference is: two parallel isolation plates 9 are provided in the culture well 2, and the culture well 2 is divided into three culture chambers, which can achieve three Co-culture of different kinds of adherent cells, such as image 3 Shown.

Example Embodiment

[0028] Example 3:

[0029] A direct contact cell co-cultivation device, the structure is the same as that of Example 1, but the difference is: three parallel isolation plates 9 are provided in the culture well 2, and the culture well 2 is divided into four culture cells, which can realize four Co-culture of different kinds of adherent cells, such as Figure 4 Shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a direct contact type cell co-culture device which belongs to the technical field of cell co-culture devices. The direct contact type cell co-culture device comprises a culture hole, a culture plate and an upper cover, wherein the culture hole is arranged on the culture plate, and the upper cover is covered on the culture plate and seals the culture hole; an isolation plate is arranged in the culture hole, and the culture hole is divided into a plurality of small culture chambers. A reverse hook is arranged on the outer side wall of the upper part of the culture hole, two upright edges of the isolation plate are installed in a side fixing groove arranged in the culture hole, the bottom edge of the isolation plate is provided with an elastic pad, and the elastic pad is pressed on the bottom surface of the culture hole; when the culture hole is not pressed, the height of the isolation plate is slightly higher than the depth of the culture hole, and the upper edge of the isolation plate is installed in an upper fixing groove; and the end of the upper fixing groove is provided with a connecting line with a circular ring. Before being unsealed, the absolutely aseptic state of the cell culture device can be ensured. The invention has the advantages of simple operation and reliable effect, can clearly observe and contrast the situation of the growth change of cells at the edges of the small culture chambers, can realize the co-culture of various cells and does not influence the normal adherence and multiplication of the cells.

Description

technical field [0001] The invention relates to a direct contact cell co-culture device, which belongs to the technical field of cell co-culture devices. Background of the invention: [0002] Putting different cells in the same environment for co-cultivation, through the study of the interaction and transformation between cells, is more and more used in the field of medical research. Cell co-culture is mainly divided into direct contact co-culture and non-direct contact co-culture. However, the currently commonly used cell co-culture devices need to use expensive Matrix gel (Matrix gel) and Transwell culture chambers in combination, which can only carry out indirect contact co-cultivation between two types of cells, and cannot achieve direct cell contact co-cultivation. Due to the current requirements of cell co-culture research, it is urgent to develop a direct contact cell co-culture device. Invention content: [0003] Aiming at the shortcomings of existing cell co-cul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00
Inventor 周长扩徐祗顺陈军
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap