Pseudomonas fluorescens CKD18 and application thereof

A technology of Pseudomonas fluorescens and bacteria agent, applied in the direction of application, bacteria, biochemical equipment and methods, etc., can solve the problems that Pseudomonas fluorescens application has not been found, and achieve high absorption effect

Inactive Publication Date: 2010-05-19
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Pseudomonas fluorescens CKD18 and application thereof
  • Pseudomonas fluorescens CKD18 and application thereof
  • Pseudomonas fluorescens CKD18 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Identification of embodiment 1 CKD18

[0029] The CKD18 strain was identified as Pseudomonas fluorescens based on its morphological characteristics, physiological and biochemical characteristics, and 16S rDNA sequence determination results. The specific identification results are as follows:

[0030] 1. Physiological and biochemical characteristics

[0031] The physiological and biochemical characteristics of Pseudomonas fluorescens CKD18 are shown in Table 1: Gram-negative bacteria, strictly aerobic, gelatin liquefaction, nitrate reduction, urease, citrate utilization, arginine dihydrolase, lecithinase, Oxidase and H2S production are negative, starch hydrolysis, denitrification, lipase, and VP are all positive; fructose can be used as a carbon source, but sucrose, tartaric acid, glycine, etc. cannot be used as a carbon source.

[0032] Table 1 CKD18 physiological and biochemical characteristics determination items

[0033]

[0034] +, positive reaction; -, negati...

Embodiment 2

[0038] Embodiment 2 CKD18 bacterial strain plate phosphorus dissolving effect detection

[0039] Pick a single bacterial colony of CKD18 with a sterile toothpick, streak and purify it, and inoculate it into Pikovsaia’s Medium (PKO) solid medium (glucose 10.0 g, yeast extract 0.50 g, Ca 3 (PO 4 )25.0g, (NH 4)2 SO 4 0.5g, KCl 0.2g, MgCl 2 0.1g, MnSO 4 0.1mg, FeSO 4 0.1mg, agar 12.0g, H 2 (O 1000ml, pH7.0-7.2, sterilized at 121°C for 30min) plate, cultured upside down in a constant temperature incubator at 28°C for 7 days, the diameter of the phosphorus-dissolving circle of CKD18 was determined to be 16mm, and the phosphorus-dissolving ability remained stable. (See image 3 )

Embodiment 3

[0040] Phosphorus-dissolving effect detection in embodiment 3CKD18 bacterial strain solution

[0041] The CKD18 strain was inoculated into LB liquid culture medium, cultured in a shaker at 170r / min at 28°C for 48 hours, and 50 μl of the bacterial solution was inoculated into PKO liquid medium, and cultured in a shaker at 28°C at 170r / min for 10 days. Centrifuge the culture solution at 12000r / min at 4°C for 5min, take 100μL of the supernatant (containing 5-25μg of phosphorus), put it into a 50ml volumetric flask, dilute it with water to about 30ml, add 2 drops of dinitrophenol indicator, dropwise 4mol / LNaOH until the solution just turns yellow, then add 2mol / L (1 / 2H 2 SO 4 ) 1 drop, so that the yellow color of the solution has just receded. Add 5.00ml molybdenum antimony anti-color agent, dilute to volume with water, and shake well. After standing at a room temperature higher than 15°C for 30 minutes, measure the absorbance at a wavelength of 882nm with a cuvette with a 1cm ...

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Abstract

The invention provides Pseudomonas fluorescens CKD18, which has a strain preservation number of CGMCC No.3227. Experiments show that the strain has efficient stable phosphate-dissolving capability and can promote the P, N and K absorption of plants so as to promote plant growth. The strain can be made into various conditioners or bio-fertilizers and is highly worthy to be applied.

Description

technical field [0001] The invention relates to a new microbial strain and its application, in particular to a strain of Pseudomonas fluorescens and its application in activating insoluble phosphorus in soil and promoting the absorption and growth of P, N and K elements by plants. Background technique [0002] Phosphorus is one of the main nutrients needed by plants. The amount of total phosphorus in my country's soil is considerable, but more than 95% of phosphorus exists in ineffective forms such as stable aluminosilicate and apatite, which are difficult for plants to use directly, and the content of available phosphorus that can be absorbed and utilized by plants is low. Therefore, most crops and trees must be topdressed with phosphorus fertilizer. However, the seasonal utilization rate of the applied phosphorus fertilizer is only 10%-25%, and at least 70%-90% of phosphorus enters the soil and becomes a fixed form that is difficult to be absorbed and utilized by crops. A...

Claims

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Application Information

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IPC IPC(8): C09K17/00C05F11/08C12N1/20C12R1/39C09K101/00
Inventor 郭岩彬吴文良李军慧夏娜袁超磊牟成香王开勇周华宁李金云
Owner CHINA AGRI UNIV
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