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Pholiota adiosapose polysaccharide and preparation method thereof

A technology of yellow umbrella and polysaccharide, which is applied to the preparation field of the polysaccharide, can solve the problems of low yield, long production cycle, unstable product quality, etc., and achieves the advantages of improving biological activity, removing free radicals in the body, and improving human immunity. Effect

Inactive Publication Date: 2012-06-20
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production cycle of the fruiting body of the umbrella is long, the yield is not high, the product quality is unstable, the extraction rate of the polysaccharide from the umbrella is low, and the purity is not high. At present, there are no reports on the purification and advanced structure of the polysaccharide extracted from the umbrella. , the physiological activity of the purified polysaccharide has not been reported

Method used

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  • Pholiota adiosapose polysaccharide and preparation method thereof
  • Pholiota adiosapose polysaccharide and preparation method thereof
  • Pholiota adiosapose polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Cultivation of the mycelium of the yellow mushroom

[0035] ① Liquid medium formula: glucose 20g / L, yeast extract powder 8g / L, vitamin B 1 300μg / L, Mg 2 SO 4 0.4g / L, KH 2 PO 4 1g / L, K 2 HPO 4 1g / L, pH 6, high temperature sterilization for use.

[0036]②Seed culture: According to the aseptic operation method, use a 0.5cm diameter puncher to get the mother seed, take the solid seed with an inoculum size of 3 pieces, and transfer it to the sterilized liquid medium (the inoculum size is about 3%). Cultivation temperature: 12 days at 25°C; the rotation speed of the shaker is 120r / min-140r / min.

[0037] ③Fermentation culture: inoculate the seed culture strains into the fermenter by aseptic operation method, the inoculum size is 8% (V / V), the fermentation medium is: glucose 30g / L, corn steep liquor 20g / L, dihydrogen phosphate Potassium 2g / L, pH value 6.5-7.0, control temperature at 25°C, stirrer speed at 150-180r / min, ventilation rate at 0.8 (V / V·min). ...

Embodiment 2

[0038] Example 2 Liquid culture of parasol mycelium

[0039] ① Liquid medium formula: glucose 15g / L, yeast extract powder 10g / L, vitamin B 1 200μg / L, Mg 2 SO 4 0.5g / L, KH 2 PO 4 0.8g / L, K 2 HPO 4 1.2g / L, pH 6, high temperature sterilization for use.

[0040] ②Seed culture: According to the aseptic operation method, use a 0.5cm diameter puncher to take the mother seed, take the solid seed with an inoculum size of 5 pieces, and transfer it to the sterilized liquid medium (the inoculum size is about 6%). Cultivation temperature: 10 days at 25°C; shaker rotation speed 120r / min-140r / min.

[0041] ③Fermentation culture: inoculate the seed culture strains into the fermenter by aseptic operation method, the inoculum size is 10% (V / V), the fermentation medium is: glucose 40g / L, corn steep liquor 25g / L, dihydrogen phosphate Potassium 2.5g / L, pH value 6.5, control temperature at 25°C, stirrer speed at 150-180r / min, ventilation rate at 1.5 (V / V·min). After the end of fermentat...

Embodiment 3

[0042] Example 3 Liquid culture of parasol mycelium

[0043] ① Liquid medium formula: glucose 25g / L, yeast extract powder 6g / L, vitamin B 1 400μg / L, Mg 2 SO 4 0.3g / L, KH 2 PO 4 0.1.2g / L, K 2 HPO 4 0.8g / L, pH 6, high temperature sterilization for use.

[0044] ②Seed culture: According to the aseptic operation method, use a 0.5cm diameter puncher to get the mother seed, take the solid bacterial seed with an inoculum size of 2 pieces, and transfer it to the sterilized liquid medium (the inoculum size is about 2%). Cultivation temperature: 16 days at 25°C; shaker rotation speed 120r / min-140r / min.

[0045] ③Fermentation culture: inoculate the seed culture strains into the fermenter by aseptic operation method, the inoculum size is 5% (V / V), the fermentation medium is: glucose 30g / L, corn steep liquor 15g / L, dihydrogen phosphate Potassium is 1.5g / L, pH value is 6.5, the control temperature is 25°C, the rotation speed of the stirrer is 150-180r / min, and the ventilation rat...

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Abstract

The invention provides pholiota adiosapose polysaccharide, which is extracted from a pholiota adiosapose mycelium or fruiting body and prepared by purification, is a heteropolysaccharide comprising four monosaccharides, namely glucose, fructose, mannose and galactose, can improve body immunity and has functions of preventing cancers, resisting cancers, resisting ageing and the like, and can be used in combination with ganoderma lucidum polysaccharide, coriolus versicolor polysaccharide and the like. The invention also provides a method for preparing the pholiota adiosapose polysaccharide. Themethod is an improvement on water extract method and makes the yield of the polysaccharide reach over 23 percent, the content of the polysaccharide reach 55 percent, and the growth amount of the mycelium after the crude polysaccharide is purified up to 93.67 percent. Combined with a mycelium liquid culture technique, the method can increase the growth amount of the mycelium by over 10 percent so as to decrease the production cost, improve the production efficiency and in particular greatly improve bioactivity of polysaccharide products.

Description

technical field [0001] The present invention relates to a polysaccharide of the umbrella, which is a polysaccharide extracted from the umbrella of the umbrella. The purified product of the polysaccharide can improve human immunity. The invention also relates to a preparation method of the polysaccharide. Background technique [0002] The scientific name of the yellow umbrella is Pholiota adiposa (Fr.) Qu él. In taxonomy, it belongs to Eumycota, Basidiomycotina, Hymenomycetes, Agaricales, Strophariaceae, and GenusPholipta. An edible and medicinal fungus. "China's Large Fungi" (Mao Xiaolan, 2000) (1) It is recorded that there is a layer of mucilage on the surface of the fruit body of Huangsan, and the polysaccharide can be extracted by salt water, warm water, alkaline solution or organic solvent, and the inhibition rate of this polysaccharide on mouse sarcoma 180 and Ehrlich ascites carcinoma reaches 80% to 90%. ". Su Yanyou (2004) (2) Using the orthogonal test method, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04C12N1/20A23L1/30A23L1/09C12R1/645A23L29/30A23L33/105A23L33/125
Inventor 胡清秀
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI