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Quick detection reagent for drug sensitivity of mycoplasma pneumoniae and preparation method

A technology of mycoplasma pneumoniae and detection reagents, applied in biochemical equipment and methods, methods based on microorganisms, measurement/inspection of microorganisms, etc., can solve problems such as unseen commodities and patent literature reports, and meet the criteria of intuitiveness and specificity Strong, stable performance

Inactive Publication Date: 2011-10-05
黑龙江省汇力医疗器械有限公司
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  • Application Information

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Problems solved by technology

Mycoplasma pneumoniae drug susceptibility detection reagents have not been reported in related products and patent literature so far

Method used

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  • Quick detection reagent for drug sensitivity of mycoplasma pneumoniae and preparation method
  • Quick detection reagent for drug sensitivity of mycoplasma pneumoniae and preparation method
  • Quick detection reagent for drug sensitivity of mycoplasma pneumoniae and preparation method

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Embodiment Construction

[0052] The present invention will be further described in detail below in conjunction with specific examples.

[0053] A rapid detection reagent for drug susceptibility of mycoplasma pneumoniae comprises a mycoplasma pneumoniae culture medium and a drug sensitivity plate, wherein the mycoplasma pneumoniae culture medium comprises the following components: basal culture medium, calf serum, yeast powder and glucose. Add trace amounts of phenol red and penicillin. See Table 1 for specific examples.

[0054] Table 1 Mycoplasma pneumoniae medium composition mass percentage%

[0055]

[0056] In Table 1, the basal medium includes the following components: mother liquor, sodium chloride, potassium dihydrogen phosphate, and peptone. See Table 2 for specific examples (coded as base 1 to base 4).

[0057] Table 2 basal medium composition mass percentage%

[0058]

[0059] The mother liquor in Table 2 (codenamed mother 1 to mother 4) is a mixed solution of beef extract and pig st...

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Abstract

The invention discloses a quick detection reagent for the drug sensitivity of mycoplasma pneumoniae and a preparation method. The quick detection reagent comprises two parts of a pneumonia type mycoplasma culture medium and a drug sensitive plate. The pneumonia type mycoplasma culture medium comprises fresh bovine serum, fresh yeast juice, glucose, phenol red and bacteriostatic agent. By controlling the pH value and the concentration of the glucose and the phenol red in a culture solution, the reaction speed of the reagent is enhanced. The drug sensitive plate is a 24-hole culture plate and is marked with numbers of 1-12, each number is marked with a hole A and a hole B, and the total number of the holes is 24. The numbers of 1-11 are respectively coated with 11 medicines, and the hole A and the hole B of each number are respectively coated with the high-concentration liquor and low-concentration liquor of the same medicine. The invention has stable performance, visual judgment standard and quick reaction and can report a result within 24 hours. The invention can quickly detect mycoplasma pneumoniae, can detect that the infected mycoplasma pneumoniae is sensitive to which medicine, and has a direct guide function to the pharmacy treatment. The invention provides a guarantee for the quick diagnosis and the effective treatment of the infection disease of mycoplasma pneumoniae.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent and a preparation method, in particular to a drug sensitivity detection reagent and a preparation method for detecting respiratory tract and lung diseases caused by mycoplasma. Background technique [0002] Mycoplasma pneumoniae is a common pathogen of respiratory tract and pulmonary infectious diseases, and the detection rate accounts for 20% to 40% of the population with respiratory tract infection. Mycoplasma pneumoniae infection can cause upper respiratory tract infection and mycoplasma pneumonia, and its medication method is different from that of viral and bacterial infections. The sensitivity of Mycoplasma pneumoniae infected by different individuals to drugs is also different. Ensure early diagnosis and scientific medication of mycoplasma pneumoniae, so that patients can receive timely and effective treatment. At present, there are many experimental diagnostic techniques for mycoplasma at...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18C12Q1/04C12R1/35
Inventor 黄威权黄新凤姜洪波
Owner 黑龙江省汇力医疗器械有限公司
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