Method for quickly culturing anaerobic hydrogen-producing granular sludge

A technology of granular sludge and sludge, applied in the field of biological hydrogen production, can solve the problems of death of anaerobic bacteria, low hydrogen production rate, sludge floating, etc., and achieve the effect of solving the long start-up time and improving the hydrogen production rate.

Inactive Publication Date: 2010-07-07
SICHUAN UNIV
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AI Technical Summary

Problems solved by technology

At present, the method of cultivating hydrogen-producing granular sludge is to directly use anaerobic sludge as seed sludge, but due to the presence of a large number of methanogenic bacteria in anaerobic sludge, most of the hydrogen produced in the process of hydrolysis and acidification is used by them, so many times it will The

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Sludge pretreatment: Take the remaining activated sludge from the urban sewage plant and add 1000mg / L of waste sucrose for cultivation, stir regularly for 5-10 minutes every hour, change 50% of the water after precipitation every day, continuously cultivate for about a week, and add Cationic polyacrylamide makes the concentration of coagulant in the mixed solution 80mg / L, stirs for 2 minutes to make it evenly mixed, and forms tan flocs with good sedimentation performance;

[0021] 2. Add the sludge from step 1 into the reactor as seed sludge, and the volatile sludge concentration in the reactor is 14.7g / L; the diameter of the anaerobic hydrogen production reactor used is 10cm, and the total volume is 8L, of which the volume of the reaction zone is 3L, reflux zone 2L, both the reaction zone and the reflux zone are counted as the effective reaction zone of the reactor, and the precipitation zone is 3L;

[0022] 3. Preparation of synthetic organic wastewater: add 4g / L w...

Embodiment 2

[0026] 1. Sludge pretreatment: Take the remaining activated sludge from the urban sewage plant and add 1000 mg / L of waste sucrose to cultivate, stir regularly for 5-10 minutes every hour, change 50% of the water after sedimentation every day, and cultivate continuously for about a week. The activated sludge was pretreated by heating and boiling for 40 minutes, adding cationic polyacrylamide to make the coagulant concentration in the mixed solution 60mg / L, stirring for 3 minutes to make it evenly mixed, and forming brown flocs with good sedimentation performance.

[0027] 2. Add the seed sludge pretreated in step 1 to the reactor, and control the concentration of volatile sludge in the reactor to 24.6g / L; the diameter of the anaerobic hydrogen production reactor used is 10cm, and the total volume is 8L. The zone volume is 3L, the reflux zone is 2L, the reaction zone and the reflux zone are both counted as the effective reaction zone of the reactor, and the precipitation zone is ...

Embodiment 3

[0032] 1. Sludge pretreatment: Take the remaining activated sludge from the urban sewage plant and add 1000 mg / L of waste sucrose to cultivate, stir regularly for 5-10 minutes every hour, change 50% of the water after sedimentation every day, and cultivate continuously for about a week. The activated sludge is stored under sealed conditions for one month. After taking it out, add cationic polyacrylamide to make the coagulant concentration in the mixed solution 100mg / L, stir for 1.0 minutes to make it evenly mixed, and form tan flocs with good sedimentation performance. body;

[0033] 2. Add the seed sludge pretreated in step 1 to the reactor, and control the concentration of volatile sludge in the reactor to 18.7g / L; the diameter of the anaerobic hydrogen production reactor used is 10cm, and the total volume is 8L. The zone volume is 3L, the reflux zone is 2L, the reaction zone and the reflux zone are both counted as the effective reaction zone of the reactor, and the precipit...

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Abstract

The invention relates to a method for quickly culturing anaerobic hydrogen-producing granular sludge. The invention is characterized in that excess activated sludge which is cultured and pretreated by 60-100mg/L of cationic polyacrylamide is added to a reactor to ensure that the initial sludge concentration in the reactor is 12-25g/L; by using sugar-containing waste water as the substrate, the biochemical oxygen demand (COD) of intake water is 4000mg/L, the pH value is 7.5-8.5, the total hydraulic retention time in the reactor is gradually reduced from the original 15 hours to 3.5 hours, and the organic volume load is gradually increased from 6-10kgCOD/m<3> to more than 30kgCOD/m<3> in the moderate temperature (34-35 DEG C)condition; and the anaerobic hydrogen-producing granular sludge in which clostridia act as the main part accompanied with small amount of filamentous bacteria and sphaerophorus and the total volume of which accounts for 25-40% of the total volume of the reactor can be formed within 7-15 days. The reactor filled with the granular sludge can continuously produce fermentation gas the hydrogen content of which is 44-56%, the gas yield of the reactor is 10-16m<3>, and the hydrogen-producing efficiency can reach 3.1molH2/mol sucrose.

Description

Technical field: [0001] The invention relates to a method for quickly cultivating anaerobic hydrogen-producing granular sludge, which belongs to the technical field of biological hydrogen production. Background technique: [0002] Anaerobic fermentation biological hydrogen production technology is to obtain renewable clean energy-hydrogen while improving its biodegradability during the anaerobic hydrolysis and acidification process of organic wastewater by controlling shorter hydraulic retention time and lower pH value conditions At home and abroad, a lot of manpower and material resources have been invested in research. However, there are still some problems in this technology, such as low reactor efficiency, low substrate conversion efficiency, and low hydrogen content and yield in the fermented gas produced, so it is difficult to realize industrial production. However, this technology has the advantages of developing new energy, saving energy consumption, and purifying s...

Claims

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Application Information

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IPC IPC(8): C02F3/28
Inventor 刘敏陈滢
Owner SICHUAN UNIV
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